Immunoanalytical techniques for analyzing pesticides in the environment

Suri, C. Raman; Boro, Robin Chandra; Nangia, Yogesh; Gandhi, Sonu; Sharma, Priyanka; Wangoo, Nishima; Rajesh, Kumar; Shekhawat, Gajendra S.

doi:10.1016/j.trac.2008.09.017

Cited by 134 publications

(29 citation statements)

References 42 publications

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“…Enzyme labeling is a time consuming and complicated procedure. Suri et al (2009) provide an elaborate discussion of immunoanalytical techniques for pesticide analysis. Rahman et al (2007) have fabricated a label-free impedimetric immunosensor for the direct detection of BPA.…”

Section: New Generation Immunosensorsmentioning

confidence: 99%

Treatment of Micropollutants in Water and Wastewater

Virkutytė

Varma

2010

wio

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The Integrated Environmental Technology Series addresses key themes and issues in the field of environmental technology from a multidisciplinary and integrated perspective.An integrated approach is potentially the most viable solution to the major pollution issues that face the globe in the 21st century.World experts are brought together to contribute to each volume, presenting a comprehensive blend of fundamental principles and applied technologies for each topic. Current practices and the state-of-the-art are reviewed, new developments in analytics, science and biotechnology are presented and, crucially, the theme of each volume is presented in relation to adjacent scientific, social and economic fields to provide solutions from a truly integrated perspective.The Integrated Environmental Technology Series will form an invaluable and definitive resource in this rapidly evolving discipline.

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Section: New Generation Immunosensorsmentioning

confidence: 99%

Treatment of Micropollutants in Water and Wastewater

Virkutytė

Varma

2010

wio

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“…Combinatory chromatographic methods have been used to determine the presence of atrazine in both serum and urine [17,18]. Antibody-based assays are also used, however the antibody used cannot differentiate between atrazine and closely-related molecules [19,20]. There also has been a DNA binding element previously selected for atrazine which had a dissociation constant of 890 nM [21].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine

Williams

Crihfield

Gattu

et al. 2014

IJMS

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Widespread use of the chlorotriazine herbicide, atrazine, has led to serious environmental and human health consequences. Current methods of detecting atrazine contamination are neither rapid nor cost-effective. In this work, atrazine-specific single-stranded DNA (ssDNA) molecular recognition elements (MRE) were isolated. We utilized a stringent Systematic Evolution of Ligands by Exponential Enrichment (SELEX) methodology that placed the greatest emphasis on what the MRE should not bind to. After twelve rounds of SELEX, an atrazine-specific MRE with high affinity was obtained. The equilibrium dissociation constant (Kd) of the ssDNA sequence is 0.62 ± 0.21 nM. It also has significant selectivity for atrazine over atrazine metabolites and other pesticides found in environmentally similar locations and concentrations. Furthermore, we have detected environmentally relevant atrazine concentrations in river water using this MRE. The strong affinity and selectivity of the selected atrazine-specific ssDNA validated the stringent SELEX methodology and identified a MRE that will be useful for rapid atrazine detection in environmental samples.

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“…There are at least four classification of immunoassay at present: optical, electrochemical, piezoelectric (PZ) and micromechanical (Raman Suri et al, 2009), all of which depend on Abs and sensitive components. Two kinds of immunosensor have been developed for determination of aflatoxin (i.e.…”

Section: Biosensormentioning

confidence: 99%

Aflatoxin Measurement and Analysis

Hussain¹

2011

Aflatoxins - Detection, Measurement and Control

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Immunoanalytical techniques for analyzing pesticides in the environment

Cited by 134 publications

References 42 publications

Treatment of Micropollutants in Water and Wastewater

Treatment of Micropollutants in Water and Wastewater

In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Atrazine

Aflatoxin Measurement and Analysis

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