Immune responses of prophenoloxidase and cytosolic manganese superoxide dismutase in the freshwater crayfish Cherax quadricarinatus against a virus and bacterium

Liu, Yanting; Chang, Chin-I; Hseu, Jinn–Rong; Liu, Kuan-Fu; Tsai, Jyh-Ming

doi:10.1016/j.molimm.2013.03.023

Cited by 35 publications

(12 citation statements)

References 43 publications

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“…Based on this and other studies, the proPO has been suggested critical for the defence against pathogenic infection in shrimps (Amparyup, Charoensapsri & Tassanakajon 2009;Charoensapsri et al 2011;Fagutao et al 2012). This upregulation of proPO-2 expression is also consistent with previous research in which the proPO gene was found to be upregulated in crustaceans by potential immune stimulants or bacterial challenge (Arockiaraj et al 2013;Hauton, Brockton & Smith 2007;Liu et al 2013;Moseley 2000;.). The upregulation of proPO generally coincided with a high level of immunity and resulted in higher survival (Tobian et al 2004;Hauton et al 2007;Wang et al 2008;Hsieh et al 2013).…”

Section: Discussionsupporting

confidence: 90%

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Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK)

Phuoc

Wille

et al. 2015

Journal of Fish Diseases

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This study was conducted to test the effect of DnaK on priming immune responses in Penaeid shrimp. Juvenile-specific pathogen-free (SPF) P. vannamei shrimp were injected with 0.05 μg recombinant DnaK. One hour post-DnaK priming, a non-lethal dose of Vibrio campbellii (10(5) CFU shrimp(-1)) was injected. Other treatments include only DnaK or V. campbellii injection or control with blank inocula. The haemolymph of three shrimp from each treatment was collected at 1.5, 6, 9 and 12 h post-DnaK priming (hpp). It was verified that injection with DnaK and V. campbellii challenge affected the transcription of 3 immune genes, transglutaminase-1 (TGase-1), prophenoloxidase-2 (proPO-2) and endogenous HSP70 (lvHSP70). In P. monodon, shrimp were first injected with DnaK at a dose of 10 μg shrimp(-1) and one hour later with 10(6) CFU of V. harveyi (BB120) shrimp(-1). Shrimp injected with DnaK showed a significant increase in proPO expression compared to the control (P < 0.05). Yet a double injection (DnaK and Vibrio) seemed to cause an antagonistic response at the level of expression, which was not equalled at the level of PO activity. Those results suggest that DnaK is able to modulate immune responses in P. vannamei and P. monodon.

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Section: Discussionsupporting

confidence: 90%

“…; Hauton, Brockton & Smith ; Liu et al . ; Moseley ;.). The upregulation of proPO generally coincided with a high level of immunity and resulted in higher survival (Tobian et al .…”

Section: Discussionmentioning

confidence: 99%

Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK)

Phuoc

Wille

et al. 2015

Journal of Fish Diseases

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“…Previous studies have also demonstrated that SOD activity increases in hemocytes and muscle cells of L. vannamei treated with an immunopotentiator, and the survival rate increased following bacterial infection, suggesting that SOD regulates the immune response in shrimps (Campa-Cordova et al, 2002). In crustaceans, SOD and phenoloxidase are two enzymes with roles in the immune system, which may be used as indicator enzymes to detect immune function (Amparyup et al, 2013;Liu et al, 2013). Induction of cytMnSOD gene expression by KK-42 is advantageous for adaptation to harsh environments as it strengthens immunity against pathogenic bacteria and viruses and enhances tolerance, which is likely to be one of the molecular mechanisms through which KK-42 increases survival of prawns infected with A. hydrophila.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Imidazole derivative KK-42 is a well-known regulator of insect growth. KK-42 pretreatment has been shown to promote the survival of Macrobrachium nipponense infected with Aeromonas hydrophila, possibly via activation of superoxide dismutase (SOD). In this study, the cytMnSOD gene was cloned from the hepatopancreas of M. nipponense using the rapid amplification of cDNA ends technique. The full-length cDNA of cytMnSOD was 1233 bp long, and the open reading frame was 858 bp long, encoding a 286-aa protein with a 60-aa leader sequence. The calculated molecular mass of the translated cytMnSOD protein was 31.33 kDa, with an estimated isoelectric point of 5.62. cytMnSOD contained two N-glycosylation sites, four conserved amino acids responsible for binding manganese, and a manganese SOD domain (DVWEHAYY). Real-time RT-PCR analysis showed that cytMnSOD was expressed in all tissues examined with the highest expression observed in the hepatopancreas. Levels of the cytMnSOD transcript in the hepatopancreas were highest in stage C of the molting cycle. Real-time PCR analysis revealed that cytMnSOD expression increased significantly 3, 6, and 12 h after KK-42 treatment, with simultaneous increases in SOD activity from 6 to 12 h. Our results demonstrate that cytMnSOD expression and SOD activity may be induced by KK-42, which may represent one of the molecular mechanisms through which KK-42 promotes increased survival of prawns infected with A. hydrophila.

