Immunobiology of grass pollen allergens

Mohapatra, Shyam S.; Lockey, Richard F.; Shirley, Shawna A.

doi:10.1007/s11882-005-0011-2

Cited by 39 publications

(42 citation statements)

References 36 publications

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“…In contrast, the GI extract, which showed less than 50% inhibition for Lm immunodominant components, presents in its composition grass pollen tribes (Triticeae and Aveneae) phylogenetically different from L. multiflorum (Poeae). Altogether, these results are consistent with inhibition radioimmunoassay and ELISA studies, using crude extracts of grass pollen of the family Poaceae that showed strong cross-reactivity between their members (1,10,36). Further inhibition experiments should be conducted with purified or recombinant major allergens to confirm the grouping of the individual allergens.…”

Section: Discussionsupporting

confidence: 83%

“…Pollen allergens from groups 1 and 5 are highly conserved in most clinically relevant grasses (33)(34)(35)(36) and proved to be important in this study. However, the 28-30-and 32-35-kDa components likely related to allergens from groups 5 and 1, respectively, showed more than 50% inhibition with the Lp and GII extracts, and less than 50% inhibition when the GI extract was used.…”

Section: Discussionmentioning

confidence: 70%

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IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis

Bernardes

Moreira

Sopelete

et al. 2010

Braz J Med Biol Res

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90% recognition. Lm, Lp and GII extracts significantly inhibited IgE binding to the most immunodominant Lm components, particularly the 55 kDa band. The 26 kDa and 90-114 kDa bands presented the lowest amount of heterologous inhibition. We demonstrated that Lm extract contains both Lm-specific and cross-reactive IgE-binding components and therefore it is suitable for measuring quantitative IgE levels for diagnostic and therapeutic purposes in patients with pollinosis sensitized to Lm grass pollen rather than other phylogenetically related grass pollen extracts.]]>

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 70%

IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis

Bernardes

Moreira

Sopelete

et al. 2010

Braz J Med Biol Res

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“…The few published studies of clinical thresholds for Poaceae pollen have been conducted in Europe, where grass species with the C3 photosynthetic pathway dominate. Investigations of protein allergens from tropical grasses, or indeed other tropical plant families, are an acknowledged gap in the available literature (Mohapatra et al, 2005;Radauer and Breiteneder, 2006). The majority of allergenic grass species characterized thus far belong to the Pooideae sub-family which has the C3 photosynthetic pathway, yet on a global scale, about one-half of all known grass species belong to a tropical functional group with the C4 photosynthetic pathway (Gaut, 2002).…”

Section: Discussionmentioning

confidence: 99%

Pollen Loads and Allergic Rhinitis in Darwin, Australia: A Potential Health Outcome of the Grass-Fire Cycle

2009

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Although the prevalence of asthma and allergic rhinitis has been increasing in tropical regions, little is known about the allergenicity of pollens from tropical plant families or the importance of ongoing environmental changes. We investigated associations between daily average pollen counts of several tropical plant families and sales of medications for the treatment of allergic rhinitis in Darwin, Australia-a tropical setting in which grass abundance has increased due to increased fire frequencies and the introduction of African pasture grasses. Daily pollen counts with detailed identification of plant species were undertaken in conjunction with a weekly survey of flowering plant species from April 2004 to November 2005. Five pharmacies provided daily sales data of selected medications commonly used to treat allergic rhinitis. We used generalized linear modeling to examine outcomes.All analyses accounted for the potential confounding effects of time trends, holidays, respiratory viral illnesses, meteorological conditions, and air pollution. The peak total pollen count was 94 grains/m 3 . Despite the low levels of Poaceae (grass) pollen (maximum daily count, 24 grains/m 3 ), there was a clear association with daily sales of anti-allergic medications greatest at a lag of 1 day. Sales increased by 5% with an interquartile range rise (3 grain/ m 3 ) in Poaceae pollen (5.07%, 95%CI 1.04%, 9.25%). No associations were observed with pollen from other plant families. Although further testing is required, we suggest that an overlooked aspect of the ''grass-fire cycle'' that is degrading many tropical landscapes, could be an increase in the prevalence of allergic rhinitis.

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“…Pollen grains of ragweed of Ambrosia species (6) and grasses of the Poaceae family, such as timothy (Phleum pratense), rye (Lolium spp. ), Kentucky blue grass (Poa pratensis), orchard grass (Dactylis glomerata), Bermuda grass (Cynodon dactylon), and others (7), are also among the most clinically relevant sources of allergens.…”

mentioning

confidence: 99%

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

Kamijo¹,

Takai²,

Kuhara³

et al. 2009

The Journal of Immunology

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Pollen is considered a source of not only allergens but also immunomodulatory substances, which could play crucial roles in sensitization and/or the exacerbation of allergies. We investigated how allergenic pollens from different plant species (Japanese cedar and Japanese cypress, which belong to the Cupressaceae family, and birch, ragweed, and grass) modulate murine bone marrow-derived dendritic cell (DC) responses and examined the effect of Cupressaceae pollen in vivo using mice. DCs were stimulated with pollen extracts or grains in the presence or absence of LPS. Cell maturation and cytokine production in DCs were analyzed by flow cytometry, ELISA, and/or quantitative PCR. Pollen extracts suppressed LPS-induced IL-12 production and the effect was greatest for birch and grass. Without LPS, pollen grains induced DC maturation and cytokine production without IL-12 secretion and the response, for which TLR 4 was dispensable, was greatest for the Cupressaceae family. Intranasal administration of Cupressaceae pollen in mice induced an elevation of serum IgE levels and airway eosinophil infiltration. Coadministration of ovalbumin with Cupressaceae pollen grains induced ovalbumin-specific IgE responses associated with eosinophil infiltration. The results suggest that modulation of DC responses by pollen differs among the plant families via (1) the promotion of DC maturation and cytokine production by direct contact and/or (2) the inhibition of IL-12 production by soluble factors. The strong DC stimulatory activity in vitro and IgE-inducing activity in mice support the clinical relevance of Cupressaceae pollen to allergies in humans.

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Immunobiology of grass pollen allergens

Cited by 39 publications

References 36 publications

IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis

IgE cross-reactivity between Lolium multiflorum and commercial grass pollen allergen extracts in Brazilian patients with pollinosis

Pollen Loads and Allergic Rhinitis in Darwin, Australia: A Potential Health Outcome of the Grass-Fire Cycle

Cupressaceae Pollen Grains Modulate Dendritic Cell Response and Exhibit IgE-Inducing Adjuvant Activity In Vivo

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