Immune response to Haemonchus contortus and Haemonchus placei in sheep and its role on parasite specificity

Santos, Michelle Cardoso dos; Xavier, Jorge K.; Amarante, Mônica Regina Vendrame; Bassetto, César Cristiano; Amarante, Alessandro Francisco Talamini do

doi:10.1016/j.vetpar.2014.02.048

Cited by 42 publications

(39 citation statements)

References 25 publications

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“…Details about the parasite isolates, donor animal management, and infection of experimental sheep have been published by Santos et al (2014). Briefly, one group of lambs (n=12) was serially infected 12 times (three times a week; on Mondays, Wednesdays and Fridays for four weeks) with 500 infective larvae (L3) n=6;Group Hc).…”

Section: Collection and Examination Of Nematodesmentioning

confidence: 99%

Differentiation of Haemonchus placei from Haemonchus contortus by PCR and by morphometrics of adult parasites and third stage larvae

Santos

Amarante

Silva

et al. 2014

Rev. Bras. Parasitol. Vet.

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Molecular and morphological methods were evaluated to distinguish between Haemonchus contortus and Haemonchus placei species. A total of 141 H. contortus and 89 H. placei male adult specimens collected from artificially infected lambs were identified individually by PCR analysis, using a species-specific primer pair. These PCR results were used as gold standard for Haemonchus spp. identification. Haemonchus placei presented higher mean spicule and barb lengths than H. contortus (P<0.05). However, some measurements overlapped. For this reason, a discriminate function did not allow the correct identification of 13 H. contortus and one H. placei specimen. The sheath tail length of the third stage larvae (L3), which comprises the distance between the tip of the larval tail and the end of the sheath tail, were measured. Only three of the 485 H. placei larvae (0.619%) had a sheath tail shorter than 85 µm, while only four of the 500 H. contortus larvae (0.8%) presented a sheath tail longer than 85 µm. The results indicated that 6.09% of the male adult specimens would be misclassified based on the discriminate function, while only 0.71% of infective larvae would be misclassified. Therefore, identification of L3 can be used as the first method to indicate the presence of H. placei and/ or H. contortus in a population of domestic ruminants.Keywords: Diagnosis, Trichostrongyloidea, molecular biology, ruminants, epidemiology. ResumoMétodos moleculares e morfológicos foram avaliados para a identificação de Haemonchus contortus e Haemonchus placei. No total, 141 H. contortus e 89 H. placei machos adultos, obtidos de cordeiros artificialmente infectados, foram identificados individualmente por PCR com o emprego de um par de "primers" espécie-específico. Esses resultados da análise por PCR foram considerados como padrão para a identificação das espécies de Haemonchus. Haemonchus placei apresentou valores médios de espículos e ganchos superiores aos de H. contortus (P<0,05). Entretanto, houve sobreposição de alguns valores. Por essa razão, a função discriminante não permitiu a identificação correta de 13 exemplares de H. contortus e de um, de H. placei. Foi medida a cauda da bainha de larvas infectantes (L3), que compreende a distância entre a ponta da cauda da larva e a ponta da cauda da bainha. Apenas três das 485 L3 de H. placei (0,619%) apresentaram a cauda da bainha com medida inferior a 85 µm e somente em quatro das 500 L3 de H. contortus (0,8%) essa medida foi superior a 85 µm. Os resultados demonstraram que 6,09% dos machos adultos seriam identificados erroneamente com base na função discriminante, enquanto a identificação incorreta de L3 seria de apenas 0,71%. Portanto, a identificação de L3 pode ser utilizada como método inicial para indicar a presença de H. placei e/ou H. contortus em uma população de ruminantes domésticos.

