Immunoadjuvant activity of interferon-γ-liposomes co-administered with influenza vaccines

Slooten, M. L. van; Hayon, I; Babai, Ilan; Zakay‐Rones, Zichria; Wagner, Ernst; Storm, Gert; Kedar, Eli

doi:10.1016/s1388-1981(01)00092-0

Cited by 23 publications

(13 citation statements)

References 36 publications

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“…Several studies by other authors also showed limited effect of liposomes and demonstrated that the effect could be increased if other adjuvant active compounds were used in addition to liposomes (Alving and Richards, 1990;Lipford et al, 1994;van Slooten et al, 2001;Childers et al, 2000;Baca-Estrada et al, 1999). Adjuvant activity of peptidoglycan monomer has been documented in several studies but only one report concerns its activity in liposomal formulation (Tomašic and Hršak, 1982).…”

Section: Discussionmentioning

confidence: 99%

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Effect of Liposomal Formulations and Immunostimulating Peptidoglycan Monomer (PGM) on the Immune Reaction to Ovalbumin in Mice

Habjanec

Frkanec

Halassy

et al. 2006

Journal of Liposome Research

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The adjuvant activity of liposomes and immunostimulating peptidoglycan monomer (PGM) in different formulations has been studied in mice model using ovalbumin (OVA) as an antigen. PGM is a natural compound of bacterial origin with well-defined chemical structure: GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDpm(epsilonNH2)-D-Ala-D-Ala. It is a non-toxic, non-pyrogenic, and water-soluble immunostimulator. The aim of this study was to investigate the influence of different liposomal formulations of OVA, with or without PGM, on the production of total IgG, as well as of IgG1 and IgG2a subclasses of OVA-specific antibodies (as indicators of Th2 and Th1 type of immune response, respectively). CBA mice were immunized s.c. with OVA mixed with liposomes, OVA with PGM mixed with liposomes, OVA encapsulated into liposomes and OVA with PGM encapsulated into liposomes. Control groups were OVA in saline, OVA with PGM in saline, and OVA in CFA/IFA adjuvant formulation. The entrapment efficacy of OVA was monitored by HPLC method. The adjuvant activity of the mixture of OVA and empty liposomes, the mixture of OVA, PGM, and liposomes and PGM encapsulated with OVA into liposomes on production of total anti-OVA IgG was demonstrated. The mixture of PGM and liposomes exhibited additive immunostimulating effect on the production of antigen-specific IgGs. The analysis of IgG subclasses revealed that encapsulation of OVA into liposomes favors the stimulation of IgG2a antibodies, indicating the switch toward the Th1 type of immune response. When encapsulated into liposomes or mixed with liposomes, PGM induced a switch from Th1 to Th2 type of immune response. It could be concluded that appropriate formulations of antigen, PGM, and liposomes differently affect the humoral immune response and direct the switch in the type of immune response (Th1/Th2).

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Section: Discussionmentioning

confidence: 99%

“…In several previous studies different immunomodulators have been used for enhancing the immune response to liposomal antigens (Alving and Richards, 1990;Lipford et al, 1995;van Slooten et al, 2001;Childers et al, 2000;Baca-Estrada et al, 1999). The immunomodulator of our interest is peptidoglycan monomer (PGM).…”

Section: Introductionmentioning

confidence: 99%

Effect of Liposomal Formulations and Immunostimulating Peptidoglycan Monomer (PGM) on the Immune Reaction to Ovalbumin in Mice

Habjanec

Frkanec

Halassy

et al. 2006

Journal of Liposome Research

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“…IFN-␥ targeting with colloidal systems, such as liposomes and microparticles, has led to both a reduction in toxicity and an increase in the efficacy of IFN-␥ against a great variety of intracellular parasites, such as Leishmania donovani (5) and Klebsiella pneumoniae (28). IFN-␥ loaded into liposomes was also effective as an adjuvant with antiviral vaccines (30) and antitumor vaccines (31). Another interesting approach that could increase the therapeutic efficiency of IFN-␥ may be to load it into albumin nanoparticles (IFN-␥-NPs).…”

mentioning

confidence: 99%

Gamma Interferon Loaded onto Albumin Nanoparticles: In Vitro and In Vivo Activities against Brucella abortus

