Immunization Against Ovine Haemonchosis with Three Low Molecular Weight Somatic Antigens of Adult <i>Haemonchus contortus</i>

Alunda, José María; Angulo-Cubillán, Francisco; Cuquerella, Montserrat

doi:10.1046/j.1439-0450.2003.00611.x

Cited by 14 publications

(8 citation statements)

References 21 publications

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“…Another protein (known as Hc23), this time identified in somatic extracts of adult H. contortus as the most abundant component of a low molecular weight fraction that had protective potential, was purified and tested in sheep . In vaccine trials using two different adjuvants (aluminium hydroxide or E. coli lipopolysaccharide plus inactivated Propionibacterium acnes ), lambs that received the Hc23 antigen in either adjuvant had significantly lower FECs and lower worm burdens after challenge compared to challenge control lambs .…”

Section: Current Status Of Subunit Vaccines For the Main Gin Targets mentioning

confidence: 99%

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Matthews

Geldhof

Tzelos

et al. 2016

Parasite Immunology

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SummaryThe global increase in anthelmintic resistant nematodes of ruminants, together with consumer concerns about chemicals in food, necessitates the development of alternative methods of control for these pathogens. Subunit recombinant vaccines are ideally placed to fill this gap. Indeed, they are probably the only valid option for the long-term control of ruminant parasitic nematodes given the increasing ubiquity of multidrug resistance in a range of worm species across the world. The development of a subunit multicellular parasite vaccine to the point of practical application would be a groundbreaking step in the control of these important endemic infections of livestock. This review summarizes the current status of subunit vaccine development for a number of important gastrointestinal nematodes of cattle and sheep, with a focus on the limitations and problems encountered thus far, and suggestions as to how these hurdles might be overcome. K E Y W O R D Sgastrointestinal nematodes, ruminants, vaccines

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Section: Current Status Of Subunit Vaccines For the Main Gin Targets mentioning

confidence: 99%

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Matthews

Geldhof

Tzelos

et al. 2016

Parasite Immunology

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“…In addition, large numbers of adult nematodes are necessary to extract limited amounts of natural antigen; therefore, this will only be practical when methods are derived to artificially make the antigen through recombinant technology so that it can be mass-produced at a lower cost. Vaccines for other nematodes that do not feed on blood have largely focused on using antigens found in worm somatic tissue and secretory/excretory products (Griffiths and Prichard, 1994;Schallig and Van Leeuwen, 1997;Emery et al, 1999;Alunda et al, 2003). These antigens are usually recognized by the host after infection; thus, vaccine immunity should be obtained by natural exposure.…”

Section: Vaccinesmentioning

confidence: 99%

Immunological aspects of nematode parasite control in sheep1

Miller

Horohov

2006

Journal of Animal Science

137

102

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Gastrointestinal nematode parasitism is arguably the most serious constraint affecting sheep production worldwide. Economic losses are caused by decreased production, the costs of prophylaxis and treatment, and the death of the infected animals. The nematode of particular concern is Haemonchus contortus, which can cause severe blood loss resulting in anemia, anorexia, depression, loss of condition, and eventual death. The control of nematode parasites traditionally relies on anthelmintic treatment. The evolution of anthelmintic resistance in nematode populations threatens the success of drug treatment programs. Alternative strategies for control of nematode infections are being developed, and one approach is to take advantage of the host's natural or acquired immune responses, which can be used in selection programs to increase the level of resistance in the population. Vaccination can also be used to stimulate or boost the host's acquired immunity. The induction of protective resistance is dependent on the pattern of cytokine gene expression induced during infection by two defined CD4+ T-helper cell subsets, which have been designated as Th1 or Th2. Intracellular parasites most often invoke a Th1-type response, and helminth parasites a Th2-type response. Breeds of sheep resistant to infection have developed resistance over a much longer term of host-parasite relationship than genetically selected resistant lines. The immune components involved in these different responses and types of host-parasite relationships will be reviewed. The potential for using vaccines has been investigated, with variable results, for several decades. The few successes and potential new antigen candidates will also be reviewed.

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“…Anti-F4, anti-F8 and, anti-F14 were pooled sera from a lamb immunization experiment carried out earlier with isolated protein fractions of adult H. contortus soluble extracts (Alunda et al 2003). Anti-p26/23 lamb serum was obtained from one of the animals employed in a vaccination experiment with p26/23 of H. contortus (Domínguez-Toraño et al 2000).…”

Section: Antigens and Seramentioning

confidence: 99%

Immunobiological characterization of Graphidium strigosum experimental infection in rabbits (Oryctolagus cuniculus)

Cuquerella

Alunda

2008

Parasitol Res

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An experimental infection of rabbits with a wild isolate of the gastric nematode Graphidium strigosum was carried out. Animals (3.5 months age) were infected with 1,000 L3 administered by bucoesophagic catheter (five rabbits) or kept as uninfected control group (five animals). The infection was maintained for 3 months. Along the experimental period, some parasitological, hematological and immunological parameters were determined. Prepatent period of the infection ranged from 30 to 38 days and, at necropsy, average adult helminth counts were 430.75 +/- 126.12. No significant variations were found in packed cell volume, leukocyte, and eosinophil counts along the experimental period. Infection elicited a clear serum-specific IgG response, estimated by ELISA, during patency. Pooled sera from the patent period of the infection recognized some soluble antigens, particularly, a 67-kDa protein. Experimentally infected animals did not show cross recognition between G. strigosum, Haemonchus contortus, and Teladorsagia circumcincta. However, Western blot analysis with hyperimmune sera against H. contortus raised in rabbits and lambs showed cross reactivity between this helminth species and G. strigosum.

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Immunization Against Ovine Haemonchosis with Three Low Molecular Weight Somatic Antigens of Adult Haemonchus contortus

Cited by 14 publications

References 21 publications

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Progress in the development of subunit vaccines for gastrointestinal nematodes of ruminants

Immunological aspects of nematode parasite control in sheep1

Immunobiological characterization of Graphidium strigosum experimental infection in rabbits (Oryctolagus cuniculus)

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