Immunization with the biologically active lectin domain of PapGII induces strong adhesion-inhibiting antibody responses but not protection against avian pathogenic<i>Escherichia coli</i>

Vandemaele, Fréderic; Bleyen, Nele; Abuaboud, Omran; vandermeer, Ed; Jacobs, A. A. C.; Goddeeris, Bruno

doi:10.1080/03079450600710997

Cited by 18 publications

(9 citation statements)

References 34 publications

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“…However, intramuscular vaccination of mice and monkeys with recombinant minor adhesive FimH subunits protects them against uropathogenic E. coli challenge (Langermann et al, 1997(Langermann et al, , 2000. In addition, intramuscular vaccination of chickens with the minor P pilus subunit and adhesin PapGII induced antibodies that could inhibit avian pathogenic E. coli binding (Vandemaele et al, 2006). Recently, oral immunization with recombinant adhesins is reported to induce a partial protection (Seo et al, 2002;Verdonck et al, 2004Verdonck et al, , 2005.…”

Section: Discussionmentioning

confidence: 99%

Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Verdonck

Tiels

Gog

et al. 2007

Veterinary Immunology and Immunopathology

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Post-weaning diarrhoea and oedema disease in weaned piglets are caused by infection with F4 + or F18 + Escherichia coli strains. There is no commercial vaccine available, but it is shown that oral immunization of weaned piglets with purified F4 fimbriae induces a protective mucosal immune response. In the present study, piglets were orally and nasally immunized with purified F18 fimbriae in the presence of the mucosal adjuvant LT(R192G) or CTA1-DD, respectively. This immunization could not lead to protection against F18 + E. coli infection. The induced F18-specific immune response was directed towards the major subunit FedA and weakly towards the adhesive subunit FedF. The results of these experiments demonstrate that it is difficult to induce protective immunity against F18+ E. coli using the whole fimbriae due to the low response against the adhesin. #

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Section: Discussionmentioning

confidence: 99%

Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Verdonck

Tiels

Gog

et al. 2007

Veterinary Immunology and Immunopathology

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“…Early attempts in APEC vaccines resulted only in protection against homologous challenge; that is, protection was only effective against the single strain that was used to create the vaccine [ 152 , 161 , 162 , 163 ]. Effective vaccination whether by subunit vaccines, that target specific genetic virulence factors, or by live attenuated vaccines (LAV), have mostly resulted in coverage only against homologous challenge [ 164 , 165 , 166 , 167 ]. One commercially available LAV produced from nonpathogenic E. coli provides protection via cell-mediated immunity [ 168 ].…”

Section: Disease Prevention Strategies In Naementioning

confidence: 99%

Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in No Antibiotics Ever Broiler Production and Potential Solutions

et al. 2020

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United States is the largest producer and the second largest exporter of broiler meat in the world. In the US, broiler production is largely converting to antibiotic-free programs which has caused an increase in morbidity and mortality within broiler farms. Escherichia coli and Clostridium perfringens are two important pathogenic bacteria readily found in the broiler environment and result in annual billion-dollar losses from colibacillosis, gangrenous dermatitis, and necrotic enteritis. The broiler industry is in search of non-antibiotic alternatives including novel vaccines, prebiotics, probiotics, and housing management strategies to mitigate production losses due to these diseases. This review provides an overview of the broiler industry and antibiotic free production, current challenges, and emerging research on antibiotic alternatives to reduce pathogenic microbial presence and improve bird health.

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“…Significant differences were found only with the ECP-positive strain APEC-O2; no significant differences were found with ECP-negative χ7122. Other studies reported failure of APEC vaccines to protect against homologous and/or heterologous challenge in poultry ( 60 , 62 , 68 , 69 , 79 ). In the current study, the lack of strong protection after vaccination may be due to the route of infection.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Spleen Transcriptome and Immunity Against Avian Colibacillosis After Immunization With Recombinant Attenuated Salmonella Vaccine Strains

et al. 2018

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Avian pathogenic Escherichia coli (APEC) causes extraintestinal infections in poultry. Vaccines targeting APEC in chickens have been partially successful, but many lack heterologous protection. Recombinant attenuated Salmonella vaccine (RASV) strains can induce broad immunity against Salmonella and be modified to deliver E. coli antigens. Along with vaccine characteristics, understanding the host response is crucial for developing improved vaccines. The objectives of this study were to evaluate host responses to vaccination with an RASV producing E. coli common pilus (ECP) and assess protection against APEC infection in chickens. Four-day-old White Leghorn chickens were unvaccinated or orally vaccinated and boosted 2 weeks later with RASV χ8025(pYA3337), RASV χ8025(pYA4428) carrying ecp operon genes, or a combination of χ8025(pYA3337) and χ8025(pYA4428) (Combo). To assess host responses, serum IgY and intestinal IgA antibody titers were measured, and spleen samples (n = 4/group) were collected from unvaccinated and Combo vaccinated 4-week-old chickens for RNA-seq. Vaccine protection potential against Salmonella and APEC was evaluated in vitro using bacterial inhibition assays. Five-week-old chickens were challenged via air sac with either an APEC O2 or O78 strain. E. coli was enumerated from internal organs, and gross colibacillosis lesions were scored at necropsy. RASV immunized chickens elicited anti-E. coli antibodies. The spleen transcriptome revealed that 93% (89/96) of differentially expressed genes (DEG) were more highly expressed in Combo vaccinated compared to unvaccinated chickens, with signal as the most significantly impacted category. RNA-seq analysis also revealed altered cellular and metabolic processes, response to stimulus after vaccination, and immune system processes. Six DEG including genes linked to transcription regulation, actin cytoskeleton, and signaling were highly positively correlated with antibody levels. Samples from RASV immunized chickens showed protection potential against Salmonella strains using in vitro assays, but a variable response was found for APEC strains. After APEC challenges, significant differences were not detected for bacterial loads or gross lesions scores, but χ8025(pYA3337) immunized and χ8025(pYA4428) immunized chickens had significantly fewer number of APEC-O2-positive samples than unvaccinated chickens. This study shows that RASVs can prime the immune system for APEC infection, and is a first step toward developing improved therapeutics for APEC infections in chickens.

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Immunization with the biologically active lectin domain of PapGII induces strong adhesion-inhibiting antibody responses but not protection against avian pathogenicEscherichia coli

Cited by 18 publications

References 34 publications

Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection

Avian Pathogenic Escherichia coli and Clostridium perfringens: Challenges in No Antibiotics Ever Broiler Production and Potential Solutions

Characterization of Spleen Transcriptome and Immunity Against Avian Colibacillosis After Immunization With Recombinant Attenuated Salmonella Vaccine Strains

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