Immune Response of Gnotobiotic Piglets against Mycoplasma hyopneumoniae

Muneta, Yoshihiro; Minagawa, Yu; Shimoji, Yoshihiro; Ogawa, Yohsuke; Hikono, Hirokazu; Mori, Yoshihide

doi:10.1292/jvms.70.1065

Cited by 26 publications

(20 citation statements)

References 23 publications

(36 reference statements)

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“…High concentrations of IL-18 were found in rat lung homogenates by ELISA (Jordan et al, 2001) and both pro and mature IL-18 have been detected in human peripheral blood mononuclear cells (Puren et al, 1999). Low IL-18 concentrations in the BALF of un-infected 1-day-old gnotobiotic pigs had increased significantly by 2 and 4 weeks following infection with Mycoplasma hyopneumoniae (Muneta et al, 2008). Similar elevations were found 3 days PI in mice inoculated with herpes simplex virus type 1 (Reading et al, 2007) and human macrophages secrete IL-18 after inoculation with H3N2 influenza virus (Pirhonen et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Cytokines and acute phase proteins associated with acute swine influenza infection in pigs

Barbé

Atanasova

Reeth

2011

The Veterinary Journal

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This study set out to investigate the cytokines and acute phase proteins (APPs) associated with the acute stages of experimentally-induced swine influenza virus (SIV) infection in 3-week-old, colostrum-deprived, caesarean-derived piglets. The piglets were inoculated intratracheally with 10(7.5) 50% egg infective dose [EID(50)] Swine/Belgium/1/98 (H1N1) SIV and were euthanased at time-points between 0 and 120h post-inoculation (PI). Broncho-alveolar lavage fluid (BALF), lung homogenates and sera were examined for inflammatory mediators by bioassay or ELISA. Interferon (IFN)-α, interleukin (IL)-6, IL-1 and tumour necrosis factor (TNF)-α peaked in BALF 24-30h PI, when virus titres and the severity of clinical signs were maximal. Whereas IFN-γ and IL-12, but not IL-18, increased in tandem in BALF, serum cytokine concentrations were either undetectable or were up to 100-fold lower. The APP C-reactive protein (CRP) and haptoglobin peaked 24h later than the cytokines and reached higher levels in serum than in BALF. In contrast, lipopolysaccharide (LPS)-binding protein (LBP) only increased in BALF. Lung virus titres tightly correlated with BALF IFN-α, IL-6, IL-1, TNF-α, IFN-γ and IL-12, as well as with serum IL-6, IFN-α and IFN-γ. Signs of disease correlated with the same cytokines in BALF and serum, as well as with BALF LBP and serum CRP. The findings suggest that IFN-γ and IL-12 play a role in the pathogenesis of SIV and that APPs are induced by cytokines. This influenza infection model may have value in assessing the therapeutic potential of cytokine antagonists.

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Section: Discussionmentioning

confidence: 99%

Cytokines and acute phase proteins associated with acute swine influenza infection in pigs

Barbé

Atanasova

Reeth

2011

The Veterinary Journal

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“…Contrarily, previous studies have reported a higher IgG1 than IgG2 antibody response in pigs challenged with M . hyopneumoniae at local [36, 37] and systemic [36] levels. Although reasons for such inconsistencies are unknown, while in such previous studies IgG1 and IgG2 subclasses against P46 [37] or P97 [36] proteins were detected, in the present case, detected antibodies were directed to a recombinant M .…”

Section: Discussionmentioning

confidence: 99%

Potential use of local and systemic humoral immune response parameters to forecast Mycoplasma hyopneumoniae associated lung lesions

et al. 2017

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Immunopathological events are key for the development of enzootic pneumonia (EP), which is macroscopically observed as cranioventral pulmonary consolidation (CVPC). This study aimed to investigate the putative association between the humoral immune response against Mycoplasma hyopneumoniae (M. hyopneumoniae) and prevalence and extension of CVPC in 1) experimentally infected pigs, 2) slaughtered pigs and 3) sequentially necropsied pigs in a longitudinal study. CVPC was scored by means of the European Pharmacopoeia recommended methodology. Specific IgG, IgG1 and IgG2 antibodies were assessed in serum. In addition, mucosal IgG and IgA antibodies were analyzed in broncho-alveolar lavage fluid (BALF) from experimentally challenged pigs. The systemic humoral immune response in experimentally infected pigs was delayed in onset whereas humoral respiratory mucosal immune response appeared more rapidly but declined earlier. Although low, BALF IgG antibodies showed the highest correlation with CVPC scores (r = 0.49, p<0.05). In slaughter-aged pigs, both percentage of lungs with CVPC and mean lung lesion score were significantly higher in M. hyopneumoniae seropositive farms compared to the seronegative ones (p<0.001). Similarly, seropositive sequentially necropsied pigs showed more severe CVPC than seronegative ones. Overall, mean serological values might help to forecast prevalence and severity of EP-like lung lesions using a population based approach. Remarkably, the specific systemic humoral immune response was found to be predominated by the IgG2 subclass, suggesting a dominant Th1-mediated immune response to M. hyopneumoniae.

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“…; Muneta et al . ). However, when co‐infected with the porcine reproductive and respiratory syndrome virus (PRRSV), the severity and duration of the pneumonia become worse (Thacker et al .…”

Section: Introductionmentioning

confidence: 97%

Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

Shimazu

Borjigin

Katayama

et al. 2013

Animal Science Journal

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We recently developed a Landrace line that is resistant to mycoplasmal pneumonia of swine (MPS) infection by genetic selection for five generations, and we reported that the immunophenotype of this line is different from that of the non-selected line in terms of changes in peripheral blood leukocyte population after MPS vaccination. This study followed up previous findings demonstrating changes in soluble factors in blood, namely, hormones, Mycoplasma hyopneumoniae-specific immunoglobulin G (IgG), and cytokines. These two lines were injected with MPS vaccine on days -7 and 0 after blood sampling on those days, and blood samples were collected on days -14, -7, 0, 2, 7 and 14. We found changes in the levels of many hormones and cytokines in both lines. However, we found that only growth hormone (GH) and interferon (IFN)-γ levels were statistically different between these two lines. GH concentration was reduced (day 0) and IFN-γ concentration was increased (day 14) in the MPS-selected line compared with the non-selected line, despite unchanged IFN-γ messenger RNA expression in blood cells. Although detailed mechanisms underlying these phenotypes remain unsolved, these traits would be useful to improve MPS resistance in pig production and provide an insight into MPS infection.

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Immune Response of Gnotobiotic Piglets against Mycoplasma hyopneumoniae

Cited by 26 publications

References 23 publications

Cytokines and acute phase proteins associated with acute swine influenza infection in pigs

Cytokines and acute phase proteins associated with acute swine influenza infection in pigs

Potential use of local and systemic humoral immune response parameters to forecast Mycoplasma hyopneumoniae associated lung lesions

Genetic selection for resistance to mycoplasmal pneumonia of swine (MPS) in the Landrace line influences the expression of soluble factors in blood after MPS vaccine sensitization

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