Immune-modulation effect of Halocynthia aurantium tunic lipid on RAW264.7 cells

Jang, A-yeong; Monmai, Chaiwat; Rod-in, Weerawan; Kim, Jieun; You, SangGuan; Lee, Tae Ho; Park, Woo Jung

doi:10.1007/s10068-021-01017-4

Cited by 7 publications

(4 citation statements)

References 34 publications

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“…Specifically, PGE 2 , a pro-inflammatory lipid mediator produced from arachidonic acid by cyclooxygenase-2 (COX-2), was down-regulated by xylitol treatment as compared to sucrose control. However, the yield of NO, an arginine metabolite produced by inducible nitric oxide synthase (iNOS) [ 19 , 20 ], was not significant in xylitol-treated cells. The inhibition of PGE 2 by xylitol may be relevant to their high radical scavenging ability as previously reported [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Trehalose Inhibits Inflammatory Responses through Mitochondrial Reprogramming in RAW 264.7 Macrophages

Park

Kim

2023

Antioxidants

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Studies reported the beneficial effects of trehalose on metabolic syndromes, hyperlipidemia, and autophagy, but its action mechanisms are still poorly understood. Even though trehalose is digested by disaccharidase and absorbed in the intestine, intact molecules encounter immune cells which form a solid balance between the allowance of nutritive substances and the removal of harmful pathogens. In this regard, the polarization of intestinal macrophages into an anti-inflammatory phenotype through metabolic regulation is emerging as a therapeutic strategy for the prevention of gastrointestinal inflammation. The current study investigated the effects of trehalose on immunological phenotypes, energy metabolism, and LPS-induced macrophage mitochondrial functioning. Results indicate that trehalose reduces prostaglandin E2 and nitric oxide, which are inflammatory mediators of LPS-induced macrophages. In addition, trehalose further significantly suppressed inflammatory cytokines and mediators via energy metabolism reprogramming towards M2-like status in LPS-stimulated macrophages.

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Section: Discussionmentioning

confidence: 99%

Trehalose Inhibits Inflammatory Responses through Mitochondrial Reprogramming in RAW 264.7 Macrophages

Park

Kim

2023

Antioxidants

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“…In other studies, lipids and fatty acids derived from marine sources promoted several immune function effects in macrophages, resulting in immunological enhancement (Han et al, 2018;Lim et al, 2021). Our previous study reported that total lipids isolated from H. aurantium tunic had immune regulatory effects and the neutral lipids, glycolipids, and phospholipids exhibit antiin ammatory effects on RAW264.7 cells (Jang et al, 2021;Jang et al, 2022). They have high concentrations of PUFAs and other fatty acids (Jang et al, 2022).…”

Section: Introductionmentioning

confidence: 93%

Immune enhancement effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic on RAW264.7 macrophages

Jang

Rod-in

Park

2023

Preprint

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The fractionated lipids of Halocynthia aurantium (Pyuridae) have been demonstrated to possess anti-inflammatory properties, but their modulatory properties have not been studied. This study aimed at estimating the immune enhancing effects of fractionated lipids from H. aurantium tunic on macrophage cells. The tunic of H. aurantium was used to isolate total lipids, which were then subsequently separated into neutral lipids, glycolipids, and phospholipids. RAW264.7 cells were stimulated with different concentrations (0.5, 1.0, 2.0, and 4.0%) of each fractionated lipid. The cytotoxicity, the production of NO, the expression of immune-associated genes, and signaling pathways were performed. The neutral lipids and glycolipids significantly stimulated the production of NO and the expression of IL-1β, IL-6, TNF-α and COX-2 in a dose-dependent manner, while phospholipids ineffectively induced NO production and mRNA expression. Furthermore, it was found that both the neutral and glycolipids increased NF-κB p-65, p38, ERK1/2 and JNK phosphorylation, suggesting that these lipids may enhance immunity by activating the NF-κB and MAPK signaling pathways. In addition, H. aurantium lipids-induced TNF-α expression was also decreased by blocking the MAPK or NF-κB signaling pathways. These results suggest that the neutral lipids and glycolipids from H. aurantium tunic have potential as a source of immune-enhancement materials.

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“…Fatty acid compositions of neutral lipids, glycolipids, and phospholipids were determined using gas chromatography (GC)-flame ionization detection (FID) (Perkin Elmer, Waltham, MA, USA) as previously described [31]. Quantification of fatty acid peaks were identified by the comparison of their retention times with heptadecanoic acid (C17:0) as internal standard (Sigma-Aldrich, USA, Cat# H3500).…”

Section: Analysis Of Fatty Acid Profilesmentioning

confidence: 99%

“…Recently, the total lipids of H. aurantium tunic were analyzed the fatty acid compositions, consisting of the most abundant of palmitic acids (21.73±2.16), stearic acids (33.13±3.22), oleic acid (6.78±0.28%), LA (2.72±0.23%), dihomo α-linolenic acid (4.09±0.36), EPA (3.88 ±0.31%), and DHA (3.38±0.34%) [31]. The current results showed the fatty acid profiles of fractionated lipids (neutral lipids, glycolipids, and phospholipids) from the total lipids extracted from H. aurantium tunic (Fig 1).…”

Section: Plos Onementioning

confidence: 99%

Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages

et al. 2022

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Halocynthia aurantium is a marine organism that has been considered a promising source for bio-functional materials. Total lipids were extracted from H. aurantium tunic, and then they were separated into neutral lipids, glycolipids, and phospholipids. In the present study, fatty acid profiles of three lipids and their anti-inflammatory effects in RAW264.7 cells were investigated. Among the lipid classes, phospholipids showed the diversity of fatty acid constituents, compared with the glycolipids and neutral lipids. Three lipids contain different contents of fatty acids depending on the kinds of lipids. The most contents were saturated fatty acids (SFAs, 53–69% of the fatty acids) and monounsaturated fatty acids (MUFAs, 15–17% of fatty acids) and polyunsaturated fatty acids (PUFAs, 14–32% of fatty acids) are followed. H. aurantium lipids not only dose-dependently inhibited nitric oxide production but also reduced the expression of inflammatory cytokine genes such as TNF-α, IL-1β, and IL-6 in LPS-stimulated macrophages. It was also demonstrated that the expression of COX-2 was dose-dependently suppressed. Moreover, H. aurantium lipids decreased phosphorylation of NF-κB p-65, p38, ERK1/2, and JNK, suggesting that three lipids from H. aurantium tunic provide anti-inflammatory effects through NF-κB and MAPK signaling. These results indicate that H. aurantium is a potential source for anti-inflammation.

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Immune-modulation effect of Halocynthia aurantium tunic lipid on RAW264.7 cells

Cited by 7 publications

References 34 publications

Trehalose Inhibits Inflammatory Responses through Mitochondrial Reprogramming in RAW 264.7 Macrophages

Trehalose Inhibits Inflammatory Responses through Mitochondrial Reprogramming in RAW 264.7 Macrophages

Immune enhancement effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic on RAW264.7 macrophages

Anti-inflammatory effects of neutral lipids, glycolipids, phospholipids from Halocynthia aurantium tunic by suppressing the activation of NF-κB and MAPKs in LPS-stimulated RAW264.7 macrophages

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