Immuno‐regulatory cytokines in asthma: IL‐15 and IL‐13 in induced sputum

Komai-Koma, Mousa; McKay, Anne; Thomson, L.; McSharry, Charles; Chalmers, George; Liew, F. Y.; Thomson, Neil C.

doi:10.1046/j.1365-2222.2001.01174.x

Cited by 37 publications

(37 citation statements)

References 25 publications

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“…The present authors cultured sputum inflammatory cells of asthmatic and normal subjects. In the few studies that tried to keep sputum cells in culture to evaluate the inflammatory characteristics of asthma, sputum cells were cultured without fractionation [18][19][20][21][22]. The cells were separated into two fractions, adherent and nonadherent, and cytokines, chemokines and cell composition were evaluated in each fraction.…”

Section: Discussionmentioning

confidence: 99%

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Eotaxin-2 in sputum cell culture to evaluate asthma inflammation

Scheicher¹,

Teixeira²,

Cunha³

et al. 2006

Eur Respir J

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The aim of the present study was to elucidate whether the culture of cells recovered from induced sputum may represent a suitable model to evaluate cytokine and chemokine production by airway inflammatory cells.Sputum induction was performed in 21 normal subjects and 30 asthmatic patients. A total of 21 out of the 30 asthmatic patients were taking inhaled corticosteroids, while the remaining nine were steroid-naive asthmatics. The steroid-naive group was evaluated before and after a 14-day treatment with oral prednisone (40 mg?day -1). The supernatant of lysed and centrifuged sputum and the supernatant of sputum cell culture were analysed. Tumour necrosis factor-a, interleukin (IL)-8 (CXCL8), IL-1b, IL-13 and eotaxin-2 (CCL24) concentrations were determined by specific ELISA.Eotaxin-2 production by cell culture was higher in the asthma group (131¡108 pg?mL) than in the control group (36¡41 pg?mL -1 ) and treatment with oral corticosteroids eliminated this difference. In addition, reduction of eotaxin-2 levels by corticosteroid treatment was greater in cell culture (81.3% reduction) than in sputum (26.4%). There was correlation between the decrease in eotaxin-2 production and the decrease in blood eosinophil number and between eotaxin-2 and eosinophils in sputum.Eotaxin-2 may play an important role in asthma and the response to corticosteroid treatment suggests that analysis of sputum cell culture is relevant as an inflammatory parameter.

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Section: Discussionmentioning

confidence: 99%

“…Several studies [15][16][17] have used the culture of blood cells to examine asthma pathophysiology and the responsiveness to treatment. However, very few studies have employed the culture of sputum cells [18][19][20][21][22]. It is likely that cells from the airways provide better information due to their proximity to the site of asthmatic inflammation.…”

mentioning

confidence: 99%

Eotaxin-2 in sputum cell culture to evaluate asthma inflammation

Scheicher¹,

Teixeira²,

Cunha³

et al. 2006

Eur Respir J

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“…It is possible that in asthmatic patients, an increase in TGF-β1 expression may increase IL-15 expression, causing a shift of the Th1/Th2 balance in favour of Th1. This assumption is supported by the results of studies implying that the progress of asthma is accompanied by a weaker response of patients to antihistamines and antileukotrienes; reports on the activity of omalizumab in IgE non-dependent asthma (48) A previous study revealed significantly higher levels of IL-15 in the induced sputum of patients taking GCs compared with patients who had not been administered any therapy or normal controls (23). Also, the in vitro culture of sputum white cells, isolated from asthmatic patients treated with GCs, showed significantly higher levels of IL-15 synthesis in comparison with the control group (23).…”

Section: Discussionmentioning

confidence: 74%

“…This assumption is supported by the results of studies implying that the progress of asthma is accompanied by a weaker response of patients to antihistamines and antileukotrienes; reports on the activity of omalizumab in IgE non-dependent asthma (48) A previous study revealed significantly higher levels of IL-15 in the induced sputum of patients taking GCs compared with patients who had not been administered any therapy or normal controls (23). Also, the in vitro culture of sputum white cells, isolated from asthmatic patients treated with GCs, showed significantly higher levels of IL-15 synthesis in comparison with the control group (23). These results highlight the considerable immunomodulating role of IL-15 in asthma, which involves the induction of changes in the inflammation profile, from a profile connected with Th2 lymphocytes to one connected with Th1 lymphocytes.…”

Section: Discussionmentioning

confidence: 74%

“…Experiments on cell lines and the examination of bronchoalveolar lavage fluid have revealed that IL-15 expression in macrophages is increased in Rhinovirus infections, and that this mechanism is impaired in asthmatic patients (22). Moreover, IL-15 expression measured in induced expectorated matter and sputum cell culture has been shown to increase following the administration of glucocorticoid therapy (23).…”

Section: Introductionmentioning

confidence: 99%

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Role of IL‑15 in the modulation of TGF‑β1‑mediated inflammation in asthma

et al. 2017

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Abstract. Transforming growth factor (TGF)-β1 has an essential role in bronchitis and the induction of bronchial remodelling, which are critical processes in the pathogenesis of asthma. However, the role of interleukin (IL)-15 in asthma inflammation remains unclear. The aim of the present study was to evaluate the effect of TGF-β1 mRNA expression on IL-15 mRNA expression in asthmatic patients and to assess the role of IL-15 in the clinical course of asthma. The study included 221 participants, comprising 130 patients with asthma and 91 healthy volunteers. The participants were subjected to testing using spirometry, as well as the Asthma Control Test™ and Borg Scale. The expression of TGF-β1 and IL-15 mRNA was analyzed in blood samples using reverse transcription-quantitative polymerase chain reaction. Statistical analysis indicated that IL-15 and TGF-β1 mRNA expression each differed significantly between the patient and control groups (P= 0.0016 and P= 0.033, respectively). A significant correlation was identified between IL-15 expression and TGF-β1 expression (R=0.41, P=0.0005). No correlation was observed between IL-15 expression and the degree of asthma severity, the results of spirometric examination or the frequency of asthma exacerbations. Further analysis revealed that IL-15 expression was elevated following the administration of inhaled glucocorticosteroids (iGCs; P=0.024), and reduced following methylxanthine treatment (P<0.001). The occurrence of dyspnoea differed between the study and control groups, and this was not found to be associated with IL-15 expression. Since IL-15 expression was correlated with TGF-β1 expression among asthmatic patients, and IL-15 expression was elevated following iGC administration, the results of the study suggest that IL-15 activity might be associated with the pathogenesis of asthma.

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Interleukin 15, Interleukin 17, and Interleukin 25

Nakajima

Iwamoto

2011

Inflammation and Allergy Drug Design

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Immuno‐regulatory cytokines in asthma: IL‐15 and IL‐13 in induced sputum

Cited by 37 publications

References 25 publications

Eotaxin-2 in sputum cell culture to evaluate asthma inflammation

Eotaxin-2 in sputum cell culture to evaluate asthma inflammation

Role of IL‑15 in the modulation of TGF‑β1‑mediated inflammation in asthma

Interleukin 15, Interleukin 17, and Interleukin 25

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