Immunoassay methods based on fluorescence polarization

Gutiérrez, M.C.; Gómez‐Hens, A.; Pérez‐Bendito, Dolores

doi:10.1016/0039-9140(89)80050-5

Cited by 37 publications

(20 citation statements)

References 103 publications

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“…The principle of FPIA and its application in drug residues field could be found in several excellent reviews [6,7]. In this work, we developed an FPIA for the rapid determination of SAL.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

et al. 2010

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A fluorescence polarization immunoassay (FPIA) for the determination of salinomycin (SAL) was developed by using anti-SAL monoclonal antibodies (mAb). Fluorescein labeled SAL (tracer) was synthesized by the N-hydroxysuccinimide active ester method and purified using thin layer chromatography (TLC). The developed FPIA for SAL had a dynamic range from 0.60 to 2193 ng/mL with an IC 50 value of 33.2 ng/mL and a detection limit (LOD) of 0.08 ng/mL. No significant cross-reactivities were observed with other drugs but 67.6% with narasin. salinomycin, tracer, fluorescence polarization immunoassay, monoclonal antibody

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“…The principle of FPIA and its application in drug residues field could be found in several excellent reviews [6,7]. In this work, we developed an FPIA for the rapid determination of SAL.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

et al. 2010

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“…FPIA is a competitive homogeneous immunoassay method based on differences in polarisation degree of the fluorescein‐labelled analytes in the free and antibody‐bound fractions in solution. The principles of FPIA have been reviewed (Gutierrez et al. , 1989; Eremin & Smith, 2003; Jolley & Nasir, 2003).…”

Section: Introductionmentioning

confidence: 99%

Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

Wang

Zhang

Murtazina

et al. 2007

Int J of Food Sci Tech

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A fluorescence polarisation immunoassay (FPIA) using a specific polyclonal antiserum for the detection of maduramicin (MD) was developed and optimised. The polyclonal antiserum was produced against MD linked to bovine serum albumin. Fluorescein-labelled MD (tracer) was synthesised by N-hydroxysuccinimide active ester method and purified using thin layer chromatography. The developed FPIA for MD had a dynamic range from 0.01 to 5.6 lg mL )1 with an IC 50 value of 0.16 lg mL )1 and a limit of detection of 0.002 lg mL )1 . Recoveries from chicken muscle, fat and egg samples spiked at 0.25, 5 and 10 lg g )1 levels were 82-130%. The FPIA results from analysis of incurred residues in chicken muscle samples showed that the simple procedure is viable.

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“…This technique was considered in detail in a number of reviews [27][28][29]. FPIA is based on the competitive interaction of an analyte (antigen) and an antigen with a fluorescent label (tracer) for a limited number of specific antibody binding sites and on the detection of the fluorescence polarization of the reaction mixture.…”

Section: Acetochlormentioning

confidence: 99%

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

et al. 2005

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Acetochlor[2-chloro-N -(ethoxymethyl)-N -(2-ethyl-6-methylphenyl)acetamide] belongs to chloroacetanilide herbicides, which have been widely introduced into the world agricultural practice [1][2][3]. Chloroacetanilide herbicides are relatively unstable in the environment and undergo degradation within six months; this is a valuable property of these herbicides. However, they can be detected in reservoirs, fish, and products of plant origin in amounts higher than maximum permissible levels as a result of their agricultural use. Acetochlor is a comparatively new pesticide; it was first registered in the United States in 1994. It has now replaced related compounds because of its better biodegradability and a relatively small carcinogenic effect. However, as well as the majority of pesticides, acetochlor is genotoxic, as was exemplified in plants, rats, and human lymphocytes [1]. Therefore, acetochlor residues should be monitored in natural samples such as water and soil.Chromatographic techniques, such as gas chromatography with atomic emission [4] or mass-spectrometric detection [5, 6], high-performance liquid chromatography with various detection techniques [7,8], and capillary electrophoresis [9] are commonly used for determining herbicides, in particular, acetochlor. These techniques are highly sensitive and accurate; however, they are also expensive and time-consuming. At the same time, it is necessary to perform simple and rapid screening in a great number of samples in order to detect herbicide-contaminated areas.Immunochemical techniques based on the specific binding of an analyte to corresponding specific antibodies are well suitable for this purpose. Enzyme immunoassay (EIA) has received the widest acceptance; the theory and practice of EIA was described in detail [10]. The use of EIA for the determination of pesticides has been reported in many reviews [11][12][13][14][15][16].EIA techniques are highly sensitive and simple; they can be performed under field conditions at the sampling site [17]. Since the 1990s, EIA has been used with increasing frequency for the screening of pesticides in natural and food samples. The current status, applicability, and prospects for the widespread use of immunochemical techniques for the determination of pesticides in the environment were considered in a review [18]. Recent data on the development and use of EIA for pesticides in Russia were published in [19][20][21].Earlier, we developed immunochemical methods for the determination of acetochlor by ELISA [22] and with the use of an immunosensor [23] or a piezosensor [24]. However, these are multistep procedures, and the time of analysis is 2 to 3 h. Moreover, ELISA includes a number of steps of pipetting and washing with the use of several specific reagents; it requires qualified personnel. Therefore, the recent trend has been toward the development of nonseparation (homogeneous) immunoassay techniques, which significantly improve socalled high-throughput screening (HTS) [25]. Although an optimum immunoassay procedure for...

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Immunoassay methods based on fluorescence polarization

Cited by 37 publications

References 103 publications

Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

Fluorescence polarization immunoassay for salinomycin based on monoclonal antibodies

Determination of the veterinary drug maduramicin in food by fluorescence polarisation immunoassay

Determination of the herbicide acetochlor by fluorescence polarization immunoassay

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