Immune analysis of cry1Ab-genetically modified potato by in-silico analysis and animal model

Abstract

The safety of feeding transgenic potato (), resistant to the potato tuber moth, to Wistar rats was examined from an immunological perspective. The genetically modified potato (GMP) was harbouring and genes as target and selectable marker genes, respectively. In-silico analysis reconfirmed that Cry1Ab and NPTII protein sequences have no significant homology to known toxins or known allergens. The Wistar rats were fed a diet containing 20% GMP or its parental control, non-GMP (NGMP), for 90 days. The consumption of GMP food did not affect the growth rate, food intake, food efficiency, and general health status of the rats. There were no significant differences in the serum concentrations of immunoglobulin A (IgA), IgG, IgM, interleukin 6 (IL-6), and IFN- between GMP and NGMP-fed rats. Based on such data, it is concluded that the transgenic potato had no adverse effect on immunity functions of Wistar rats.

Subacute toxicity studies of meals prepared from genetically modified potato overexpressing the StDREB1 or the VvWRKY2 transcription factor in rats

“…The absence of adverse effects in our study are consistent with those described in other studies in which processed GM crops with enhanced agronomic traits have shown the absence of clinical effects or histopathological abnormalities in the organs or tissues of animals 22‐37 . Other safety assessment studies of GMPs showed the absence of negative effects on rats 19,38,39 . In contrast, in some cases, side effects have been detected in GMP‐fed rats.…”

“…Similar results have been described by Zeljenková et al ., 18 who found that consuming 11% and 33% GM maize resulted in insignificant decreases in the amount of food ingested and in average rat weights. In contrast, the study by Rahnama et al 19 . did not show any significant change in the weight of rats in a study evaluating a GMP expressing the cry1Ab gene from Bacillus thuringiensis .…”

“…17 Similar results have been described by Zeljenková et al, 18 who found that consuming 11% and 33% GM maize resulted in insignificant decreases in the amount of food ingested and in average rat weights. In contrast, the study by Rahnama et al 19 did not show any significant change in the weight of rats in a study evaluating a GMP expressing the cry1Ab gene from Bacillus thuringiensis. Likewise, El Sanhoty et al, 20 who fed rats with GMPs expressing the cryV gene from B. thuringiensis found no statistical difference in food intake, daily body weight gain, and feed efficiency between the control group and rats fed with the GMPs.…”

“…[22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37] Other safety assessment studies of GMPs showed the absence of negative effects on rats. 19,38,39 In contrast, in some cases, side effects have been detected in GMP-fed rats. Ewen and Pusztai 40 reported that rats fed with GMPs containing Galanthus nivalis lectin exhibited proliferative and antiproliferative effects in the rats' gut.…”

Creation of Borer Pests Resistance Genetically Engineering Peach (Prunus Persica L.) Plants by Cloning cry1Ab Gene

“…Kumar et al (2010) showed that bioassay method and mortality were effective in identifying lines with intermediate to signi cant resistance against pest attack used. The interest in producing GMO from peaches cloning with Cry1Ab gene is an important scienti c development, where Rahnama et al (2017) pointed out that the consumption of GMP food carrying Cry1Ab had no effect on the growth rate, and general health status of the rats.…”