Immuno-Modulatory Activities of Polysaccharides Separated from Jubak in Macrophage Cells

Protaetia brevitarsis seulensis (P. brevitarsis) larvae have traditionally been used in alternative medicine. Although various health benefits have been reported, the immunomodulatory effects of P. brevitarsis extract have so far been unknown. In this study, the immune-enhancing activities of P. brevitarsis larvae hot-water extract (PLW) were investigated using the RAW 264.7 macrophage cell line. The PLW did not exert cytotoxicity at concentrations ranging from 1 to 200 μg/mL in RAW 264.7 cells. The treatment of PLW increased the production of nitric oxide and cytokines [tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6)] at doses of 100, 200 μg/mL in RAW 264.7 cells. Additionally, treatment with PLW (100, 200 μg/mL) led to the increase of surface molecules (cluster of differentiation; CD80/86 and major histocompatibility complex; MHC-class Ⅰ/Ⅱ) expression in RAW 264.7 cells. These immunomodulatory effects of PLW were mediated by mitogen-activated protein kinases (MAPK-extracellular signal-regulated kinase, c-Jun N-terminal kinase, p38 MAPK) phosphorylation and nuclear factor (NF)-κB translocation. In conclusion, these findings provide experimental evidence that PLW can be used as an immunity-enhancing nutraceutical ingredient.

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Immuno-Modulatory Activities of Polysaccharides Separated from Jubak in Macrophage Cells

Cited by 7 publications

References 14 publications

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Immuno-Modulatory Activity of Hot Water Extracts Isolated from Protaetia brevitarsis seulensis Larvae through MAPKs and NF-κB Pathways in Macrophages

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