Segura

Gamazo

Irache

et al. 2007

Antimicrob Agents Chemother

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The aim of this study was to evaluate the activity of gamma interferon (IFN-␥) when it was either adsorbed onto or loaded into albumin nanoparticles. Brucella abortus-infected macrophages and infected BALB/c mice were selected as the models for testing of the therapeutic potentials of these cytokine delivery systems, in view of the well-established role of IFN-␥-activated macrophages for the control of Brucella sp. infections. Whereas the encapsulation of IFN-␥ inside the matrix of nanoparticles completely abrogated its activity, adsorbed IFN-␥ increased by 0.75 log unit the bactericidal effect induced by RAW macrophages activated with free IFN-␥, along with a higher level of production of nitric oxide. In infected BALB/c-mice, IFN-␥ adsorbed onto nanoparticles was also more active than free cytokine in reducing the number of bacteria in the spleens, and the effect was mediated by an increased ratio of IFN-␥-secreting (Th1) to interleukin-4-secreting (Th2) cells. Overall, albumin nanoparticles would be suitable as carriers that target IFN-␥ to macrophages and, thus, potentiate their therapeutic activity.

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“…Gamma interferon (IFN-␥) is a key cytokine in controlling mycobacterial infection; however, little is known about its effect when it is added to subunit or DNA vaccines against M. tuberculosis. Moreover, IFN-␥ has been successfully used as an adjuvant in vaccines against other pathogens (2,16,47) and found to be safe in immunocompromised mice (23). We therefore analyzed here the effect of IFN-␥ as an adjuvant in subunit vaccines against M. tuberculosis.…”

mentioning

confidence: 99%

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

2005

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In this study, we examined the immunogenicity and protective efficacy of six immunodominant Mycobacterium tuberculosis recombinant antigens (85B, 38kDa, ESAT-6, CFP21, Mtb8.4, and 16kDa) in a multivalent vaccine preparation (6Ag). Gamma interferon (IFN-␥) and monophosphoryl lipid A-trehalose dicorynomycolate (Ribi) adjuvant systems were used separately or in combination for immunization with the recombinant antigens. Our results demonstrate that immunization of mice with Ribi emulsified antigens in the presence of IFN-␥ (Ribi؉6Ag؉IFN-␥) resulted after challenge with a virulent M. tuberculosis strain in a significant reduction in the CFU counts that was comparable to that achieved with the BCG vaccine (ϳ0.9-log protection). Antigen-specific immunoglobulin G (IgG) titers in the Ribi؉6Ag؉IFN-␥-immunized mice were lower than in mice immunized with Ribi؉6Ag and were oriented toward a Th1-type response, as confirmed by elevated IgG2a levels. In addition, splenocyte proliferation, IFN-␥ secretion, and NO production were significantly higher in splenocytes derived from Ribi؉6Ag؉IFN-␥-immunized mice, whereas IL-10 secretion was decreased. These findings confirm the induction of a strong cellular immunity in the vaccinated mice that correlates well with their enhanced resistance to M. tuberculosis. The adjuvant effect of IFN-␥ was dose dependent. A dose of 5 g of IFN-␥ per mouse per immunization gave optimal protection, whereas lower or higher amounts (0.5 or 50 g/ mouse) of IFN-␥ failed to enhance protection.

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Immunoadjuvant activity of interferon-γ-liposomes co-administered with influenza vaccines

Cited by 23 publications

References 36 publications

Effect of Liposomal Formulations and Immunostimulating Peptidoglycan Monomer (PGM) on the Immune Reaction to Ovalbumin in Mice

Effect of Liposomal Formulations and Immunostimulating Peptidoglycan Monomer (PGM) on the Immune Reaction to Ovalbumin in Mice

Gamma Interferon Loaded onto Albumin Nanoparticles: In Vitro and In Vivo Activities against Brucella abortus

Gamma Interferon and Monophosphoryl Lipid A-Trehalose Dicorynomycolate Are Efficient Adjuvants forMycobacterium tuberculosisMultivalent Acellular Vaccine

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