Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum

İspir, Ünal; Türk, Cebrahil; Kırıcı, Muammer; Taysi, Mehmet Reşit; Özcan, Mikail

doi:10.12714/egejfas.2016.33.3.07

Cited by 2 publications

(1 citation statement)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10.11113/mjfas.v20n1.3187 The higher survival rate in the current study compared to previous studies [2,25,42,43,48,79] may be attributed to different circumstances like capsule size, applied biopolymers, type of vaccine and vaccine concentration within the capsules, capsules fabrication method, fish species, duration of immunization, pathogen type in addition to dose and timing of challenge after vaccination.…”

Section: Relative Percent Of Survival Rps After 14 Day Of Ip Challengecontrasting

confidence: 50%

The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules

M Abu Darwish,

Rozaini,

Sheikh

et al. 2024

Mal. J. Fund. Appl. Sci.

View full text Add to dashboard Cite

Nile tilapia is one of the major farmed fish that is often infected with Streptococcus. Oral vaccination is the most preferable technique, but the antigen is usually degraded by gastric juice. Encapsulation by alginate could be effective in protecting the antigen. The aim of this study was to assess the effectiveness of vaccinating Nile tilapia using formalin-killed Streptococcus agalactiae encapsulated in alginate. The average diameter of fabricated microcapsules was 500±20 μm, with 86% encapsulation efficacy. Fish were then divided into four groups (30 fish per group): group A: fish vaccinated by alginate microcapsules covered by formalin-killed cells (FKC), group B: fish vaccinated by non-covered FKC, group C: fish fed with empty alginate microcapsules and group D: fish feed with commercial pellets as control group. All groups were treated for 14 days and then fed with commercial pellets. Blood samples were collected on days 7, 14, 21, and 28 days post-vaccination. Bactericidal activity, lysozyme activity and serum antibody titer were significantly elevated in group A (p<0.001) compared to other treated groups. Then, gene expression analysis was performed at 29 days post-vaccination to evaluate the expression of main immune contributors such IgM, IgT, TCR β, CD4, CD8α, IL 1β, IL 8, IFN 1, and TNFα. The analysis showed significant upregulation of all tested genes in group A compared to other treated groups (p<0.001). Lastly, a challenge test was done on day 30 post-vaccination by injecting 4.6×106 CFU mL−1 of virulent S. agalactiae. The relative percent of survival (RPS) were 92± 2%, 48± 5% and 4± 3% for groups A, B and C respectively. The obtained result indicated that alginate encapsulation provided antigen protection due to its higher immunogenicity compared to non-covered vaccine. Hence, the fabricated vaccine could be incorporated with food and orally administered to Nile tilapia to prevent S. agalactiae infection.

show abstract

Section: Relative Percent Of Survival Rps After 14 Day Of Ip Challengecontrasting

confidence: 50%

The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules

M Abu Darwish,

Rozaini,

Sheikh

et al. 2024

Mal. J. Fund. Appl. Sci.

View full text Add to dashboard Cite

show abstract

Formalin ile İnaktive Flavobacterium psychrophilum’un Gökkuşağı Alabalığı (Oncorhynchus mykkiss)’nın Antioksidan Enzimlerine Etkisi

Kırıcı

Taysi

et al. 2021

Türk Tarım Ve Doğa Bilimleri Dergisi

View full text Add to dashboard Cite

Bu çalışmada, formalin ile inaktive edilen Flavobacterium psychrophilum'un yavru gökkuşağı alabalıklarında bazı antioksidan enzim parametrelerinde oluşturduğu değişiklikler incelenmiştir. Yaklaşık 1.0 g ağırlığında olan gökkuşağı alabalığı yavruları formalin ile inaktive edilmiş 1x108 CFU/ml bakterin ile 30, 60 ve 90 dakika muamele edildi. Balıklar, uygulamadan 21 gün sonra 1x108 CFU/ml canlı F. psychrophilum ile eprüvasyon yapıldı. On dört gün sonra ise balıklardan alınan örnekler ile malondialdehit (MDA), süperoksit dismutaz, katalaz ve redükte glutatyon aktivitelerindeki değişiklikler incelendi. MDA düzeyinde özelikle 60 ve 90 dak. bakteri uygulanan balıklarda ılımlı pozitif kontrol grubuna göre bir azalış gözlendi. Antioksidan enzim aktivitelerinde de aynı şekilde olumlu yönde bir değişiklik saptandı. Sonuç olarak; formalin ile inaktive edilen bakteri preparatının, canlı F. psychrophilum bakterisi tarafından bozulan oksidan/antioksidan dengesinin düzenlenmesinde etkili olabileceği tespit edilmiştir.

show abstract

Immunization of rainbow trout (Oncorhynchus mykiss) with alginate microparticles containing inactivated Flavobacterium psychrophilum

Cited by 2 publications

References 3 publications

The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules

The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules

Formalin ile İnaktive Flavobacterium psychrophilum’un Gökkuşağı Alabalığı (Oncorhynchus mykkiss)’nın Antioksidan Enzimlerine Etkisi

Contact Info

Product

Resources

About