Larval Settlement and Surfaces: Implications in Development of Antifouling Strategies

Murthy, P. Sriyutha; Venugopalan, V.P.; Nair, K.V.K.; Subramoniam, T.

doi:10.1007/978-3-540-69796-1_13

Cited by 12 publications

(2 citation statements)

References 134 publications

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“…However, they have also reported that exposure to norepinephrine or epinephrine led the oyster larvae to metamorphose directly without attaching to any surface, thereby bypassing the dopaminergic pathway. This theory was later supported by various pharmacological studies [8,11,37,38]. However, there is a lack of molecular evidence to connect these findings to actual pathways.…”

Section: Introductionmentioning

confidence: 90%

Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas

et al. 2022

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The molecular mechanisms underlying the conspecific cue-mediated larval settlement in Crassostrea gigas is not yet fully understood. In this study, we described and compared the transcriptomes of competent pediveligers (Pedi) and conspecific cue-induced postlarvae (PL). A total of 2383 candidate transcripts were identified: 740 upregulated and 1643 downregulated transcripts, after settlement. Gene Ontology analysis revealed active chitin binding, calcium ion binding, and extracellular region processes in both stages. Results showed that the differential expression trend of six candidate transcripts were consistent between the quantitative real-time PCR and transcriptome data. The differential transcript expression related to shell formation showed closely linked dynamics with a gene regulatory network that may involve the interplay of various hormone receptors, neurotransmitters, and neuropeptide receptors working together in a concerted way in the Pedi and PL stages. Our results highlight the transcriptome dynamics underlying the settlement of oysters on conspecific adult shells and demonstrate the potential use of this cue as an attractant for wild and hatchery-grown oyster larval attachment on artificial substrates. It also suggests the possible involvement of an ecdysone signal pathway that may be linked to a neuroendocrine-biomineralization crosstalk in C. gigas settlement.

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Section: Introductionmentioning

confidence: 90%

Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas

et al. 2022

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“…Chemoreception involves the binding of chemicals to receptors in the neural tissues of larvae, which activate neuronal networks (Hay 2009). Factors which regulate larval settlement behavior have been investigated extensively for many marine taxa (reviewed by Hadfield and Paul 2001;Murthy et al 2009), but the complex chemoreception process has yet to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms

2011

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Please cite this article as: Alfaro, Andrea C., Young, Tim, Ganesan, Annapoorna Maitrayee, Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms, Aquaculture (2011), doi: 10.1016/j.aquaculture.2011.08.038 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Pseudoalteromonas sp. AMGP1 were tested for their abilities to induce larval settlement. Toxicity effects of each treatment also were simultaneously identified by recording larval mortalities. Results indicate that all chemicals used induced larvae to settle, with acetylcholine being the most effective (~24% at 10 -6 M compared to <2% in control assays). Toxicities of treatment compounds were low at optimal settlement inducing concentrations, except for L-Tryptophan (~32%) and GABA (~59%). Our data suggest that catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. While serotonin precursors did induce low levels of larval settlement at some concentrations, high toxicity responses to 5-HTP at 10 -5 M, combined with complete settlement inhibition indicate A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTthat the mechanism of action may be more complex than can be elucidated in this study. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases across all three strains, and settlement inhibition was observed when larvae were exposed to biofilm exudates of all bacterial strains. Comparisons of A. maoriana responses to other endemic and worldwide distributed mussel species are provided as a means to highlight potential evolutionary differences in chemoreception mechanisms. Highlights 1.Mussel larval settlement responses were tested with neuroactive compounds amino acids, and bacterial biofilms.2. All chemicals induced larvae to settle, with acetylcholine being the most effective.3. Catecholamines (and their precursors) play an important role in the biochemical mechanisms of settlement for A. maoriana. Settlement responses to serotonin precursors require further investigationsince there were high toxicity responses.5. Larval settlement responses to bacterial treatments were low for planktonic and biofilm phases, and exposure to biofilm exudates resulted in settlement inhibition.

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Active Enzyme Nanocoatings Affect Settlement of Balanus amphitrite Barnacle Cyprids

Tasso

Conlan

Clare

et al. 2011

Adv Funct Materials

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Balanus amphitrite cyprids produce complex adhesive substances that enable their attachment to surfaces and impart a strong detachment resistance from most immersed substrata. The colonization of man‐made structures by barnacle cyprids and other marine organisms is a troublesome and costly phenomenon, for which controlling strategies are actively sought. In this work, we expand previous investigations about the susceptibility of cyprid adhesives to unpurified proteases in solution by evaluating the interplay between these secreted biomolecules and a surface‐confined purified protease. The strategy involved the covalent immobilization of the enzyme Subtilisin A to maleic anhydride copolymer thin films through the spontaneous reaction of anhydride moieties with lysine side chains. This enabled the production of bioactive layers of tunable enzyme surface concentration and activity, which were utilized to systematically evaluate the effect of the immobilized enzyme on cyprid settlement and exploratory behavior. Surfaces of increasing enzyme activity displayed a gradual decrease in cyprid settlement levels (approaching inhibition) as well as an increase in post‐settlement adhesion failure (evidenced by significant numbers of detached metamorphosed individuals). High activities of the bound enzyme also affected pre‐settlement behavior of cyprids, reducing the velocity and total distance moved while increasing the amount and speed of meander compared to the controls. The here‐reported low enzyme surface concentrations found to be remarkably effective at reducing cyprid settlement hold promise for the use of immobilized enzymes in the control of marine biofouling.

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Larval Settlement and Surfaces: Implications in Development of Antifouling Strategies

Cited by 12 publications

References 134 publications

Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas

Transcriptome Dynamics of an Oyster Larval Response to a Conspecific Cue-Mediated Settlement Induction in the Pacific Oyster Crassostrea gigas

Regulatory effects of mussel (Aulacomya maoriana Iredale 1915) larval settlement by neuroactive compounds, amino acids and bacterial biofilms

Active Enzyme Nanocoatings Affect Settlement of Balanus amphitrite Barnacle Cyprids

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