Immunoassay of cardiac biomarkers using a photodiode array biochip

Han, Kwi Nam; Le, Thanh‐Hai; Pham, Xuan‐Hung; Huynh-Nguyen, Buu-Chau; Kim, Ji Hye; Ko, Euna; Kwon, Ho Taik; Seong, Gi Hun

doi:10.1016/j.snb.2014.10.014

Cited by 5 publications

(6 citation statements)

References 38 publications

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“…Han et al have made use of this relationship in the development of a photodiode array biochip by using a NHS-LC-Biotin kit as a means to create biotinylated Abs. The formation of this detection probe was dependent on the binding of the biotinylated Abs with streptavidin conjugated AuNPs [74]. The biosensor utilised both monoclonal and polyclonal Abs in the sandwich-based immunoassay, although more significantly, the biotinylated strategy employed produces randomly orientated Abs.…”

Section: Immobilisation Strategiesmentioning

confidence: 99%

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Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

2018

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Cardiac biomarkers are frequently measured to provide guidance on the well-being of a patient in relation to cardiac health with many assays having been developed and widely utilised in clinical assessment. Effectively treating and managing cardiovascular disease (CVD) relies on swiftly responding to signs of cardiac symptoms, thus providing a basis for enhanced patient management and an overall better health outcome. Ultra-sensitive cardiac biomarker detection techniques play a pivotal role in improving the diagnostic capacity of an assay and thus enabling a better-informed decision. However, currently, the typical approach taken within healthcare depends on centralised laboratories performing analysis of cardiac biomarkers, thus restricting the roll-out of rapid diagnostics. Point-of-care testing (POCT) involves conducting the diagnostic test in the presence of the patient, with a short turnaround time, requiring small sample volumes without compromising the sensitivity of the assay. This technology is ideal for combatting CVD, thus the formulation of ultra-sensitive assays and the design of biosensors will be critically evaluated, focusing on the feasibility of these techniques for point-of-care (POC) integration. Moreover, there are several key factors, which in combination, contribute to the development of ultra-sensitive techniques, namely the incorporation of nanomaterials for sensitivity enhancement and manipulation of labelling methods. This review will explore the latest developments in cardiac biomarker detection, primarily focusing on the detection of cardiac troponin I (cTnI). Highly sensitive detection of cTnI is of paramount importance regarding the rapid rule-in/rule-out of acute myocardial infarction (AMI). Thus the challenges encountered during cTnI measurements are outlined in detail to assist in demonstrating the drawbacks of current commercial assays and the obstructions to standardisation. Furthermore, the added benefits of introducing multi-biomarker panels are reviewed, several key biomarkers are evaluated and the analytical benefits provided by multimarkers-based methods are highlighted.

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Section: Immobilisation Strategiesmentioning

confidence: 99%

“…Figure 3 and Figure 4: [37,38,48,49,50,57,58,61,65,66,68,69,70,71,74,84,85,86,87,88,89,93,94,95,96,97,104,105,111,112,115,116,117,120,121,122,123,125,126,127,128,130,132,133,134,136,138,144,145,148,150,151,159,161,174,175,176,184,185,186,187,188,189,190,…”

Section: Reprinting Figuresmentioning

confidence: 99%

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

2018

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“…Thanks to the development of the silicon solid-state photoelectric technology, photosensitive devices such as photodiodes (PDs) [6,7], PD array (PDA) chips [8], and silicon photomultipliers (SiPMs) [9,10] have been produced with micro-packaging and high sensitivity, which fit the requirement for the miniaturized ECL instrument [6][7][8][9][10]. The SiPM, also called multi-pixel photon counter (MPPC), is a new type of photon-counting device made up of multiple avalanche PD (APD) pixels operated in Geiger mode [11].…”

Section: Introductionmentioning

confidence: 99%

Development of Miniaturized Electrochemiluminescence Instrument using Multi‐pixel Photon Counter as the Optical Detector

Dai

Zhao

et al. 2020

Electroanalysis

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We developed a miniaturized electrochemiluminescence (ECL) instrument coupled with a light‐emitting diode‐based bipolar electrochemical sensor (LED‐BPES). This instrument composes of a microcontroller circuit, a power supply circuit, a potentiostat, an optical detecting circuit, and a communication circuit. The multi‐pixel photon counter (MPPC), which is low‐cost, small‐size, and wide‐range in optical measurements, is chosen as the optical detector. The LED‐BPES composes of a disposable screen‐printed carbon electrode (SPCE) and a surface‐mount red LED. Depended on the closed bipolar electrode (C‐BPE) structure, the LED‐BPES not only avoids the employment of unstable and complex ECL reactions but also offers a cost‐effective alternative for the over‐priced ECL reagents by using a mini‐size commercial LED as the luminescent producer. The combination of MPPC and LED‐BPES helps to set up the simplified and downsized instrument system with low price and high efficiency. The presented instrument coupled with LED‐BPES works excellent in electroactive molecules detection and has great potential in the application of heavy metal ions detection.

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“…Immunogold labeling technique has been widely used to amplify analytical signal for bio-recognition applications similar to horseradish peroxidase (HRP) in ELISA due to the high electron density of gold nanoparticles (AuNps) as well as the further signal amplification through immunogold silver staining [17][18][19][20][21][22]. Guo et al showed the good prospect of immunogold assay in real clinical applications by reducing an assay time to 40 min which is much faster than the traditional ELISA that takes usually at least 3 h for a turnaround test [23].…”

Section: Introductionmentioning

confidence: 99%

“…However, the detection limit of the assay for cTnI sensing in serum samples was still limited to be 1 ng/mL similar to those detected by ELISA, requiring a more sensitive immunoassay procedure or sensor platform. Recently, many studies were reported to improve the sensitivity of the immunogold-based assay for the detection of cardiac markers by using different sensor platforms such as a surface acoustic wave immunosensor [24], electrochemical immunosensors [16], photodiode array [20], and colorimetric assay [25]. The previously reported immunogold assays were achieved by a direct assay where the immunoreaction between the target antigen and the mab-cTnI coated on the well undergoes in advance.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive detection of cardiac troponin I in human serum using gold nanoparticle-based enhanced sandwich immunoassay

Kim

Cho

Kim

et al. 2015

Sensors and Actuators B: Chemical

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Immunoassay of cardiac biomarkers using a photodiode array biochip

Cited by 5 publications

References 38 publications

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

Point-of-Care Compatibility of Ultra-Sensitive Detection Techniques for the Cardiac Biomarker Troponin I—Challenges and Potential Value

Development of Miniaturized Electrochemiluminescence Instrument using Multi‐pixel Photon Counter as the Optical Detector

Highly sensitive detection of cardiac troponin I in human serum using gold nanoparticle-based enhanced sandwich immunoassay

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