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“…Unlike fish and other vertebrates, crustaceans lacks adaptive immune system and therefore solely rely on innate immune response generated primarily from the immunocompetent cells and mucus of intestinal mucosal membrane 9,70,71 . In the present study, we selected 10 genes that are reported to play a crucial role in innate immune response of crayfish earlier 7,9,[72][73][74] . We found upregulated expression of pro-inflammatory cytokines, and crustacean's hemolymph genes in the probiotic fed marron.…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

Foysal

Fotedar

Siddik

et al. 2020

Sci Rep

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This study aimed to investigate the combined effects of two most potent probiotic bacteria Lactobacillus acidophilus and Lactobacillus plantarum on overall health and immune status of freshwater crayfish, marron under laboratory conditions. A total of 36 marron were distributed into six different tanks and two different feeding groups, control and probiotic-fed group. After acclimation, control group was fed with basal diet while probiotic group was fed 10 9 cfU/mL per kg of bacterial supplemented feed for 60 days. The results showed no significant differences in weight gain, however, probiotic feed significantly enhanced some hemolymph parameters and biochemical composition of tail muscle. Histology data revealed better hepatopancreas health and higher microvilli counts in the marron gut fed probiotic diet. The probiotic bacteria triggered significant shift of microbial communities at different taxa level, mostly those reported as beneficial for crayfish. The probiotic diet also enriched the metabolic functions and genes associated with innate immune response of crayfish. Further correlation analysis revealed significant association of some taxa with increased activity for hemolymph and immune genes. therefore, dietary Lactobacillus supplementation can modulate the overall health and immunity as well as gut microbial composition and interaction network between gut microbiota and immune system in crayfish. Aquaculture has become an important food sector for meeting the overall protein demand for growing population. The global consumption of crustaceans has increased greatly and especially those with live transport abilities are increasingly becoming popular 1-3. Marron (Cherax cainni) is one of the largest freshwater crayfish farmed in Western Australia (WA) that has high nutritive value and widespread consumer preferences 4,5. In addition, long distance live transportation ability of marron further increases its international demand, and thus has become an ideal crayfish species for commercial farming 6,7. However, the production of marron in WA has remained stagnant for a long time 7. Selection of proper diets and maintaining the optimum water quality are the two most crucial factors required for marron farming 5,7. Although no disease outbreaks have yet been reported, the interest and intend of intensification in marron farming can expose marron to possible crayfish pathogens including Vibrio, Aeromonas and Rhodobacter 5,6. In the past, several laboratory based trials have been conducted 3,6-9 , however, finding a suitable diet and identifying the beneficial bacteria that potentially can influence the growth and immune performance of marron has still remained unknown. Towards sustainable development of aquaculture, the use of feed additives including probiotics, prebiotics, synbiotics, parabiotics and phytogenics in crustacean's diet to boost biological indices has gained extensive attention from the researchers and farmers. Probiotics are microorganisms associated with health and immune benefits for the host ...

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Immune responses of prophenoloxidase and cytosolic manganese superoxide dismutase in the freshwater crayfish Cherax quadricarinatus against a virus and bacterium

Cited by 35 publications

References 43 publications

Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK)

Priming the immune system of Penaeid shrimp by bacterial HSP70 (DnaK)

Molecular characterization and upregulation of cytosolic manganese superoxide dismutase by imidazole derivative KK-42 in Macrobrachium nipponense

Lactobacillus acidophilus and L. plantarum improve health status, modulate gut microbiota and innate immune response of marron (Cherax cainii)

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