Immunoassay methods: Development and implementation programme at the USEPA

Lesnik, Barry

doi:10.1080/09540109409354836

Cited by 15 publications

(4 citation statements)

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“…Of twenty-one OP-free water samples, one false positive result was found. The false positive rate was about 4.8%, which is lower than the tolerable false positive rate for a screening method (10% to 25%) set by the U.S. Environmental Protection Agency’s Office of Solid Waste …”

Section: Resultsmentioning

confidence: 67%

“…The false positive rate was about 4.8%, which is lower than the tolerable false positive rate for a screening method (10% to 25%) set by the U.S. Environmental Protection Agency's Office of Solid Waste. 30 The results indicated that the proposed SPE−dcCL-EIA is ideally suited as a monitoring method for OP residues prior to chromatographic analysis. It is able to pick out positive samples from a large number of water samples.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 90%

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Development of a Solid-Phase Extraction Coupling Chemiluminescent Enzyme Immunoassay for Determination of Organophosphorus Pesticides in Environmental Water Samples

Sun

Yang

et al. 2012

J. Agric. Food Chem.

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Solid-phase extraction (SPE) and direct competitive chemiluminescence enzyme immunoassay (dcCL-EIA) were combined for the detection of organophosphorus pesticides (OPs) in environmental water samples. dcCL-EIA based on horseradish peroxidase labeled with a broad-specificity monoclonal antibody against OPs was developed, and the effects of several physicochemical parameters on dcCL-EIA performance were studied. SPE was used for the pretreatment of water samples to remove interfering substances and to concentrate the OP analytes. The coupling of SPE and dcCL-EIA can detect seven OPs (parathion, coumaphos, phoxim, quinalphos, triazophos, dichlofenthion, and azinphos-ethyl) with the limit of quantitation below 0.1 ng/mL. The recoveries of OPs from spiked water samples ranged from 62.5% to 131.7% by SPE-dcCL-EIA and 69.5% to 112.3% by SPE-HPLC-MS/MS. The screening of OP residues in real-world environmental water samples by the developed SPE-dcCL-EIA and their confirmatory analysis using SPE-HPLC-MS/MS demonstrated that the assay is ideally suited as a monitoring method for OP residues prior to chromatographic analysis.

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Section: Resultsmentioning

confidence: 67%

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 90%

Development of a Solid-Phase Extraction Coupling Chemiluminescent Enzyme Immunoassay for Determination of Organophosphorus Pesticides in Environmental Water Samples

Sun

Yang

et al. 2012

J. Agric. Food Chem.

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“…The Data Quality Objectives (DQO) process (1-3) is a sevenstep systematic planning approach based on the scientific method recommended by the U.S. Environmental Protection Agency (EPA) and other agencies for project planning to characterize and clean up waste sites. The seven steps of the DQO process are as follows: (1) state the problem; (2) identify the decision; (3) identify the inputs to the decision; (4) define the boundaries of the study; (5) develop a decision rule; (6) specify limits on decision errors; and (7) optimize the design.…”

Section: Introductionmentioning

confidence: 99%

Meeting Data Quality Objectives with Interval Information

Bayne

Dindal

Jenkins

et al. 2001

Environ. Sci. Technol.

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Immunoassay test kits are promising technologies for measuring analytes under field conditions. Frequently, these field-test kits report the analyte concentrations as falling in an interval between minimum and maximum values. Many project managers use field-test kits only for screening purposes when characterizing waste sites because the results are presented as semiquantitative intervals. However, field-test kits that report results as intervals can also be used to make project-related decisions in compliance with false-rejection and false-acceptance decision error rates established during a quantitative data quality objective process. Sampling and analysis plans can be developed that rely on field-test kits to meet certain data needs of site remediation projects.

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“…Currently, OSW has issued more than fifteen immunoassay methods using several different techniques, which can be used for analyses performed under the Resource Conservation and Recovery Act (RCRA). 2…”

Section: Introductionmentioning

confidence: 99%

Immunoassay Techniques in Environmental Analyses

Lesnik

2000

Encyclopedia of Analytical Chemistry

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Introduction 1 2 Background and History 2 2.1 Overview of United States Environmental Protection Agency Regulatory Programs 2 2.2 Developmental History of Immunoassay Methods in United States Environmental Protection Agency Programs 3 3 Types of Immunoassay Technologies Employed in Environmental Programs 4 3.1 Enzyme-linked Immunosorbent Assay 4 3.2 Reporter Gene on a Human Cell Line 5 3.3 Immunosensors 6 3.4 Affinity Chromatography 6 3.5 Dissociation-enhancement Lanthanide Fluoroimmunoassay 6 4 Method Development 6 4.1 General Guidelines for Development of Screening Methods 6 4.2 Validation Criteria for Immunoassay Methods 7 5 Status of the United States Environmental Protection Agency Immunoassay Methods Development Program 8 5.1 Current Validated Methods 8 5.2 Methods under Development 9 5.3 List of Validated Immunoassay Test Products 11 6 Environmental Applications 11 6.1 Advantages and Disadvantages of Immunoassay Methods 11 6.2 Implementation Issues 12 6.3 Examples of Performance Based Measurement Systems 13 6.4 Applications as Screening Methods 13 6.5 Applications as Quantitative Methods 14 7 Future Directions 15 Acknowledgments 15 Abbreviations and Acronyms 15 Related Articles 16 References 16

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Immunoassay methods: Development and implementation programme at the USEPA

Cited by 15 publications

References 0 publications

Development of a Solid-Phase Extraction Coupling Chemiluminescent Enzyme Immunoassay for Determination of Organophosphorus Pesticides in Environmental Water Samples

Development of a Solid-Phase Extraction Coupling Chemiluminescent Enzyme Immunoassay for Determination of Organophosphorus Pesticides in Environmental Water Samples

Meeting Data Quality Objectives with Interval Information

Immunoassay Techniques in Environmental Analyses

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