Immune Responses to Indwelling Medical Devices

Nymer, M.; Cope, Emily K.; Brady, Rebecca A.; Shirtliff, Mark E.; Leid, Jeff G.

doi:10.1007/7142_2008_4

Cited by 3 publications

(3 citation statements)

References 104 publications

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“…Implanted medical devices have been consistently shown to improve the quality of life of patients suffering from critical conditions such as the destruction of joints [1]. Increasing life span and evolving medical sciences have accelerated the use of such devices.…”

Section: Introductionmentioning

confidence: 99%

“…Elderly, obese, malnourished, diabetic and rheumatic patients and near-surface body inserts with poor soft tissue coverage are most vulnerable to such infections. Medical device-associated infections are common complications of implantation surgery caused by material-colonizing microbial communities, also known as biofilms [1].…”

Section: Introductionmentioning

confidence: 99%

“…After implantation, medical devices are promptly coated by host proteins, involving fibrinogen, fibronectin and fibrin, which can facilitate adhesion of bacteria like Staphylococcus aureus that express receptors for binding these plasma proteins [16,17]. The implant-induced changes in the host, such as reduced blood flow and impaired activity of lymphocytic and phagocytic cells, are other factors that can also promote the biofilm formation on medical devices [1]. Besides, some substrate materials such as polymethyl methacrylate, acrylic glass (PMMA) can intrinsically contribute to biofilm formation by hampering the complement and reducing leukocytic activity.…”

Section: Introductionmentioning

confidence: 99%

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Structural and Functional Dynamics of Staphylococcus aureus Biofilms and Biofilm Matrix Proteins on Different Clinical Materials

et al. 2019

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Medical device-associated staphylococcal infections are a common and challenging problem. However, detailed knowledge of staphylococcal biofilm dynamics on clinically relevant surfaces is still limited. In the present study, biofilm formation of the Staphylococcus aureus ATCC 25923 strain was studied on clinically relevant materials—borosilicate glass, plexiglass, hydroxyapatite, titanium and polystyrene—at 18, 42 and 66 h. Materials with the highest surface roughness and porosity (hydroxyapatite and plexiglass) did not promote biofilm formation as efficiently as some other selected materials. Matrix-associated poly-N-acetyl-β-(1-6)-glucosamine (PNAG) was considered important in young (18 h) biofilms, whereas proteins appeared to play a more important role at later stages of biofilm development. A total of 460 proteins were identified from biofilm matrices formed on the indicated materials and time points—from which, 66 proteins were proposed to form the core surfaceome. At 18 h, the appearance of several r-proteins and glycolytic adhesive moonlighters, possibly via an autolysin (AtlA)-mediated release, was demonstrated in all materials, whereas classical surface adhesins, resistance- and virulence-associated proteins displayed greater variation in their abundances depending on the used material. Hydroxyapatite-associated biofilms were more susceptible to antibiotics than biofilms formed on titanium, but no clear correlation between the tolerance and biofilm age was observed. Thus, other factors, possibly the adhesive moonlighters, could have contributed to the observed chemotolerant phenotype. In addition, a protein-dependent matrix network was observed to be already well-established at the 18 h time point. To the best of our knowledge, this is among the first studies shedding light into matrix-associated surfaceomes of S. aureus biofilms grown on different clinically relevant materials and at different time points.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%