Immuno-diagnosis of bubaline fasciolosis with Fasciola gigantica cathepsin-L and recombinant cathepsin L 1-D proteases

Raina, O.K.; Yadav, Shobha; Sriveny, D.; Gupta, S. C.

doi:10.1016/j.actatropica.2006.03.004

Cited by 41 publications

(34 citation statements)

References 20 publications

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“…The FgCL-3 ELISA showed 100 and 97% sensitivity under experimental and field situations, respectively in the diagnosis of the disease. The infection could be detected as early as two wpi (Dixit et al 2004;Raina et al 2006). The FgCL-3 was also evaluated for its potential in the detection of early prepatent infection in experimentally infected bovine calves infected with F. gigantica infection.…”

Section: Diagnosismentioning

confidence: 94%

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Sharma¹,

Godara²,

Thilagar³

2011

J Parasit Dis

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On the Indian sub-continent, nearly 5,000 years ago, the domestication of the riverine buffalo-the incredible Asian dairy animal was initiated. It plays a versatile role in socio-economic upliftment of its owners from the rural agricultural communities in Asian, African, South American and a few European countries. Comparatively, buffaloes are lesser evolved and susceptible to infectious diseases than cattle. However, poor body thermoregulation and wallowing nature predisposed them to snail borne infections, especially tropical fasciolosis-an incessant major constraint on buffalo production and improvement programmes. This review article is an insight into the global prevalence, varied epizootiological factors, offers possible explanation to pathophysiological clinical signs, deleterious effects of the tropical liver fluke, involving hepato-biliary system, haemopoitic system, endocrine glands and their secretions, oxidative stress, altered metabolism and significant fall in food conversion efficiency with unaffected digestibility of nutrients. Besides, the authors have briefly discussed and reviewed the developments and significance of successful immunodiagnostic approaches for detecting and forecasting the disease during early pre-patency and feasibility of developing a cost effective immunoprotection strategies against tropical fasciolosis.

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Section: Diagnosismentioning

confidence: 94%

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Sharma¹,

Godara²,

Thilagar³

2011

J Parasit Dis

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“…In our study, only 2 (3.3%) true-positive cases (positive in CE) fell below the cutoff value with FhFtn-1, whereas 5 (8.3%) truepositive cases were recorded as negative with FhTP16.5. Since all serum samples were properly preserved at Ϫ70°C from their collection until use, it is possible to speculate that the sensitivities of the two assays were influenced by factors such as the intensity of infection, the persistence of antibodies postinfection, and parasite senescence (21). Northern Peru and Bolivian Altiplano are two regions in which human fascioliasis is highly endemic (3,6,(43)(44)(45)(46).…”

Section: Discussionmentioning

confidence: 99%

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

et al. 2014

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Coprological examination based on egg detection in stool samples is currently used as the gold standard for the diagnosis of human fascioliasis. However, this method is not effective during the acute phase of the disease and has poor sensitivity during the chronic phase. Serodiagnosis has become an excellent alternative to coprological examination in efforts to combat the effects of fascioliasis on human and animal health. Two novel recombinant Fasciola hepatica proteins, i.e., a ferritin (FhFtn-1) and a tegument-associated protein (FhTP16.5), were used as antigens to develop in-house enzyme-linked immunosorbent assay (ELISA) methods. The assays were optimized and validated using 152 serum samples from humans with a known infection status, including healthy subjects, patients with chronic fascioliasis, and patients with other parasitic diseases. The FhFtn-1 ELISA was shown to be 96.6% sensitive and 95.7% specific; the respective parameters for the FhTP16.5 ELISA were 91.4% and 92.4%. The performances of the FhFtn-1 and FhTP16.5 ELISAs were compared with that of an available commercial test (the DRG test) using a subset of serum samples. Our in-house tests were slightly more sensitive than the DRG test in detecting antibodies against F. hepatica, but the differences were not statistically significant. In conclusion, the present study provides evidence for the potential of the FhFtn-1 and FhTP16.5 ELISAs as diagnostic tools for human fascioliasis, as might be implemented in conjunction with standard assays for large-scale screenings in areas where the disease is endemic and for the detection of occasional cases in clinical laboratories.

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“…[4][5][6][24][25][26] Cornelissen et al 5 documented the high specificity and sensitivity of peptide based on cathepsin-L amino acids 110-129 in ELISA detection of anti-F. hepatica antibodies in calves. We have herein extended this study by examining sheep fasciolosis immunodiagnostic potential of three peptide constructs, based on the amino terminal region of F. gigantica cathepsin L enzymatic site (amino acids 110-123).…”

Section: Discussionmentioning

confidence: 99%

“…These data support the data documenting the high specificity of cathepsin L proteinase in Fasciola immunodiagnosis. [4][5][6][24][25][26] However, antibodies against the different peptide constructs were not detectable by ELISA until 4 weeks of infection. Additionally, the sensitivity of the peptide-based ELISA was greatly improved by using anti whole IgG rather than an IgG-specific, conjugate.…”

Section: Discussionmentioning

confidence: 99%

Fasciola gigantica cathepsin L proteinase‐based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis

et al. 2008

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Immuno-diagnosis of bubaline fasciolosis with Fasciola gigantica cathepsin-L and recombinant cathepsin L 1-D proteases

Cited by 41 publications

References 20 publications

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Epizootiology, pathogenesis and immunoprophylactic trends to control tropical bubaline fasciolosis: an overview

Development of Two Antibody Detection Enzyme-Linked Immunosorbent Assays for Serodiagnosis of Human Chronic Fascioliasis

Fasciola gigantica cathepsin L proteinase‐based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis

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