Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

Zhang, Li-Shu; Jin, Ningyi; Song, Yuming; Sun, Yansong; Wang, Hong; Zhan, Dawei; Ma, Hao; Shang, Yupu; Jin, Hongtao; Hong, Baoqing; Li, Chang

doi:10.1007/s11434-006-1571-9

Cited by 5 publications

(1 citation statement)

References 36 publications

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“…The codons and the arrangement of these epitope genes were then optimized. Further study [13] of this designed antigen gene combined with HIV-1 p24 gene in a DNA vaccine showed effective immune responses on rhesus monkeys. In this study, HIV-1 P24 molecule was introduced into the designed antigen as a macromolecule carrier to augment the immunogenecity of this vaccine.…”

Section: Discussionmentioning

confidence: 96%

Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice

et al. 2007

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The epidemic of HIV/AIDS is sweeping across the world. It is of great importance to figure out new ways to curb this disease. Epitope-based vaccine is one of these solutions. In this study, a chimeric gene was obtained by combination of a designed HIV-1 multi-epitope gene (MEG) and HIV-1 p24 gene. A recombinant plasmid pUTA2-MEGp24 was then constructed by inserting MEGp24 gene into the downstream of the promoter (ATI-P7.5x20) of fowlpox virus (FPV) transfer vector pUTA2. The recombinant plasmid and wild-type FPV 282E4 strain were then co-transfected into CEF cells and homologous recombination occurred. A recombinant virus expressing HIV-1 protein MEGp24 was screened by genome PCR and Western blot assay. Large scale preparation and purification of the recombinant fowlpox virus (rFPV) were then carried out. BALB/c mice were immunized intramuscularly with the rFPV for three times on day 0, 14 and 42. Mice were executed and sampled one week after the third inoculation. Anti-HIV-1 antibody in serum and Th1 cytokines in the supernatant of cultured spleen cells were assayed by ELISA. The count of T lymphocyte subsets and the CTL activity of spleen lymphocytes were analyzed by flow cytometry and lactate dehydrogenase (LDH) release assay, respectively. The results showed that HIV-1 specific antibody in serum and increased T lymphocyte subsets (CD4(+) T, CD8(+) T) were detected in the immunization group. CTL target-killing activity and higher secretion of Th1 cytokines (IFN-gamma and IL-2) of spleen lymphocytes stimulated by H-2(d)-restricted CTL peptide were observed in immunized mice. We concluded that the rFPV may induce HIV-1 specific immunity especially cellular immunity in mice.

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Section: Discussionmentioning

confidence: 96%

Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice

et al. 2007

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Low Pressure Gas Percolation Characteristic in Ultra-low Permeability Porous Media

et al. 2010

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Low pressure gas percolation characteristic in ultra-low permeability porous media is investigated in this article through core flow experiments. The results show that the wall-slip layer covers more than 10% of the average porous channel radius on account of minimum pore size when the permeability is below 0.1×10 −3 μm 2 order, and seepage behavior is contrasted to that in mid-high permeability pore media. When the gas pressure is not high enough, the flow regime turns into transitional flow instead of slip flow, and nonlinear relationship between the measured gas permeability and the reciprocal of average pressure exists. The gas measuring permeability experiment would be influenced by the nonlinear relationship. If Klinkenberg-corrected method is applied to speculate the equivalent liquid permeability, the extrapolated value will become less or minus. Simultaneously, actual gas flow velocity at the outlet is beyond the calculated value with Klinkenberg formula. A new gas seepage model based on the general slip boundary condition is derived from the homogenization technique in this article. At last the flow model is examined to be suitable for representing the gas flow behavior in ultra-low permeability media and estimating the absolute permeability from single-point, steady-states measurements.Keywords Slippage effect · Ultra-low permeability porous media · Knudsen number · Gas flow regime · Absolute permeability · Homogenization technique Fund project: National science and technology key special project "Development of large oil and gas fields and coal bed gas" (2008ZX05009).

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Zircon U-Pb age, geochemistry and geological implications of granitoids in Tuerkubantao, Xinjiang

Shi

Wang

et al. 2013

J. Earth Sci.

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Immune responses of a designed HIV-1 DNA vaccine on rhesus monkeys

Cited by 5 publications

References 36 publications

Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice

Construction and characterization of a recombinant fowlpox virus containing HIV-1 multi-epitope-p24 chimeric gene in mice

Low Pressure Gas Percolation Characteristic in Ultra-low Permeability Porous Media

Zircon U-Pb age, geochemistry and geological implications of granitoids in Tuerkubantao, Xinjiang

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