Immune responses of mouse mucosal system to glycated and hydrolysed OVA

Abstract

Advanced glycation endproducts (AGEs) are created during the spontaneous reaction that occurs in the body and during protein processing. AGEs cause chronic hyperglycemia, contribute to vascular and renal dysfunction, and are potential antigens. Our recent work showed the mucosal immune system responses to naive, hydrolysed and glycated ovalbumin (OVA‐H, ‐G) gavages to Balb/C mice. There were no differences between groups in anti‐OVA IgG, IgA serum and fecal titers. Cultured B lymphocytes did not show differences in proliferation during stimulation with OVA; OVA‐G/H. B cell ELISpot showed high expression of specific IgA in inductive tissues from OVA‐G and ‐H group. FACS analysis showed significantly expressed CD4+ in mesenteric LN and PP in OVA‐H. Significant levels of CD4+TGFβ were found in OVA‐G group in PP, MLN, iLP, and in OVA‐H group in SPL, PP and iLP. Sorted CD4+ populations were cultured with OVA, OVA‐H, OVA‐G. It was found significant level of TGF‐β and IL‐4 in culture supernatant. Adding OVA‐G to the culture significantly affected the levels of IL‐17 in culture supernatant. This expression suggests that AGEs could induce T cell population Th17, which is recognized as a mediator in autoimmune pathology. The presence of strong TGF‐β responses, together with significant levels of Il4, may indicate of expression Th3 cells, which provides help for IgA and has suppressive properties for some immune cells. All groups showed positive DTH test responses after 48h. The presented protein modifications modulated mucosal immune response for OVA. Using low dose antigen immunization and modification could be the convenient way to modulate immune responses to protect against food allergies and other diseases. This work was supported by MSHE in Poland, Grant N312 32/0159.

Evaluation of Immunoreactivity of Pea (Pisum sativum) Albumins in BALB/c and C57BL/6 Mice

“…Our experiments show that non-enzymatic glycosylation (glycation) of PA impacts murine immune response against PA. G-PA shows less immunogenicity as it induced a lower specific antibodies titers when compared to PA. These results are similar to those in one of our earlier studies, where we found that glycation has significant effect on the humoral responses elicited against ovalbumin . Probably, the attachment of glucose to albumins or aggregation of glycated proteins has changed availability of the conformational epitopes of PA, thus affecting the production of antibodies; however, this will require further investigations.…”

“…These results are similar to those in one of our earlier studies, where we found that glycation has significant effect on the humoral responses elicited against ovalbumin. 33 Probably, the attachment of glucose to albumins or aggregation of glycated proteins has changed availability of the conformational epitopes of PA, thus affecting the production of antibodies; however, this will require further investigations. Since the humoral response to PA was found to be greater than that to G-PA, we used PA for immunization of mice to check for cellular immune response.…”