Immunity to Salmonella Infection

Eisenstein, Toby K.; Sultzer, Barnet M.

doi:10.1007/978-1-4684-4481-0_26

Cited by 85 publications

(37 citation statements)

References 105 publications

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“…A previous study using polyclonal sera to Legionella pneumophila reported that passive immunization was protective in genetically resistant but not susceptible mouse strains (67). That result was interpreted as reflecting mouse strain-related differences in the macrophage efficacy for Ab-opsonized bacteria.…”

Section: Discussionmentioning

confidence: 96%

Mouse Genetic Background Is a Major Determinant of Isotype-Related Differences for Antibody-Mediated Protective Efficacy againstCryptococcus neoformans

Rivera

Casadevall

2005

The Journal of Immunology

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The protective efficacy of mAbs to Cryptococcus neoformans glucuronoxylomannan depends on Ab isotype. Previous studies in A/JCr and C57BL/6J mice showed relative protective efficacy of IgG1, IgG2a ≫ IgG3. However, we now report that in C57BL/6J × 129/Sv mice, IgG3 is protective while IgG1 is not protective, with neither isotype being protective in 129/Sv mice. IgG1, IgG2a, and IgG3 had different effects on IFN-γ expression in infected C57BL/6J × 129/Sv mice. IgG1-treated C57BL/6J × 129/Sv mice had significantly more pulmonary eosinophilia than IgG2a- and IgG3-treated C57BL/6J × 129/Sv mice. C. neoformans infection and Ab administration had different effects on FcγRI, FcγRII, and FcγRIII expression in C57BL/6J, 129/Sv, and C57BL/6J × 129/Sv mice. Our results indicate that the relative efficacy of Ab isotype function against C. neoformans is a function of the genetic background of the host and that IgG3-mediated protection in C57BL/6J × 129/Sv mice was associated with lower levels of IFN-γ and fewer pulmonary eosinophils. The dependence of isotype efficacy on host genetics underscores a previously unsuspected complex relationship between the cellular and humoral arms of the adaptive immune response.

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Section: Discussionmentioning

confidence: 96%

Mouse Genetic Background Is a Major Determinant of Isotype-Related Differences for Antibody-Mediated Protective Efficacy againstCryptococcus neoformans

Rivera

Casadevall

2005

The Journal of Immunology

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“…Natural or experimental infections of animals with Salmonella result in stimulation of both humoral and cell-mediated immunity (8,10). These immune responses primarily occur against the lipopolysaccharide (LPS) and major outer membrane (OM) proteins, including porins and OmpA (1,7,21,28,40,42).…”

mentioning

confidence: 99%

The C-Terminal Domain of Salmonella enterica Serovar Typhimurium OmpA Is an Immunodominant Antigen in Mice but Appears To Be Only Partially Exposed on the Bacterial Cell Surface

et al. 2003

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We examined the way the major outer membrane protein OmpA of Salmonella enterica serovar Typhimurium is recognized by the mouse immune system, by raising a panel of 12 monoclonal antibodies (MAbs) against this protein. Interaction between OmpA and these MAbs is competitively inhibited with several-hundredfold dilutions of mouse polyclonal sera obtained by immunization with live or heat-killed whole cells, suggesting that OmpA is one of the immunodominant antigens of serovar Typhimurium. All of the MAbs were specific for an identical epitope(s) located on the C-terminal domain of OmpA, as indicated by the use of OmpA fragments generated by protease or cyanogen bromide treatment and by competitive inhibition enzyme-linked immunosorbent assay. This epitope was highly conserved within (but not outside) the family Enterobacteriaceae. The strong immunogenicity of this epitope was surprising because the C-terminal domain of OmpA, usually thought to be located in the periplasm, is not expected to be exposed on the bacterial cell surface. A MAb, however, reacted in a cytofluorometry assay more strongly with outer-membrane-permeabilized cells than with untreated cells, a result supporting the predominantly periplasmic localization of the epitope. Significant, though low-level, reactivity of intact cells nevertheless suggests that in some cells the C-terminal domain of OmpA is exposed on the surface, a result consistent with the proposal that OmpA can fold into one of the two alternate conformations.

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“…The bacteria preferentially invade the mucosa through unique epithelial cells known as M cells which are found within the follicle-associated epithelium overlying mucosa-associated lymphoid tissue (5,17). Within the mucosa the bacteria are detected within both dendritic cells and macrophages (9,15). Alternatively, non-invasive strains of S. typhimurium, which do not invade M cells, can be transported directly by CD18 expressing phagocytes from the gastrointestinal tract into the systemic circulation as recently shown by Vazquez-Torres (36 vide an important line of defense against enteric pathogens, the interaction between Salmonella and these host cells may be critical for disease outcome (9, 1 1).…”

mentioning

confidence: 99%