Immortalization and functional characterization of rat arachnoid cell lines

Janson, Christopher G.; Romanova, Liudmila; Hansen, Eric A.; Hubel, Allison; Lam, Cornelius H.

doi:10.1016/j.neuroscience.2010.12.035

Cited by 25 publications

(23 citation statements)

References 34 publications

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“…Bodnar et al (1998) found that transient expression of the hTERT in normal diploid cells could activate the telomerase activity and prolong the cell survival. The hTERT has been successfully introduced to human periodontal ligament cells, osteoblasts, mammary epithelial cells and Spinal arachnoid cell line, (Xiaoxue et al 2004;Zhao et al 2010;Hasegawa et al 2010;Janson et al 2011). Different from the traditional immortalization gene, the process that TERT activates telomerase to induce the immortalization is similar to the physiological process in germline stem cells.…”

Section: Discussionmentioning

confidence: 99%

Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells

Teng

et al. 2014

Braz. arch. biol. technol.

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Section: Discussionmentioning

confidence: 99%

Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells

Teng

et al. 2014

Braz. arch. biol. technol.

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“…5D). The coexpression of these three proteins identifies them as AB cells (Murphy et al, 1991;Janson et al, 2011) and eliminates the possibility that the cells are contaminated with endothelial cells, fibroblasts, astrocytes, or macrophages (Murphy et al, 1991). The cells were also stained intensely positive for prostaglandin D 2 synthase (Fig.…”

Section: Methodsmentioning

confidence: 97%

Drug Transporters on Arachnoid Barrier Cells Contribute to the Blood–Cerebrospinal Fluid Barrier

et al. 2013

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The subarachnoid space, where cerebrospinal fluid (CSF) flows over the brain and spinal cord, is lined on one side by arachnoid barrier (AB) cells that form part of the blood-CSF barrier. However, despite the fact that drugs are administered into the CSF and CSF drug concentrations are used as a surrogate for brain drug concentration following systemic drug administration, the tightjunctioned AB cells have never been examined for whether they express drug transporters that would influence CSF and central nervous system drug disposition. Hence, we characterized drug transporter expression and function in AB cells. Immunohistochemical analysis showed P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) in mouse AB cells but not other meningeal tissue. The Gene Expression Nervous System Atlas (GENSAT) database and the Allen Mouse Brain Atlas confirmed these observations. Microarray analysis of mouse and human arachnoidal tissue revealed expression of many drug transporters and some drug-metabolizing enzymes. Immortalized mouse AB cells express functional P-gp on the apical (dura-facing) membrane and BCRP on both apical and basal (CSF-facing) membranes. Thus, like blood-brain barrier cells and choroid plexus cells, AB cells highly express drug transport proteins and likely contribute to the blood-CSF drug permeation barrier.

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“…It has recently proved possible to culture arachnoid cells in vitro, which express claudin 1 and generate a TEER of ~160 Ω cm 2 with restriction of larger solute permeation (Lam et al , 2012Janson et al 2011 ). Characterization of the expression patterns of drug transporters and enzymes in arachnoid tissue and arachnoid barrier (AB) cells shows expression of both P-gp and breast cancer resistance protein (BCRP); an immortalized cell line of AB cells showed P-gp expression on the apical (dura-facing) membrane, and BCRP on both apical and basal (CSF-facing) membranes (Yasuda et al 2013 ).…”

Section: Arachnoid Epithelial Cellsmentioning

confidence: 99%

In Vitro Models of CNS Barriers

Abbott

Dolman

Yusof

et al. 2013

AAPS Advances in the Pharmaceutical Sciences Series

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This chapter reviews the history and modern applications of isolated preparations of the three main CNS barrier layers and cell culture preparations derived from them. In vitro models give valuable mechanistic information but also provide useful assay systems for drug discovery and delivery programmes. However, it is important to take into account practical issues including species differences and the degree to which the differentiated state of the in vivo barrier is retained. The range of models available is reviewed, with a critical evaluation of their strengths and weaknesses, and guidance in selecting and optimizing a suitable model for particular applications. New understanding of the unstirred water layers and paracellular leak pathway in in vitro preparations gives greater insights into the "intrinsic permeability" of the membrane, and a variety of techniques permit characterization of the transport systems and enzymes contributing to barrier function. Increasingly, aspects of CNS pathology are being modelled in cell culture, aiding the optimization of drug delivery regimes in pathological conditions. Chapter 6In Vitro Models of CNS Barriers IntroductionFrom the earliest demonstration of restricted exchange between the blood and the brain (Ehrlich 1885 ) leading to the modern understanding of the blood-CNS barriers, animal experiments and clinical observations have provided valuable information about the physiology and pathology of the barrier layers. However, obtaining mechanistic information from such studies at the cellular and molecular level is complex and time-consuming, and it is often diffi cult to obtain suffi cient spatial and temporal resolution. The situation was dramatically improved by the introduction of in vitro methods (reviewed in Joó 1992 ). Background and Early HistoryThe fi rst successful isolation of cerebral microvessels (Siakotos and Rouser 1969 ;Joó and Karnushina 1973 ) prepared the way for development of in vitro models of the blood-brain barrier (BBB), which have contributed to current understanding of its physiology, pharmacology and pathophysiology (reviewed in Joó 1992 ). Methods have also been developed for in vitro models of the choroid plexus and of the arachnoid epithelium (blood-CSF barrier, BCSFB). However, this proliferation of in vitro models and techniques causes problems for attempts at comparison between models and transferability of results obtained with different models, and makes it hard for scientists entering the fi eld to select an optimal model for their particular interests. This chapter gives an overview of the current status of the most widely used in vitro CNS barrier models, with an update on an earlier review (Reichel et al. 2003 ), and offers guidance in model selection for specifi c applications, including permeability assay for drugs and "new chemical entities" (NCEs). Isolated brain microvessels were the fi rst model system for studying the BBB in vitro, offering new opportunities to investigate physiological and pathological processes at...

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Immortalization and functional characterization of rat arachnoid cell lines

Cited by 25 publications

References 34 publications

Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells

Telomerase reverse transcriptase mediated immortalization of human bone marrow stromal cells

Drug Transporters on Arachnoid Barrier Cells Contribute to the Blood–Cerebrospinal Fluid Barrier

In Vitro Models of CNS Barriers

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