Immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with bhv1 and pi3 viruses

Townsend, J.; Duffus, W. P. H.; Williams, David L.

doi:10.1016/s0034-5288(18)30931-7

Cited by 16 publications

(4 citation statements)

References 27 publications

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“…The hIFN was received from the National Institutes of Health (catalog number Ga23-902-530) at a concentration of 5 × 10 3 IU/mL of medium. Human IFN has been shown to have biological activity similar to porcine IFN (Cerretti et al, 1986;Townsend et al, 1988), and to serve as an acceptable standard for porcine IFN.…”

Section: Interferonmentioning

confidence: 99%

Quantitative relationship of systemic virus concentration on growth and immune response in pigs.

Greiner

Stahly

Stabel

2000

Journal of Animal Science

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Ninety-six pigs from a herd naive for porcine reproductive and respiratory syndrome (PRRS) virus were weaned (10 +/- 3 d of age), penned individually in isolation rooms, and, at 29 +/- 4 d of age, oronasally inoculated with a 2-mL dose of 10(4.3) JA142 PRRS virus/ mL. Body weight; feed intake; and serum concentrations of PRRS virus, interferon, and alpha1-acylglycoprotein were determined for each pig every 4 d on d -8 to 24 postinoculation to quantify the effect of PRRS exposure on the immune response and growth of pigs. Another objective was to determine whether a quantitative relationship between a measure of systemic (serum) virus concentration and pig growth exists. Serum PRRS virus and interferon peaked at 10(5) virus/mL and 69% protection, respectively, at 4 d postinoculation and then declined steadily. Serum alpha1-acylglycoprotein concentration peaked at 12 d postinoculation. Pig weight gains and feed intake were reduced sharply in the initial 8 d postinoculation and to a lesser degree for 24 d postinoculation. The serum concentration of virus and to a lesser degree serum concentrations of interferon and alpha1-acylglycoprotein were quantitatively related to body weight gain and feed intake. The magnitude of the relationship was dependent on the stage of recovery from PRRS infection. Specifically, each log increase in serum virus concentration was associated with a reduction of 4-d pig gain and feed intake of .047 kg and .189 kg, respectively, in 5.5-kg pigs 4 d postinoculation and .085 kg and .036 kg, respectively, in 12.5-kg pigs at 20 d postinoculation. Based on these data, factors that minimize the systemic presence of a virus in pigs result in improvements in pig growth that are quantitatively related to the degree of systemic virus elimination or minimization.

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Section: Interferonmentioning

confidence: 99%

Quantitative relationship of systemic virus concentration on growth and immune response in pigs.

Greiner

Stahly

Stabel

2000

Journal of Animal Science

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“…Spontaneous secretion of IFN-γ was detected in all unstimulated cultures by both techniques, being higher in cultures from non-infested calves, in which IFN-γ is secreted by cells of the innate immune system [11,12]. Innate IFN-γ production is mainly found in young animals, although older animals may also respond in a similar way [13].…”

Section: Discussionmentioning

confidence: 92%

Evaluation of IFN-γ production in bovine hypodermosis using ELISPOT and ELISA

Cabanelas¹,

López²,

Díaz³

et al. 2016

Vet Med Anim Sci

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Background: Two enzyme immunoassays, ELISPOT and sandwich ELISA, were compared in order to evaluate the production of interferon gamma (IFN-γ) in peripheral blood mononuclear cell (PBMC) cultures from Hypoderma (Diptera: Oestridae) infested cattle. Methods: Cell cultures from Hypoderma-infested (sensitized) and uninfested cattle (non-sensitized) were stimulated with the mitogen phytohemaglutinin A (PHA) and with different H. lineatum antigens, crude larval extract (CLE) and its purified fractions (hypodermin A, B and C). IFN-γ secreting cells (SC) were detected using an ELISPOT test, whereas theIFN-γ levels presented in supernatants from parallel cell cultures were measured by a sandwich ELISA; the same bovine specific IFN-γ antibodies were employed in both tests. Results: The addition of H. lineatum antigens had an immunomodulatory effect on PBMC cells from both infested and uninfested cattle, characterized by suppression in the production of IFN-γ. ELISPOT results showed that hypodermin B was the antigen with major immunosuppressive effect on non-sensitized cultures, while CLE had the strongest impact on previously sensitized cultures. Our results revealed that the ELISPOT showed a high sensitivity allowing the determination of IFN-γ-SC frequencies in non-stimulated cultures; in contrast, the sandwich ELISA was not useful for detecting IFN-γ levels in parallel culture supernatants. Conclusion:The ELISPOT test allows an accurately determination of the frequency of IFN-γ-SC in ex vivo PBMCs without the need for extensive re-stimulation in vitro with antigen or mitogen over long periods of time.

