Immune Response of Rainbow Trout (Oncorhynchus mykiss) to Selected Antigens of Yersinia ruckeri

Abstract

In this study, effects of Yersinia ruckeri antigens on the immune mechanisms of rainbow trout (Oncorhynchus mykiss) were examined. The weight of the 120 fish used in this study was 20-30 g. After injecting 1 mg of formalin-inactivated whole cells (FKC) and O-antigen (Ag-O) intraperitoneally, blood was taken from the caudal vein of anaesthetized fish and metabolic activity of leukocytes (Nitroblue tetrazolium (NBT) activities), phagocytic activity (PA), phagocytic index (PI), serum protein and serum total immunoglobulin (TIg) levels were determined on day 30 after the first immunization. The same procedure was conducted in the control group. In all the experimental groups, considerable increases in the immune indicators were found and significant differences detected between the control and experimental groups (p < 0.05). Metabolic activity of leukocytes decreased significantly (p < 0.05) during the following treatment with antigens compared to the control fish group.

Kinetics of reactive oxygen species produced by rainbow trout (Oncorhynchus mykiss) leukocytes and the effect of the antioxidant astaxanthin

“…It is shorter than that reported in the literature to evaluate the "respiratory burst" which is usually 45 min (Valenzuela et al 2005) or 60 min (Secombes 1990;Logambal et al 2000;Puangkaew et al 2004). This 20 min incubation time is even shorter than that of 30 min used by other authors (Anderson and Siwicki 1993;Kumari and Sahoo 2006;Ispir et al 2008). Therefore, the contribution of this work is to report the kinetics of this reaction, providing a solid base to define the incubation time for future ROS measurements in rainbow trout.…”

“…Formazan produced by NBT reduction mediated by ROS has traditionally been measured after 30 (Anderson and Siwicki 1993;Kumari and Sahoo 2006;Ispir et al 2008), 45 (Valenzuela et al 2005) or 60 min incubation (Secombes 1990;Logambal et al 2000;Puangkaew et al 2004), but we have not been able to find justification for the incubation time used. Therefore, study of formazan formation kinetics was necessary to determine the optimal incubation time.…”

“…A decrease in ROS production can negatively affect the antibacterial activity of the host (Secombes 1996), demonstrating their importance to eliminate pathogenic microorganisms. Several substances can increase or decrease ROS production (Sakai 1999;Puangkaew et al 2004;Kumari and Sahoo 2006;Ispir et al 2008), thus positively or negatively affecting the ability of the animal to protect itself against an infection. As mentioned above, a decrease in ROS may turn an organism more susceptible to infection.…”

Effect of Apricot Kernel Oil on Serum Response of Rainbow Trout (Oncorhynchus mykiss)

“…Lysozyme activity was calculated following the method described by Zhang et al (2008) with a slight change. The total protein level was measured through the Biuret method (Siwicki et al, 1994;Ispir et al, 2009). According to the method previously published by Siwicki et al (1994) The total immunoglobulin level was determined by following.…”

Casualty Agent (Yersiniosis) Detection in Reared Rainbow Trout (Oncorhynchus mykiss) in Kohgiluyeh-Va-Boyerahmad Province in Iran

“…The disease has been called in other word such as: Yersiniosis, Yersinic septicemia, Hagerman-redmouth-disease, etc. ERM produces high mortalities and severe economic losses on fish farms [5].…”