Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSF

Li, Zhijun; Gang, Wang; Wang, Yan; Zhang, Chong; Huang, Baicheng; Li, Qiongyi; Li, Liangliang; Xue, Biyun; Ding, Peiyang; Cai, Xuehui; Wang, Chengbao; Zhou, En‐Min

doi:10.1016/j.vetimm.2015.08.003

Cited by 17 publications

(20 citation statements)

References 33 publications

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“…In this study, six recombinant HP-PRRSVs were subjected to western blot by measuring the ratios of the N protein and EGFP protein, respectively. The present results and our earlier data showed that insertion of the EGFP transcriptional units between N gene and 3′-UTR did not affect the incorporation of viral protein into the virions by measuring the ratios of the N protein and EGFP protein [ 20 – 22 ]. Moreover, six recombinant HP-PRRSVs were subjected to Northern blot analysis by measuring the ratios of the EGFP mRNA.…”

Section: Discussionsupporting

confidence: 57%

Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus

Wang

Han

Gao

et al. 2017

Vet Res

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In order to gain insight into the role of the transcription regulatory sequences (TRSs) in the regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus (PRRSV), the enhanced green fluorescent protein (EGFP) gene, under the control of the different structural gene TRSs, was inserted between the N gene and 3′-UTR of the PRRSV genome and EGFP expression was analyzed for each TRS. TRSs of all the studied structural genes of PRRSV positively modulated EGFP expression at different levels. Among the TRSs analyzed, those of GP2, GP5, M, and N genes highly enhanced EGFP expression without altering replication of PRRSV. These data indicated that structural gene TRSs could be an extremely useful tool for foreign gene expression using PRRSV as a vector.Electronic supplementary materialThe online version of this article (doi:10.1186/s13567-017-0445-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 57%

Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus

Wang

Han

Gao

et al. 2017

Vet Res

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“…PRRSV vectors expressing foreign genes from an expression cassette that were inserted between ORF1b and ORF2a have been investigated systematically (27)(28)(29)(30)(31)(32). The recombinant PRRSVs expressing foreign genes between ORF1 and 2a have proven stability and still possess the biological activities of the foreign genes both in vitro and in vivo (27)(28)(29)(30)(31)(32). In the present study, we used the same strategy to test the ability of a cell adapted attenuated PRRSV strain to express GFP from an independent transcription unit.…”

Section: Discussionmentioning

confidence: 99%

“…In this case, a synthetically copied TRS with flanking sequences was placed after the inserted gene to regulate the synthesis of sub-genomic (sg) mRNA of the downstream viral structural protein (25,26). Most recombinant PRRSVs expressing foreign genes including marker genes, immunogenic genes of viruses and cytokine genes between ORF1 and 2 or ORF7 and 3 ′ UTR, show genetic stability and have the biological activity of the foreign genes during serial passages of recombinant viruses both in vitro and in vivo (27)(28)(29)(30)(31)(32).…”

Section: Introductionmentioning

confidence: 99%

Generation of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing Two Marker Genes

Wang

Xie

et al. 2020

Front. Vet. Sci.

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Porcine reproductive and respiratory syndrome virus (PRRSV) has been used as a gene expression vector in the development of vaccines. Most of these recombinant PRRSV vectors express only a single foreign gene through either an internal insertion in the hypervariable region of nsp2 or expression cassette and some of these recombinant vectors are genetically unstable. Here, we combined internal insertion in nsp2 and expression cassette methods to generate a novel recombinant PRRSV stably expressing the red fluorescence protein (RFP) and the green fluorescence protein (GFP) genes. Biological characteristic analysis of the recombinant PRRSV carrying the two marker genes, rGX-RFP-GFP, showed that it displayed similar growth kinetics and yet it yielded less infectious viruses when compared to the parental virus rGXAM. Co-expression of both the RFP and GFP was observed using confocal fluorescence microscopy when the rGX-RFP-GFP viruses infected MARC-145 cells. Furthermore, the PRRSV-based two-marker gene expression vector is genetically stable during 20 serial passages in MARC-145 cells. These data demonstrate that it is possible to express two interested immunogens from a single PRRSV vector.

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“…When the recombinant PRRSV mutants infect the target cells, the immunomodulatory molecules will be expressed which will exert their biological effects on the host immune system. Thus far, PRRSV mutants carrying genes encoding different isotype of IFN, granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukins have been constructed [71][72][73][74]. While the recombinant viruses carrying the immunomodulatory molecules elicit enhanced levels of T-cell responses, they do not confer significantly better protection against challenge infection than the parental strains [71][72][73].…”

Section: Improve Immune Responsementioning

confidence: 99%

“…[ [71][72][73][74] Development of DIVA vaccines by removing dominant B cell epitopes located in the nsp2 and M proteins.…”

Section: Introductionmentioning

confidence: 99%

Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications

Chaudhari

2020

Viruses

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Porcine reproductive and respiratory syndrome virus (PRRSV) is a positive sense, single-stranded RNA virus that is known to infect only pigs. The virus emerged in the late 1980s and became endemic in most swine producing countries, causing substantial economic losses to the swine industry. The first reverse genetics system for PRRSV was reported in 1998. Since then, several infectious cDNA clones for PRRSV have been constructed. The availability of these infectious cDNA clones has facilitated the genetic modifications of the viral genome at precise locations. Common approaches to manipulate the viral genome include site-directed mutagenesis, deletion of viral genes or gene fragments, insertion of foreign genes, and swapping genes between PRRSV strains or between PRRSV and other members of the Arteriviridae family. In this review, we describe the approaches to construct an infectious cDNA for PRRSV and the ten major applications of these infectious clones to study virus biology and virus–host interaction, and to design a new generation of vaccines with improved levels of safety and efficacy.

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Immune responses of pigs immunized with a recombinant porcine reproductive and respiratory syndrome virus expressing porcine GM-CSF

Cited by 17 publications

References 33 publications

Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus

Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus

Generation of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus Stably Expressing Two Marker Genes

Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications

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