“…Haque et al (1997) highlighted the risks of relying one's analysis only on microscopy after that of all the children in Bangladesh diagnosed by microscopy with amebiasis, only 40% were positive by antigen detection and culture isoenzyme analysis, that, even more, detected new infections missed by microscopy. This is in line with another study in rural communities in Nigeria where authors reported the detection of 6 additional cases with immunoassay compared to the diagnosis by microscopy, with a distinction between the pathogenic and non pathogenic species (Babatunde et al, 2016).…”