Immunization of cattle against Theileria parva infection using recombinant vaccinia viruses expressing a 67 kDa sporozoite surface antigen and bovine cytokines

Honda, Yoko; Waithaka, M; Taracha, E.L.N.; Duchateau, Luc; Musoke, A.J.; McKeever, Declan J.

doi:10.1016/s1383-5769(98)81090-8

Cited by 1 publication

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“…Consequently, IL-2 production is critical for the generation of both humoral and cell-mediated immunity, and these characteristics have led to the assessment of IL-2 as a vaccine adjuvant in several animal models. The potential adjuvant effects of IL-2 have been demonstrated by its capacity to augment immune responses in vaccine model systems of cattle, (13)(14)(15)(16) guinea pigs, (17) pigs, (18,19) and mice. (20)(21)(22)(23) Although the adjuvant potential of IL-2 in mammalian systems has been studied widely, little work has been performed on nonmammalian systems to date.…”

Section: Introductionmentioning

confidence: 99%

Interleukin-2 Directly Induces Activation and Proliferation of Chicken T CellsIn Vivo

Hilton

Bean

Kimpton

et al. 2002

Journal of Interferon & Cytokine Research

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Cytokines, as immune activators, have been investigated in mammalian systems as natural adjuvants and therapeutics. In particular, interleukin-2 (IL-2) has been studied widely as a vaccine adjuvant and immuno-enhancer because of its role in activating T cell proliferation. We show here that the first nonmammalian IL-2 gene cloned, chicken IL-2 (ChIL-2), exhibits similar biologic activities to those of mammalian IL-2. To assess the activities of ChIL-2 in vivo, we injected birds with recombinant ChIL-2 (rChIL-2) protein. rChIL-2 treatment induced peripheral blood lymphocytes to express cell surface IL-2 receptors (IL-2R) within 48 h and resulted in an increase in the proportion of peripheral blood CD4+ and CD8+ T cells. Using bromodeoxyuridine (BrdU) incorporation as a measurement of cell proliferation, we showed the increase in T cell populations to be due to cell proliferation. The ability of ChIL-2 to cause both activation and proliferation of T cells in vivo indicates that it has the potential to be used as an immune activator.

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