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“…IFN activity of three representative T-cell and B-cell lines and of control bovine rIFN--y was completely abrogated by pH 2 treatment of the supernatant fluid, suggesting that the IFN activity was largely due to IFN--y (data not shown). Previously, pH-labile IFN spontaneously produced by bovine lymphocytes was shown to be completely neutralized by antiserum to bovine IFN--y (38).…”

Section: Resultsmentioning

confidence: 99%

“…Production of IFN by T. parva-infected cell lines was determined by evaluating 24-h supernatants from log-phase cultures at 5 x 105 cells per ml in a biological assay. Twoor fourfold dilutions of supernatants were assayed for their capacity to inhibit the cytopathic effect of Semliki Forest virus (courtesy of R. E. Shope, Yale University) on BT-6 bovine thymus primary cell monolayers, using previously established protocols (25,38). Reference standards of human IFN-a (Ga32-902-530; Research Resources Section, National Institutes of Health, Bethesda, Md.)…”

Section: Methodsmentioning

confidence: 99%

Effects of gamma interferon, tumor necrosis factor alpha, and interleukin-2 on infection and proliferation of Theileria parva-infected bovine lymphoblasts and production of interferon by parasitized cells

DeMartini¹,

Baldwin²

1991

Infect Immun

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Theileria parva is a protozoan parasite that infects bovine B cells and aol and y8 T cells and transforms them into continually proliferating cells. CD4+ T. parva-antigen-specific immune T cells have been shown to produce cytokines in response to stimulation with parasitized cells, and T. parva-infected Iymphocytes produce and consume T-cell growth factors and interleukin-2 (IL-2). To ascertain the role of T-cell cytokines on T. parva infections, we evaluated recombinant gamma interferon (rIFN-y), rIL-2, and tumor necrosis factor alpha (rTNF-a) for their effects on establishment, proliferation, and survival of parasitized cells. The results indicate that neither rIFN-y nor rTNF-at had an enhancing or inhibitory effect on the growth of established T. parva-infected T-cell clones, whereas bovine rIL-2 increased the proliferation of infected B-cell and c40 T-cell clones but not that of y8 T-cell clones. To evaluate the effects of the cytokines on establishment of parasitized cell lines, peripheral blood mononuclear cells were cultured in their presence immediately following infection with T. parva sporozoites. Neither rIFN-y nor rIL-2 altered the proportion of cells initially developing schizonts, but both enhanced establishment of infected cell lines by about twofold. In contrast, rTNF-a resulted in about a 33% decrease in the proportion of schizont-infected cells. Inhibitory effects on establishment of parasitized cell lines by rTNF-a were no longer apparent by 12 days following infection. Tests conducted during this study indicated that T. parva-infected lymphocytes also spontaneously produce IFN that is neutralized by acidic pH treatment. In conclusion, we speculate that none of these T-cell cytokines are likely to have a profound inhibitory effect in vivo on T. parva infections. Instead, IFN-y and IL-2 may facilitate the establishment of infection by T. parva.

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Immune production of interferon by cultured peripheral blood mononuclear cells from calves infected with bhv1 and pi3 viruses

Cited by 16 publications

References 27 publications

Quantitative relationship of systemic virus concentration on growth and immune response in pigs.

Quantitative relationship of systemic virus concentration on growth and immune response in pigs.

Evaluation of IFN-γ production in bovine hypodermosis using ELISPOT and ELISA

Effects of gamma interferon, tumor necrosis factor alpha, and interleukin-2 on infection and proliferation of Theileria parva-infected bovine lymphoblasts and production of interferon by parasitized cells

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