Immunobiology of chicken germinal center: I. Changes in surface Ig class expression in the chicken splenic germinal center after antigenic stimulation

Yasuda, Masahiro; Kajiwara, Eiji; Ekino, Shigeo; Taura, Yasuho; Hirota, Yoshikazu; Horiuchi, Hiroyuki; Matsuda, Haruo; Furusawa, Shuichi

doi:10.1016/s0145-305x(02)00066-6

Cited by 28 publications

(27 citation statements)

References 24 publications

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“…We also reported that immunoglobulin class switching takes place within GCs (Yasuda et al 2003). In the present study, we detected apoptosis during the chicken splenic GC reaction in response to antigenic stimulation using TUNEL and small amount DNA ladder assay.…”

Section: Introductionmentioning

confidence: 69%

“…However, there is no information on the mechanism for the disappearance of GC structures. Studies have demonstrated that the number of chicken splenic GCs increase up to 7 days after immunization, and then decrease by 21 days (Liu et al 1991b;Yasuda et al 2003). GCs are thought to be associated with the development of B-cell memory and to be the site of somatic hypermutation, selection and differentiation during the immune response in mammals (Liu et al 1989;Leanderson et al 1992;Ford et al 1994;Grandien et al 1994).…”

Section: Introductionmentioning

confidence: 99%

“…We previously established a methodology for isolating a single GC from spleen and observed a considerable difference in the cell populations of each GC (Yasuda et al 2003). We also reported that immunoglobulin class switching takes place within GCs (Yasuda et al 2003).…”

Section: Introductionmentioning

confidence: 99%

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Immunobiology of chicken germinal center: II. Accumulation of apoptotic cells within the germinal center

Yasuda

Horiuch

Matsuda

et al. 2003

Cell and Tissue Research

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The germinal center (GC) develops after antigen stimulation and is thought to occur at the site of various immune responses. We observed apoptotic cells within the GC using in situ end labeling (TUNEL), small amount DNA ladder assay, and RT-PCR analysis of Bcl-2 mRNA expression. Apoptosis was detected within GCs at all phases of the GC reaction by both TUNEL and DNA ladder assays. The number of TUNEL(+) nuclei within the GC did not increase over the course of the GC reaction. However, the density of DNA in the ladder assay was higher in later-phase GCs. Bcl-2 mRNA expression was detected within GCs during the early phases of the GC reaction. These results indicate that accumulation of apoptotic cells and rescue from apoptosis occur within chicken GCs. In the present paper, the reasons for the accumulation of apoptotic cells will be discussed.

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Section: Introductionmentioning

confidence: 69%

Section: Introductionmentioning

confidence: 99%

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Immunobiology of chicken germinal center: II. Accumulation of apoptotic cells within the germinal center

Yasuda

Horiuch

Matsuda

et al. 2003

Cell and Tissue Research

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“…Also of note at this stage is that IgG and IgA mRNA can both be detected in the jejunal PP follicles, but not in the ileal PP follicle [120]. Similarly, in the chicken, IgG + cells are found in the bursal follicle, as well as in germinal centers, but IgG mRNA expression is only detected in germinal centers [117,119,120]. This may be because CD4 + cells do not enter the medulla of the bursal follicle.…”

Section: Postnatal Changes In Ileal and Jejunal Pp Prior To Adolescencementioning

confidence: 87%

The sheep and cattle Peyer’s patch as a site of B-cell development

et al. 2006

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-In sheep and cattle, the ileal Peyer's patch (PP), which extends one-two meters along the terminal small intestine, is a primary lymphoid organ of B-cell development. B-cell diversity in the ileal PP is thought to develop by combinatorial mechanisms, gene conversion and/or point mutation. These species also have jejunal PP that function more like secondary lymphoid tissues concerned with mucosal immune reactions. These two types of PP differ significantly in their histology, ontogeny and the extent of lymphocyte traffic. The prenatal development of follicles in the PP begins first in the jejunum during the middle of gestation and then in the ileum during late gestation. B-cells proliferate rapidly in the ileal PP follicle; up to five percent of these cells survive while the majority dies by apoptosis, perhaps driven by the influence of environmental antigen and/ or self-antigen. The surviving cells migrate from the ileal PP and populate the peripheral B-cell compartment. By adolescence, the ileal PP has involuted but the function of jejunal PP, compatible with a role as secondary lymphoid organ, continues throughout life. In this review, we focus on the development of PP as a site of B-cell repertoire generation, positive and negative B-cell selection, and the differences between ileal PP and jejunal PP.

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“…These events occur in germinal centers (GCs) of secondary lymphoid tissues after exposure to environmental antigen. GCs are found only in mammals and birds (Yasuda et al, 2003;Vigliano et al, 2006;Du Pasquier et al, 2000). Although lacking GCs, there is SHM and CSR in Xenopus (Marr et al, 2007) although it may be less efficient.…”

Section: Maturation Of the Antibody Repertoire Involves Class Switch mentioning

confidence: 99%

Immunoglobulin Polygeny: An Evolutionary Perspective

Butler¹,

Sun²,

Wertz³

2011

Gene Duplication

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Immunobiology of chicken germinal center: I. Changes in surface Ig class expression in the chicken splenic germinal center after antigenic stimulation

Cited by 28 publications

References 24 publications

Immunobiology of chicken germinal center: II. Accumulation of apoptotic cells within the germinal center

Immunobiology of chicken germinal center: II. Accumulation of apoptotic cells within the germinal center

The sheep and cattle Peyer’s patch as a site of B-cell development

Immunoglobulin Polygeny: An Evolutionary Perspective

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