Immune Responses in Chickens Against Lipopolysaccharide of Escherichia coli and Salmonella typhimurium

Sunwoo, Hoon H.; Nakano, T.; Dixon, Walter T.; Sim, J. S.

doi:10.3382/ps.0750342

Cited by 76 publications

(50 citation statements)

References 16 publications

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“…These results indicated that bovine decorin is highly antigenic to chickens. The total IgY concentrations monitored in the egg yolk from the three chickens during the experimental period were found to be relatively constant (9.8 ± 1.5 mg mL -1 , P > 0.05) as reported previously (Sunwoo et al 1996).…”

Section: Resultssupporting

confidence: 56%

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Production of chicken egg yolk antibody (IgY) against bovine proteoglycan

Li¹,

Nakano²,

Chae³

et al. 1998

Can. J. Anim. Sci.

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. 1998. Production of chicken egg yolk antibody (IgY) against bovine proteoglycan. Can. J. Anim. Sci. 78: 287-291. This study was undertaken to examine the immune response of chickens to bovine decorin, which is a proteoglycan bearing a single chain of dermatan sulphate. Three 35-wk-old Single Comb White Leghorn hens were immunized with decorin by intramuscular injection, and eggs produced by each bird were examined by ELISA for antidecorin antibody activity in the yolk. The antibody activity started to increase 7 d after immunization, rapidly increased and remained relatively constant after 35 d. The polyclonal antibody obtained was then examined by ELISA inhibition assay with samples of bovine decorin treated with and without enzymes. Digestion of decorin with papain to cleave its protein core caused a complete loss in antigenicity, while chondroitinase-ABC treatment to cleave its dermatan sulphate chain showed no appreciable effect. This indicates that the protein core but not the dermatan sulphate chain was recognized by the anti-decorin antibodies. Immunohistochemical staining located the antigen in bovine, porcine and chicken tissues. It is concluded that chickens can efficiently produce specific antibodies to the protein core of bovine decorin.Key words: Chicken, proteoglycan, decorin, antibody, immune response Li, X., Nakano, T., Chae, H. S. Sunwoo, H. et Sim, J. S. 1998. Production de l'anticorps du jaune d'oeuf de poule (IgY) en présence de protéoglycane bovine. Can. J. Anim. Sci. 78: 287-291. Nous avons examiné la réponse immunitaire des poules à la décorine, protéoglycane portant une chaîne unique de dermatane sulfate. Après avoir administré une injection intramusculaire de décorine à trois poules Single Comb White Leghorn, âgées de 35 semaines, on a examiné par la méthode ELISA la production d'anticorps antidécorine dans le jaune des oeufs de chacune d'entre elles. L'activité immunologique a commencé à augmenter 7 jours après l'injection, marquant une phase d'accroissement rapide pour se stabiliser plus ou moins au bout de 35 jours. Les anticorps polyclonaux ainsi obtenus étaient ensuite examinés par dosage d'inhibition ELISA en présence d'échantillons de décorine traités ou non par voie enzymatiques. La digestion de la décorine en présence de papaïne pour dégager le noyau protéique s'est concrétisée par une perte totale d'antigénicité tandis que le traitement par la chondroïtinase-ABC pour séparer la chaîne de dermatane sulfate n'a pas provoqué d'effets mesurables. Il semble donc que seul le noyau protéique était reconnu par les anticorps antidécorine. La coloration immunohistologique a permis de localiser l'antigène dans les tissus de bovins, de porcins et de poules. Il ressort de ces essais que la poule peut de façon efficiente produire des anticorps à l'égard du noyau protéique de la décorine bovine.Mots clés: Poulet, protéoglycane, décorine, anticorps, réponse immunitaire Chickens protect their offsprings by transferring maternal immunoglobulin G (IgG) antibodies from serum to egg yolk (Patters...

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Section: Resultssupporting

confidence: 56%

“…Radial immunodiffusion of WSF was performed by the method previously described by Sunwoo et al (1996). Solution A was prepared by mixing 1.2 mL rabbit antichicken IgG with 6.8 mL barbital buffer (50 mM sodium barbital and 10 mM barbital, pH 8.6) and incubating in a 56°C water bath.…”

Section: Radial Immunodiffusionmentioning

confidence: 99%

Production of chicken egg yolk antibody (IgY) against bovine proteoglycan

Li¹,

Nakano²,

Chae³

et al. 1998

Can. J. Anim. Sci.

