Immortalized germ cells undergo meiosis in vitro.

Hofmann, Marie‐Claude; Hess, Rex A.; Goldberg, Erwin; Millán, José Luis

doi:10.1073/pnas.91.12.5533

Cited by 187 publications

(147 citation statements)

References 21 publications

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“…A temperature sensitive spermatocyte-like cell line, namely GC-2spd (ts), which allows for p53 nuclear localization at 32°C [19], was overexpressed His-tagged Mta1 and subjected to heat stress stimulation. The apoptotic status was remarkably inhibited in the transfected cells.…”

Section: Discussionmentioning

confidence: 99%

Metastasis tumor antigen 1 is involved in the resistance to heat stress‐induced testicular apoptosis

Bao

et al. 2008

FEBS Letters

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Our previous study documented the expression of Mta1 during spermatogenesis. Here, we present evidence for a possible involvement of Mta1 in the regulation of testicular function, possibly by interacting with p53. A notable decrease of Mta1 expression was revealed at postsurgical day 6, consistent with the previously reported upregualtion of p53 in mouse cryptorchidism. Furthermore, in vitro over-expression of Mta1 could remarkably elevate the resistance capability of spermatogenic tumor cells against heat-induced apoptosis with a marked impairment of p53 expression. These findings indicate that Mta1 may operate as a negative modifier of apoptosis by interacting with p53 during gametogenesis.

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Section: Discussionmentioning

confidence: 99%

Metastasis tumor antigen 1 is involved in the resistance to heat stress‐induced testicular apoptosis

Bao

et al. 2008

FEBS Letters

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“…The hgn/hgn is very unique to the point of that the PGCs arrived into the gonads but degenerated before entering meiosis. Details of the mechanisms regulating the differentiation from PGCs into spermatogonia have not known yet, since the culture system that proceeds this developmental process has not been established completely [9,21,24]. Unknown factors required in this developmental process of the PGCs would be primarily defected or secondarily affected by the dysfunction of testicular somatic cells in the hgn/hgn.…”

Section: Resultsmentioning

confidence: 99%

Temporal and Spatial Distribution of Alkaline Phosphatase Activity in Male Hypogonadic Rat (hgn/hgn) Testis during Postnatal Development.

Suzuki

Inaba

Suzuki

1998

The Journal of Veterinary Medical Science

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ABSTRACT. In male hypogonadic mutant rat (hgn/hgn), gonocytes degenerate and peritubular cells form multiple layers around seminiferous tubules during early postnatal testicular development. Alkaline phosphatase (AP) activity has been used as not only a tracer for the primordial germ cells (PGCs) but also a histochemical marker for the peritubular myoid cells. In the present study, we examined the localization of AP activity during the postnatal testicular development in the hgn/hgn and phenotypically normal (+/+ or +/hgn) rat. In the normal testis, high AP activity was located in the surface of the PGCs on 3 days of age. As the PGCs differentiated into spermatogonia, the AP activity drastically decreased in intensity on 7 days and was completely lost by 12 days. In the hgn/hgn, the PGCs showing high AP activity occupied the inside of dilated seminiferous tubules on 3 and 7 days of age. The luminal AP activity declined gradually by 18 days and disappeared on 21 days, when the germ cells completely degenerated in the hgn/hgn testis. In the normal, high AP activity emerged in a layer of the peritubular cells surrounding the tubules on 7 days and afterwards, indicating that the peritubular cells were differentiating into myoid cells. In the hgn/hgn, the peritubular cells formed multiple layers around the tubules and showed weak AP activity on 3 to 18 days of age. On 21 days of age, high AP activity emerged in a single layer of the peritubular cells directly attached to the basement membrane in the hgn/hgn. These results indicate that the PGCs showing AP activity kept remained at later stage in the hgn/ hgn and that the single layer of mature myoid cells showing high AP activity appeared much later in the hgn/hgn testis than in normal. -KEY WORDS: alkaline phosphatase, hypogonadism, peritubular myoid cell, primordial germ cell, testicular development.J. Vet. Med. Sci. 60(6): 671-679, 1998 cells, the abnormal cell division of the gonocytes, the degeneration of the gonocytes, and the formation of multiple layers of the peritubular cells around the seminiferous tubules [32]. Alkaline phosphatases (APs) are membranelinked enzymes that hydrolyze orthophosphate monoesters at alkaline pH [4]. In normal murine testis, AP activity is predominantly localized in the gonocytes and the myoid cells [2,11,16,22]. In this study, we examine the temporal and spatial distribution of AP activity in the postnatal hgn/ hgn and normal (+/+ or hgn/hgn) testis to investigate the distribution and development of the hgn/hgn gonocytes and peritubular myoid cells. MATERIALS AND METHODS Animals:Male normal and hgn/hgn rats used in this study were obtained from matings between proven +/hgn males and females derived from the 21st generation of hypogonadic rat (HGN) line maintained in our department [29]. The rats were kept in a conventional animal room, and were fed a mixed grain diet and water ad libitum [31].Preparation of cryosectioned testis samples: The rats were sacrificed on 3, 7, 12, 18, 21, and 40 days after birth by overdose ether. Th...

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“…Several experiments have identified physical associations and even cellular junctional attachments between germ cells and Sertoli cells under culture conditions (Smith et al, 1992). Other studies have demonstrated maturation or brief differentiation sequences in vitro (Hofmann et al, 1992(Hofmann et al, , 1994Rassoulzadegan et al, 1993). Often cells remaining in culture retain some morphological characteristics of spermatogonia, such as large nuclear to cytoplasmic ratio, and it seems likely these primitive cells can be maintained in culture for several days or perhaps a few weeks (Kierszenbaum, 1994).…”

Section: Introductionmentioning

confidence: 99%

Culture of mouse spermatogonial stem cells

et al. 1998

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Spermatogenesis occurs within the seminiferous tubules of mammals by a complex process that is highly organized, extremely efficient and very productive. At the foundation of this process is the spermatogonial stem cell that is capable of both self-renewal and production of progeny cells, which undergo differentiation over a period of weeks to months in order to generate mature spermatozoa. It had been thought that germ cells survive only a brief period in culture, generally less than a few weeks. However, an accurate assessment of the presence of spermatogonial stem cells in any cell population has only recently become possible with development of the spermatogonial transplantation technique. Using this technique, we have demonstrated that mouse spermatogonial stem cells can be maintained in culture for approximately 4 months and will generate spermatogenesis following transplantation to the seminiferous tubules of an appropriate recipient. Extensive areas of cultured donor cell-derived spermatogenesis are generated in the host, and production of mature spermatozoa occurs. Cultivation of the testis cells on STO feeders is beneficial to stem cell survival. These results provide the first step in establishing a system that will permit spermatogonial stem cells to be cultivated and their number increased in vitro to allow for genetic modification before transplantation to a recipient testis.

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Immortalized germ cells undergo meiosis in vitro.

Cited by 187 publications

References 21 publications

Metastasis tumor antigen 1 is involved in the resistance to heat stress‐induced testicular apoptosis

Metastasis tumor antigen 1 is involved in the resistance to heat stress‐induced testicular apoptosis

Temporal and Spatial Distribution of Alkaline Phosphatase Activity in Male Hypogonadic Rat (hgn/hgn) Testis during Postnatal Development.

Culture of mouse spermatogonial stem cells

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