Immunoaffinity monolithic capillary microextraction coupled with ICP-MS for immunoassay with quantum dot labels

Immobilization of Antibodies and Enzymes on 3-Aminopropyltriethoxysilane-Functionalized Bioanalytical Platforms for Biosensors and Diagnostics

“…47 Goat antihuman IgG is immobilized on APTES−silica hybrid monolithic capillary and binds to human IgG in a sample. Subsequently, goat antihuman IgG labeled with CdSe@ZnS QDs is added to bind to the human IgG.…”

Recent advances in capillary microextraction

“…Immunoaffinity monolithic CME was also reported by Hu et al [33]. They developed a new method for immunoassay by coupling immunoaffinity monolithic CME to micro-concentric nebulizer (MCN)-ICP-MS with quantum-dot (QD) labels.…”

“…Sandwich-type immunoassay based upon capillary microextraction: a) antibody immobilized onto hybrid monolithic capillary; b) the capillary is blocked with BSA; c) human IgG antigen is incubated; d) the secondary antibody with CdSe-nanoparticle labels is bound; and, e) the label is dissolved by eluent (PBS + formic acid, pH:2) and released Cd and Se are detected by MCN-ICP-MS[33].…”

A sensitive atomic absorption spectrometric metalloimmunoassay with copper nanoparticles labeling

“…Zhang et al [12] and Tanner et al [13] reported the pioneer work about various kinds of ICPMS-based metalloimmunoassays by Au nanoparticles [14][15][16][17][18][19][20][21][22] labeling. CdSe quantum dots [23], PbS quantum dots [24] and silver nanoparticles [25] were employed by Hu et al for disease biomarker quantification by ICPMS. HgS nanoparticles [26] were synthesized by Lv et al for high sensitivity in AFS.…”