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“…To confirm that gallinacins participate in the defense against pathogens, it is necessary to examine whether their expression is enhanced in response to microorganisms or their components. Lipopolysaccharide (LPS) is the major cell wall constituent of Gram-negative bacteria such as Escherichia coli and Salmonella (Morrison & Ryan 1987) and can be used to mimic bacterial infections and inflammation (Sunwoo et al 1996, Leshchinsky & Klasing 2003. The goal of this study was to determine the types of gallinacins that play a role in the elimination of pathogens in the follicles of laying hens, specifically, which types of GALs are expressed in ovarian follicles and whether their expression changes with follicular growth in healthy birds.…”

Section: Introductionmentioning

confidence: 99%

Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus)

Subedi¹,

Isobe²,

Nishibori³

et al. 2007

Reproduction

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The aim of this study was to identify the types of gallinacin genes (GALs) expressed in ovarian follicles and to determine the changes in their expression during follicular growth and in response to lipopolysaccharide (LPS). Follicles at different stages of growth were collected from laying hens (nZ5) and LPS-injected hens (nZ3). The expression of GALs in the theca and granulosa layers was examined by semi-quantitative RT-PCR. The expression of GAL-1, -2, -7, -8, -10, and -12 in the theca layer and GAL-1, -8, -10, and -12 in the granulosa layer was identified in white and yellow follicles. The expression of these genes was not changed in the theca and granulosa layers during follicular growth except for a decrease in that of GAL-1 in theca. The expression of GAL-1, -7, and -12 in the theca layer of the third largest follicles was increased in response to LPS at a dose of 1 mg/kg body weight and this increase was induced within 3 h and maintained until 12h postinjection. Granulosa layers did not respond to LPS until 12h injection. These results show that six and four types of GALs are expressed in the theca and granulosa layers of healthy follicles respectively, and their levels do not change with follicular growth except for GAL-1 in theca. Elevated levels of GAL-1, -7, and -12 expression in theca in response to LPS suggest that the theca cells expressing these GALs function to eliminate LPS-containing bacteria.

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“…LPS also mimics the effects of antigenic exposure in the wild because it is derived from pathogens that induce illness in natural populations. More importantly, in birds it elicits an antibody response by the immunized individual and these antibodies are transmitted to egg yolks (Sunwoo et al, 1996). Avian reovirus is a viral infection that affects captive poultry, as well as wild populations of Galliformes (Magee et al, 1993;Jones, 2000).…”

Section: Introductionmentioning

confidence: 99%

Maternal antibodies reduce costs of an immune response during development

Grindstaff

2008

Journal of Experimental Biology

120

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SUMMARYYoung vertebrates are dependent primarily on innate immunity and maternally derived antibodies for immune defense. This reliance on innate immunity and the associated inflammatory response often leads to reduced growth rates after antigenic challenge. However, if offspring have maternal antibodies that recognize an antigen, these antibodies should block stimulation of the inflammatory response and reduce growth suppression. To determine whether maternal and/or offspring antigen exposure affect antibody transmission and offspring growth, female Japanese quail (Coturnix japonica) and their newly hatched chicks were immunized. Mothers were immunized with lipopolysaccharide (LPS), killed avian reovirus vaccine (AR), or were given a control, phosphate-buffered saline, injection. Within each family, one-third of offspring were immunized with LPS, one-third were immunized with AR, and one-third were given the control treatment. Maternal immunization significantly affected the specific types of antibodies that were transmitted. In general, immunization depressed offspring growth. However, offspring immunized with the same antigen as their mother exhibited elevated growth in comparison to siblings immunized with a different antigen. This suggests that the growth suppressive effects of antigen exposure during development can be partially ameliorated by the presence of maternal antibodies, but in the absence of specific maternal antibodies, offspring are dependent on more costly innate immune defenses. Together, the results suggest that the local disease environment of mothers prior to reproduction significantly affects maternal antibody transmission and these maternal antibodies may allow offspring to partially maintain growth during infection in addition to providing passive humoral immune defense.

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Immune Responses in Chickens Against Lipopolysaccharide of Escherichia coli and Salmonella typhimurium

Cited by 76 publications

References 16 publications

Production of chicken egg yolk antibody (IgY) against bovine proteoglycan

Production of chicken egg yolk antibody (IgY) against bovine proteoglycan

Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus)

Maternal antibodies reduce costs of an immune response during development

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