The effect of glycosylation of antigens on the antibody responses againstEchinostoma caproni(Trematoda: Echinostomatidae)

Abstract

In the present study, we analyse the effect of glycosylation in Echinostoma caproni (Trematoda: Echinostomatidae) antigens in antibody responses against the parasite in experimentally infected mice. It has been previously demonstrated that the mouse is a host of high compatibility with E. caproni and develops elevated responses of IgG, IgG1, IgG3 and IgM as a consequence of the infection, though the role of glycans in these responses remains unknown. To this purpose, the responses generated in mice against non-treated excretory/secretory antigens of E. caproni were compared with those observed after N-deglycosylation, O-deglycosylation and double deglycosylation of the antigens by indirect ELISA and western blot. Our results suggest that E. caproni-expressed glycans play a major role in the modulation of the immune responses. The results obtained indicate that IgG subclass responses generated in mice against E. caproni are essentially due to glycoproteins and may affect the Th1/Th2 biasing. The reactivity significantly decreased after any of the deglycosylation treatments and the N-glycans appears to be of greater importance than O-glycans. Interestingly, the IgM response increased after N-deglycosylation suggesting that carbohydrates may mask peptide antigens.

N-Glycosylation of mollusk hemocyanins contributes to their structural stability and immunomodulatory properties in mammals

“…These results suggest that hemocyanin carbohydrates participate in the specific humoral response, which is mediated by B lymphocytes that recognize various antigens, including oligosaccharides (65, 66). Indeed, anti-glycan antibodies have been previously reported; for instance, infections by some glycosylated parasites, such as Schistosoma mansoni , Toxocara canis , and Hemonchus contortus , among others, efficiently induce anti-glycan IgM and IgG (66, 67). Actually, specific serum against CCH, FLH, and KLH shows a decreased reactivity against hemocyanins in the presence of soluble mannose and galactose.…”

Tegument Glycoproteins and Cathepsins of Newly Excysted Juvenile Fasciola hepatica Carry Mannosidic and Paucimannosidic N-glycans

“…Glycomic studies of other trematodes, such as Schistosoma mansoni [ 17 ] and Opisthorchis viverrini [ 18 ] and most recently Echinostoma caproni [ 19 , 20 ] have led to a deeper understanding of the structural and functional aspects of glycans in this class of helminths. The immunomodulatory properties of F .…”

Schistosome-Derived Molecules as Modulating Actors of the Immune System and Promising Candidates to Treat Autoimmune and Inflammatory Diseases

“…Biotechnological advances are offering new systems to generate recombinant proteins in eukaryotic cells, which allow higher yields of purified proteins with their posttranslation modifications such as glycosylations and acylations [93–96]. These biotechnological tools can be essential to obtain isolated molecules in their native and original form, a characteristic that is largely vital for glycoproteins and lipoproteins activity and antigenicity as previously demonstrated for helminth molecules in some publications [58, 97]. In recent years, many proteomic studies revealed the composition of peptides of soluble egg antigens (SEA), adult soluble worm antigen (SWA) preparation, cercariae, schistosomula tegument, and adult worm tegument [98–106] which, altogether, represent a vast repertoire of potential proteins to be evaluated in combinations or isolated in order to find key immunomodulatory proteins within these different and complex molecular mixtures from schistosomes.…”