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Section: Collection and Examination Of Nematodesmentioning

confidence: 99%

Differentiation of Haemonchus placei from Haemonchus contortus by PCR and by morphometrics of adult parasites and third stage larvae

Santos

Amarante

Silva

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…Haemonchus are blood-sucking parasites that cause a decrease in small ruminant performance and, in extreme cases, death, particularly in young animals and periparturient females, which are more susceptible to infections (Santos et al, 2014). In Australia, the estimated cost to control these parasites in sheep is between US$ 220 (McLeod, 1995) and 500 million (Emery and Wagland, 1991).…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus

Gaínza

Domingues

Pérez

et al. 2015

Industrial Crops and Products

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“…H. contortus demonstrates an impressive capacity to adapt and survive in adverse environmental conditions. It has been demonstrated that the worm population can survive inside the host for more than a year, producing fertile eggs that ensure its preservation in a flock (SANTOS et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Gastrointestinal nematode infections in sheep raised in Botucatu, state of São Paulo, Brazil

Wilmsen

Silva

Bassetto

et al. 2014

Rev. Bras. Parasitol. Vet.

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Gastrointestinal nematode infections were evaluated in sheep raised in Botucatu, state of São Paulo, Brazil between April 2008 and March 2011. Every month, two tracer lambs grazing with a flock of sheep were exposed to natural infection with gastrointestinal nematodes for 28 consecutive days. At the end of this period, the lambs were sacrificed for worm counts. Haemonchus contortus presented 100% of prevalence. The seasons exerted no significant influence on the mean intensity of H. contortus, which ranged from 315 worms in November 2010 to 2,5205 worms in January 2011. The prevalence of Trichostrongylus colubriformis was also 100%, with the lowest mean intensity (15 worms) recorded in February 2011 and the highest (9,760 worms) in October 2009. In the case of T. colubriformis, a significant correlation coefficient was found between worm counts vs. rainfall (r = -0.32; P < 0.05). Three other nematodes species were found in tracer lambs, albeit in small numbers. Their prevalence and mean intensity (in parenthesis) were as follows: Oesophagostomum columbianum 28% (25.2), Cooperia curticei 7% (4.5) and Trichuris spp. 2% (1). In conclusion, the environmental conditions of the area proved to be highly favorable for the year-round transmission of H. contortus and T. colubriformis.Keywords: Haemonchus contortus, Trichostrongylus colubriformis, tracer lamb, nematode burden, seasonal variation. ResumoA ocorrência de infecções por nematódeos gastrintestinais foi avaliada de abril de 2008 até março de 2011em ovinos criados em Botucatu, estado de São Paulo. Todos os meses, dois cordeiros traçadores foram expostos à infecção natural por nematódeos gastrintestinais, durante 28 dias consecutivos, ao pastejar junto com um rebanho de ovelhas. Ao final desse período, os animais foram sacrificados para a identificação e quantificação dos helmintos. Haemonchus contortus apresentou prevalência de 100%. Não houve influência significativa das estações do ano na intensidade média de H. contortus, que variou de 315 vermes em novembro/2010 a 25.205 vermes em janeiro/2011. Trichostrongylus colubriformis também apresentou prevalência de 100% com a menor intensidade média (15 vermes) em fevereiro/2011 e a maior (9.760 vermes) em outubro/2009. No caso de T. colubriformis, houve correlação significativa entre as contagens de vermes x precipitação (r = -0,32; P < 0,05). Outras três espécies de nematódeos foram encontradas nos cordeiros traçadores, no entanto em pequenas quantidades, com as seguintes prevalências e intensidades médias (entre parênteses): Oesophagostomum columbianum 28% (25,2), Cooperia curticei 7% (4,5) e Trichuris spp. 2 % (1). Em conclusão, as condições ambientais da área foram muito favoráveis durante todo o ano para a transmissão de H. contortus e T. colubriformis.

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Immune response to Haemonchus contortus and Haemonchus placei in sheep and its role on parasite specificity

Cited by 42 publications

References 25 publications

Differentiation of Haemonchus placei from Haemonchus contortus by PCR and by morphometrics of adult parasites and third stage larvae

Differentiation of Haemonchus placei from Haemonchus contortus by PCR and by morphometrics of adult parasites and third stage larvae

Anthelmintic activity in vitro of Citrus sinensis and Melaleuca quinquenervia essential oil from Cuba on Haemonchus contortus

Gastrointestinal nematode infections in sheep raised in Botucatu, state of São Paulo, Brazil

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