Immune Protective Efficacy of China’s Traditional Inactivated and Attenuated Vaccines against the Prevalent Strains of Pasteurella multocida in Mice

Guan, Liming; Song, Ji-Jian; Xue, Yue; Xia, Ai; Liu, Zhijun; Liu, Si; Li, Mengyun; Zhao, Zhanqin

doi:10.3390/vaccines9101155

Cited by 8 publications

(10 citation statements)

References 36 publications

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“…Therefore, the protective effect of the subunit vaccine under 20LD50 challenge would be higher than that of killed vaccine. Moreover, the rVacJ+rPlpE+rOmpH trivalent vaccine group showed a 100% protection rate; our findings indicated that the trivalent vaccine was better than the monovalent recombinant subunit vaccine (44) and attenuated live vaccine (40,47,48).…”

Section: Discussionmentioning

confidence: 67%

“…But in our study, the rVacJ+rPlpE+rOmpH trivalent vaccine group showed a 100% protection rate. At the same time, previous studies showed that the killed vaccines against P. multocida have a protective effect of 50% to 100% (40,45,46). Our research showed the killed vaccinated group only have 50% protection, Compared to subunit vaccines, the individual immunogenic component as well as antibody level of subunit vaccine is higher than that of killed vaccine.…”

Section: Discussionmentioning

confidence: 91%

“…Regarding "reducing and prohibiting antibodies", vaccines are considered the most effective means of preventing bacterial diseases. Previously, the prevention of P. multocida was mainly vaccinated with live or killed vaccines (40); however, both had some drawbacks and lacked efficacy, prompting the development of new, safe, and effective subunit vaccines (39,41,42). Previous studies have indicated that lipoproteins VacJ, PlpE, and outer membrane protein OmpH recombinant vaccine studies against different serotypes of P. multocida are of interest.…”

Section: Discussionmentioning

confidence: 99%

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Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Xiao²,

Chang³

et al. 2022

Front. Immunol.

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Duck cholera (duck hemorrhagic septicemia) is a highly contagious disease caused by Pasteurella multocida, and is one of the major bacterial diseases currently affecting the duck industry. Type A is the predominant pathogenic serotype. In this study, the genes encoding the lipoproteins VacJ, PlpE, and the outer membrane protein OmpH of P. multocida strain PMWSG-4 were cloned and expressed as proteins in E. coli. The recombinant VacJ (84.4 kDa), PlpE (94.8 kDa), and OmpH (96.7 kDa) proteins were purified, and subunit vaccines were formulated with a single water-in-oil adjuvant, while killed vaccines were prepared using a single oil-coated adjuvant. Antibody responses in ducks vaccinated with recombinant VacJ, PlpE, and OmpH proteins formulated with adjuvants were significantly antigenic (p<0.005). Protectivity of the vaccines was evaluated via the intraperitoneal challenge of ducks with 20 LD50 doses of P. multocida A: 1. The vaccine formulation consisting of rVacJ, rPlpE, rOmpH, and adjuvant provided 33.3%, 83.33%, and 83.33% protection, respectively, the vaccine formulation consisting of three recombinant proteins, rVacJ, rPlpE, rOmpH and adjuvant, was 100% protective, and the killed vaccine was 50% protective. In addition, it was shown through histopathological examination and tissue bacterial load detection that all vaccines could reduce tissue damage and bacterial colonization to varying (p<0.001). These findings indicated that recombinant PlpE or OmpH fusion proteins formulated with oil adjuvants have the potential to be used as vaccine candidates against duck cholera subunits.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Xiao²,

Chang³

et al. 2022

Front. Immunol.

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“…The TSB liquid medium was then shaken at 37 • C at 200 r/min for 16 h, and then diluted 1:100 with TSB medium containing 10% newborn bovine serum and shaken for 16 h at 37 • C. The numbers of E. rhusiopathiae cells were enumerated by plate counts at 12 h. Formaldehyde (0.3% v/v) was added to the bacterial suspension, which was inactivated at 37 • C for 48 h. The fully inactivated bacterial suspension was centrifuged and the supernatant discarded, and resuspended with sterile phosphate-buffered saline (PBS, 0.01 mol/L, pH 7.2), that is, HG-1 inactivated antigen was obtained. The inactivated Oli and Alh vaccines were prepared with previously reported methods (33,39), with a 2:1 ratio of oil adjuvant to HG-1 antigen in oil vaccine preparation, and a 1:4 ratio of Alh to HG-1 antigen in Alh vaccine. The ISA201 vaccine, IMS1313 vaccine, and GEL vaccine were prepared according to their respective adjuvant manufacturer's instructions.…”

