Immunization by influenza virus-like particles protects aged mice against lethal influenza virus challenge

Wen, Zhiyuan; Ye, Ling; Gao, Yulong; Pan, Lei; Dong, Ke; Bu, Zhigao; Compans, Richard W.; Yang, Chinglai

doi:10.1016/j.antiviral.2009.09.005

Cited by 38 publications

(35 citation statements)

References 56 publications

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“…These approaches include expression in bacteria [37][38][39], expression in insect cells through the use of baculovirus expression vectors [40][41][42][43], and expression in mammalian cells either through the use of virus vectors [44][45][46][47] or standard mammalian expression vectors [12][13][14][15][16][48][49][50].…”

Section: Discussionmentioning

confidence: 99%

Recombinant respiratory syncytial virus F protein expression is hindered by inefficient nuclear export and mRNA processing

Huang¹,

Lawlor²,

Tang³

et al. 2010

Virus Genes

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Studies of the fusion activity of respiratory syncytial virus (RSV) F protein are significantly hindered by low recombinant expression levels. While infection produces F protein levels detectable by western blot, recombinant expression produces undetectable to low levels of F protein. Identifying the obstacles that hinder recombinant F protein expression may lead to improved expression and facilitate the study of F protein function. We hypothesized that nuclear localization and/or inefficient RNA polymerase II-mediated transcription contribute to poor recombinant F protein expression. This study shows a combination of stalled nuclear export, premature polyadenylation, and low mRNA abundance all contribute to low recombinant F protein expression levels. In addition, this study provides an expression optimization strategy that results in greater F protein expression levels than observed by codon-optimization of the F protein gene, which will be useful for future studies of F protein function.

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Section: Discussionmentioning

confidence: 99%

Recombinant respiratory syncytial virus F protein expression is hindered by inefficient nuclear export and mRNA processing

Huang¹,

Lawlor²,

Tang³

et al. 2010

Virus Genes

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“…Anti-influenza hemagglutination-specific antibodies in serum were measured by ELISA following a protocol that has been previously described (27). The purified hemagglutination protein (2 mg/L) was used to coat ELISA plates.…”

Section: Methodsmentioning

confidence: 99%

Dietary Wolfberry Supplementation Enhances the Protective Effect of Flu Vaccine against Influenza Challenge in Aged Mice

Wang

Niu

et al. 2014

The Journal of Nutrition

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Current vaccines for influenza do not fully protect the aged against influenza infection. Although wolfberry (goji berry) has been shown to improve immune response, including enhanced antibody production, after vaccination in the aged, it is not known if this effect would translate to better protection after influenza infection, nor is its underlying mechanism well understood. To address these issues, we conducted a study using a 2 × 2 design in which aged male mice (20-22 mo) were fed a control or a 5% wolfberry diet for 30 d, then immunized with an influenza vaccine or saline (control) on days 31 and 52 of the dietary intervention, and finally challenged with influenza A/Puerto Rico/8/34 virus. Mice fed wolfberry had higher influenza antibody titers and improved symptoms (less postinfection weight loss) compared with the mice treated by vaccine alone. Furthermore, an in vitro mechanistic study showed that wolfberry supplementation enhanced maturation and activity of antigen-presenting dendritic cells (DCs) in aged mice, as indicated by phenotypic change in expression of DC activation markers major histocompatibility complex class II, cluster of differentiation (CD) 40, CD80, and CD86, and functional change in DC production of cytokines interleukin-12 and tumor necrosis factor-α as well as DC endocytosis. Also, adoptive transfer of wolfberry-treated bone marrow DCs (loaded with ovalbumin(323-339)-peptide) promoted antigen-specific T cell proliferation as well as interleukin-4 and interferon-γ production in CD4(+) T cells. In summary, our data indicate that dietary wolfberry enhances the efficacy of influenza vaccination, resulting in better host protection to prevent subsequent influenza infection; this effect may be partly attributed to improved DC function.

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“…Altogether, results from these studies suggest that regulatory T cells may play an important role in regulating activation of immune responses in aged animals and elderly humans. We have previously shown that immunization with influenza virus-like particles (VLPs) or inactivated influenza virus vaccines (IIV) induced significantly lower levels of antibody responses in aged mice than in young adult mice [35]. Further, it was observed that a higher vaccine dose is required to confer a complete protection against influenza virus infection in the aged mice.…”

Section: Introductionmentioning

confidence: 99%

Blockage of regulatory T cells augments induction of protective immune responses by influenza virus-like particles in aged mice

Wen

Wang

Dong

et al. 2017

Microbes and Infection

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Elderly humans over 65 years old are at great risk to pathogenesis by influenza virus infection. However, although influenza vaccines provide effective protection in healthy young adults, protection of elderly adults is substantially lower even with a good match between the vaccine and the circulating influenza virus. To gain insight of the underlying mechanism for the reduced immunogenicity of influenza vaccines in the aged population, we investigated immunogenicity of influenza virus-like particle vaccines in aged mice, which represent a useful model for studying aging associated impairment in immune responses. Specifically, we investigated the effect of inhibiting regulatory T cells in aged mice on induction of protective immune responses by influenza vaccines. Our results showed that injecting anti-CD25 antibodies could down-regulate CD25 on the surface of regulatory T cells and significantly increase the levels of antibody responses induced by VLP immunization in aged mice. Further, the profiles of antibody responses were also changed towards Th1 type by regulatory T cell blockage in aged mice. Moreover, aged mice that were treated by anti-CD25 antibodies prior to vaccination were more effectively protected against lethal influenza virus challenge.

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Immunization by influenza virus-like particles protects aged mice against lethal influenza virus challenge

Cited by 38 publications

References 56 publications

Recombinant respiratory syncytial virus F protein expression is hindered by inefficient nuclear export and mRNA processing

Recombinant respiratory syncytial virus F protein expression is hindered by inefficient nuclear export and mRNA processing

Dietary Wolfberry Supplementation Enhances the Protective Effect of Flu Vaccine against Influenza Challenge in Aged Mice

Blockage of regulatory T cells augments induction of protective immune responses by influenza virus-like particles in aged mice

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