Immune response of neonates elicited by somatic transgene vaccination with naked DNA

Bona, Constantin A.

doi:10.2741/a181

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1999

2007

Publication Types

Select...

Article3

Book2

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 30 publications

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Combining DNA and protein vaccines for early life immunization against respiratory syncytial virus in mice

Martínez

Li²,

Kovařík

et al. 1999

Eur. J. Immunol.

View full text Add to dashboard Cite

Early life responses to respiratory syncytial virus (RSV)‐F DNA and RSV‐F protein immunization were studied in murine models of neonatal immunization. RSV‐F DNA induced similar antibody (Ab) responses, antigen‐specific IFN‐γ production and cytotoxic T lymphocyte (CTL) responses in 1‐week‐old and adult BALB / c mice. In contrast, RSV‐F protein induced much higher IL‐5 responses in early life. Both vaccines elicited Ab and CTL responses in spite of maternal Ab, but with distinctive kinetics. Sequential RSV‐F DNA priming / protein boosting primed 1‐week‐old mice for RSV‐F‐specific CTL responses, reduced IL‐5 production and enhanced Ab responses. In contrast, IL‐5 exceeded IFN‐γ responses when young mice were primed with protein and boosted with DNA. Last, when protein and DNA immunization were combined, a single vaccine dose induced early Ab responses, preferential IL‐5 responses but strong CTL responses. Sequential or combined DNA / protein immunization thus represent interesting strategies for early life immunization.

show abstract