Section: Preparation Of the Vaccinementioning

confidence: 99%

Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae

et al. 2022

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In this study, we screened adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae (E. rhusiopathiae). Inactivated cells of E. rhusiopathiae strain HG-1 were prepared as the antigen in five adjuvanted inactivated vaccines, including a mineral-oil-adjuvanted vaccine (Oli vaccine), aluminum-hydroxide-gel-adjuvanted vaccine (Alh vaccine), ISA201-biphasic-oil-emulsion-adjuvanted vaccine (ISA201 vaccine), GEL02-water-soluble-polymer-adjuvanted vaccine (GEL vaccine), and IMS1313-water-soluble-nanoparticle-adjuvanted vaccine (IMS1313 vaccine). The safety test results of subcutaneous inoculation in mice showed that Oli vaccine had the most severe side effects, with a combined score of 35, followed by the ISA201 vaccine (25 points), Alh vaccine (20 points), GEL vaccine (10 points), and IMS1313 vaccine (10 points). A dose of 1.5LD50 of strain HG-1 was used to challenge the mice intraperitoneally, 14 days after their second immunization. The protective efficacy of Oli vaccine and Alh vaccine was 100% (8/8), whereas that of the other three adjuvanted vaccines was 88% (7/8). Challenge with 2.5LD50 of strain HG-1 resulted in a 100% survival rate, demonstrating the 100% protective efficacy of the Oli vaccine, followed by the GEL vaccine (71%, 5/7), IMS1313 vaccine (57%, 4/7), ISA201 vaccine (43%, 3/7), and Alh vaccine (29%, 2/7). Challenge with 4LD50 of strain HG-1 showed 100% (7/7) protective efficacy of the Oli vaccine and 71% (5/7) protective efficacy of the GEL vaccine, whereas the protective efficacy of other three adjuvanted vaccine was 14% (1/7). The Alh and GEL vaccines were selected for comparative tests in piglets, and both caused minor side effects. A second immunization with these two adjuvanted vaccines conferred 60 and 100% protective efficacy, respectively, after the piglets were challenged via an ear vein with 8LD100 of strain HG-1. After challenge with 16LD100 of strain HG-1, the Alh and GEL vaccines showed 40% and 100% protective efficacy, respectively. Our results suggested that GEL is the optimal adjuvant for an inactivated vaccine against E. rhusiopathiae.

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“…The evolution of infectious diseases requires the study of the biological properties of pathogens: morphological, enzymatic, molecular genetics, pathogenic, susceptibility to antibacterial agents, and demands a search for new alternatives for their treatment and prevention. An important area of research is the development of measures and methods for their prevention, including zoonoses -diseases transmitted from animals to humans through direct contact or through food, water, and the environment (Bruchmann et al, 2021;Guan et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Studija znakova patogenosti kod Pasteurella multocida, izolirane iz životinja različitih vrsta

et al. 2022

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A significant number of microorganisms in natural and artificial environments exist in a structured formation – biofilm. This formation attaches to a certain surface, particularly the epithelium. The ability to form a similar structure has been observed in Pasteurella multocida, the causative agent of anthropozoonoses that affect domestic and wild animals, birds, companion animals and humans. The spectrum of pathogenetic action of P. multocida is wide and associated with the development of respiratory and multisystemic pathology, bacteraemia and other manifestations. Timely detection of P. multocida and treatment of the diseases it causes in farm and domestic animals is important to limit economic losses and improve social security. The main objective of this study was to determine the pathogenicity of P. multocida, its ability to form a biofilm, its resistance to antibiotics, and to identify the genes responsible for the formation of dermonecrotic toxin and biofilm formation. The paper presents the results of a study of 11 isolates of P. multocida: six isolates (54.5%) from rabbits, two isolates (18.2%) from dogs, two isolates (18.2%) from cats, and one isolate from pigs (9.2%). In all isolates, the gene ptfA was detected. This gene encodes the formation of type 4 fimbriae and participates in the formation of the biofilm, and the studied cultures in vitro formed a biofilm of different densities. The genome of eight isolates (72.7%) included the toxA gene (provides the formation of dermonecrotic toxin), while 45.4% of isolates had a complete set of the studied signs of pathogenicity, both in phenotypic (biofilm formation, mortality for laboratory animals) and genotypic (presence of toxA, ptfA) traits, and three isolates (27.3%) showed signs of multidrug resistance. The virulence of the toxA-negative isolates of P. multocida was lower than in toxA-positive isolates. The culture with the highest virulence (0.5x 101 CFU) and extreme resistance to antibiotics formed a biofilm of the highest density. The association of the gene in the biofilm-producing mechanism needs further evaluation, and further research is needed to identify the relationships between pathogens in Pasteurella multocida isolates from different species of animals and humans.

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Immune Protective Efficacy of China’s Traditional Inactivated and Attenuated Vaccines against the Prevalent Strains of Pasteurella multocida in Mice

Cited by 8 publications

References 36 publications

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Immunogenicity and protective efficacy of the recombinant Pasteurella multocida lipoproteins VacJ and PlpE, and outer membrane protein H from P. multocida A:1 in ducks

Screening immune adjuvants for an inactivated vaccine against Erysipelothrix rhusiopathiae

Studija znakova patogenosti kod Pasteurella multocida, izolirane iz životinja različitih vrsta

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