Immunobiology and Immunodiagnosis of Echinostomiasis

Graczyk, Thaddeus K.

doi:10.1007/978-94-015-9606-0_12

Cited by 3 publications

(1 citation statement)

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“…Although several studies on the humoral response generated against E. caproni in the definitive host have been performed in the past (Agger et al 1993;Brunet et al 2000;Graczyk, 2000, Sotillo et al 2007, the effect of glycosylation on the antibody response against E. caproni has been poorly studied. Simonsen and Andersen (1986) described an intense humoral response in experimentally infected mice against glycosylated antigens, though the extent of the influence in this response of glycans was not determined.…”

Section: Introductionmentioning

confidence: 99%

The effect of glycosylation of antigens on the antibody responses againstEchinostoma caproni(Trematoda: Echinostomatidae)

et al. 2014

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In the present study, we analyse the effect of glycosylation in Echinostoma caproni (Trematoda: Echinostomatidae) antigens in antibody responses against the parasite in experimentally infected mice. It has been previously demonstrated that the mouse is a host of high compatibility with E. caproni and develops elevated responses of IgG, IgG1, IgG3 and IgM as a consequence of the infection, though the role of glycans in these responses remains unknown. To this purpose, the responses generated in mice against non-treated excretory/secretory antigens of E. caproni were compared with those observed after N-deglycosylation, O-deglycosylation and double deglycosylation of the antigens by indirect ELISA and western blot. Our results suggest that E. caproni-expressed glycans play a major role in the modulation of the immune responses. The results obtained indicate that IgG subclass responses generated in mice against E. caproni are essentially due to glycoproteins and may affect the Th1/Th2 biasing. The reactivity significantly decreased after any of the deglycosylation treatments and the N-glycans appears to be of greater importance than O-glycans. Interestingly, the IgM response increased after N-deglycosylation suggesting that carbohydrates may mask peptide antigens.

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