Immune responses and protection induced by Brucella suis S2 bacterial ghosts in mice

Liu, Jun; Li, Yang; Sun, Yansong; Ji, Xue; Zhu, Li; Guo, Xuejun; Zhou, Wei; Zhou, Bo; Liu, Shuang; Zhang, Ruian; Feng, Luzhao

doi:10.1016/j.vetimm.2015.04.008

Cited by 18 publications

(8 citation statements)

References 36 publications

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“…Besides, another noteworthy phenomenon is that the lysis rate of E. coli (pKF396ML or pKF396ME) after induction was significantly higher than that of S. enteritidis with the corresponding plasmid under the same induction conditions (P < 0Á05), illustrating differences among bacterial species. Similar phenomena have been observed among Gram-negative bacteria other than E. coli (Cai et al 2013;Hu et al 2013;Liu et al 2015;Cao et al 2018), which might be related to the evolution of codon usage by phages that increases the effective use of the host translational machinery (Chithambaram et al 2014a(Chithambaram et al , 2014b, while lysis gene E was derived from coliphage phiX174 that infects E. coli and the expression of lysis gene E via codon optimization of S. enteritidis was capable of lysing S. enteritidis more efficiently (data not shown). Other than these profiles, the mutated system described herein, in theory, can be beneficially performed in batch or fed-batch fermentation systems, as there are many reports of the use of temperature-controlled systems for batch production of BGs (Ra et al 2010;Langemann et al 2016;Meitz et al 2016).…”

Section: Discussionsupporting

confidence: 71%

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Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Gong

Gao

et al. 2020

J Appl Microbiol

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Aims To identify a lambda promoter pL mutant that could extend the thermal stability of the thermo‐inducible λcI857‐pR/pL system and to evaluate the effects of the modified system for the controlled expression of lysis gene E during the production of bacterial ghosts (BGs). Methods and results The promoter pL mutant was identified by random mutagenesis and site‐directed mutagenesis. The results showed that a T → 35C mutation in the pL promoter was responsible for the phenotype alteration. Under the same induction conditions, the lysis rates of the modified lytic system on Escherichia coli and Salmonella enteritidis were significantly lower than that of the control, while the lysis rates of Escherichia coli with the thermo‐inducible lytic system were significantly higher than that of S. enteritidis with the corresponding plasmid (P < 0·05). Conclusions Increasing the heat stability of the thermo‐inducible lytic systems decreased lysis efficiency during the production of BGs. There exist differences in the lysis efficiency of thermo‐inducible lytic systems between different bacterial strains. Significance and Impact of the Study These findings enrich current knowledge about modifications to thermo‐inducible systems and provide a reference for the application of these modified systems for the production of BGs and controlled gene expression in bacteria.

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Section: Discussionsupporting

confidence: 71%

“…2013; Liu et al . 2015; Cao et al . 2018), which might be related to the evolution of codon usage by phages that increases the effective use of the host translational machinery (Chithambaram et al .…”

Section: Discussionmentioning

confidence: 99%

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Gong

Gao

et al. 2020

J Appl Microbiol

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“…In another previous study, sh that were immunized intraperitoneally by genetically produced Edwardsiella tarda showed stronger serum agglutination titer than those immunized by traditional (formalin-killed pathogen) vaccine (Kwon, Yoon, Sung, & Kim, 2006). The immunized cattle by genetically prepared BGs of Brucella suis S2 showed the same titers of IgG, interleukin 4, INF-and T-cells as that showed in the conventional formalin-killed Brucella) immunized cattle (Liu et al, 2015).…”

Section: The Survival Animals After Virulence Challenge Testsupporting

confidence: 53%

Immunological Characterization of the Chemically Prepared Ghosts of Salmonella Typhimurium as a Vaccine Candidate

Rabea

Yassin

Mohammed³

et al. 2021

Preprint

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Background: The purpose of this study is to measure the immunogenic protective response of bacterial ghosts of Salmonella typhimurium in animals. Accordingly, the researchers will be able to do further clinical trials and confirm the efficiency of bacterial ghosts in human vaccination. The targets to be measured are humoral immune response: immunoglobulins elevation (IgG), cellular immune response: granulocytes increment; serum antibacterial activity; success the faeces and liver virulence challenge and High survival rates.Results: The BG vaccine was able to protect 100% of BG subcutaneously vaccinated rats and 75% of BG-adjuvant subcutaneously vaccinated rats. The lowest survival rate was in the BG orally vaccinated group (25%). The maximum level of serum IgG titers as well as serum and faeces bactericidal activity (100% eradication) was exhibited in the subcutaneously vaccinated group with BG-adjuvant followed by the BG subcutaneously vaccinated one. Additionally, the highest granulocytes’ number was observed in the BG-adjuvant subcutaneously immunized group. The bacterial load in liver homogenate eliminated in the subcutaneously vaccinated rats by BG after virulence challenge. Conclusions: The bacterial ghosts of Salmonella enterica serovar Typhimurium that prepared by tween 80 protocol showed an effective vaccine candidate that protected animals, eliminated the virulence in faeces and liver. These findings suggest that chemically induced bacterial ghosts of Salmonella typhimurium can be a promising vaccine.

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“…In another trial, the immunized cattle by genetically prepared BGs of Brucella suis S2 showed the same titers of IgG, interleukin 4, INF-γ, and T-cells as is shown in the conventional (formalin-killed Brucella) immunized cattle [ 30 ].…”

Section: Discussionmentioning

confidence: 83%

Immunological characterization of the chemically prepared ghosts of Salmonella Typhimurium as a vaccine candidate

Rabea

Yassin

Mohammed³

et al. 2022

BMC Vet Res

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Background Bacterial ghosts are the evacuated bacterial cellular membranes from most of the genetic and protein contents which preserved their surface characters. Recently, bacterial ghosts exploited for different biomedical applications, for instance, vaccination. The purpose of this study is to measure the immunogenic protective response of bacterial ghosts of Salmonella Typhimurium in animals and to allow future testing this response in humans. The immunologic response was qualitatively, quantitatively, and functionally measured. We have measured the humoral and cellular immune responses, such as immunoglobulins elevation (IgG), increased granulocytes, serum antibacterial activity, clearance of virulence in feces and liver, and the survival rate. Results The bacterial ghosts’ vaccine was able to protect 100% of subcutaneously vaccinated rats and 75% of adjuvant subcutaneously vaccinated rats. The lowest survival rate was in the orally vaccinated group (25%). The maximum level of serum IgG titers, as well as serum and feces bactericidal activity (100% eradication), was exhibited in the subcutaneously vaccinated group with adjuvant vaccines followed by the subcutaneously vaccinated one. Additionally, the highest granulocytes’ number was observed in the adjuvant vaccine subcutaneously immunized group. The bacterial load in liver homogenate was eliminated in the subcutaneously vaccinated rats after the virulence challenge. Conclusions The bacterial ghosts of Salmonella enterica serovar Typhimurium that prepared by Tween 80 Protocol showed an effective vaccine candidate that protected animals, eliminated the virulence in feces and liver. These findings show that chemically induced bacterial ghosts of Salmonella Typhimurium can be a promising vaccine.

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Immune responses and protection induced by Brucella suis S2 bacterial ghosts in mice

Cited by 18 publications

References 36 publications

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Controlled expression of lysis gene E by a mutant of the promoter pL of the thermo‐inducible λcI857‐pL system

Immunological Characterization of the Chemically Prepared Ghosts of Salmonella Typhimurium as a Vaccine Candidate

Immunological characterization of the chemically prepared ghosts of Salmonella Typhimurium as a vaccine candidate

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