Immunobiotic Lactobacillus jensenii as immune-health promoting factor to improve growth performance and productivity in post-weaning pigs

Abstract

BackgroundImmunoregulatory probiotics (immunobiotics) have been proposed to improve piglets’ immune system to avoid intestinal infections and reduce unproductive inflammation after weaning. Previously, it was demonstrated that Lactobacillus jensenii TL2937 (LjTL2937) attenuated the inflammatory response triggered by activation of Toll-like receptor 4 (TLR-4) in porcine intestinal epithelial (PIE) cells and antigen presenting cells (APCs) from porcine Peyer’s patches (PP).ObjectiveIn view of the critical importance of PIE-APCs interactions in the regulation of intestinal immune responses, we aimed to examine the effect of LjTL2937 on activation patterns of APCs from swine PPs in co-cultures with PIE cells. In addition, we investigated whether LjTL2937 was able to beneficially modulate intestinal immunity of piglets after weaning to improve immune-health status.ResultsStimulation of PIE-APCs co-cultures with LjTL2937 increased the expression of MHC-II, CD80/86, IL-10, and Bcl-3 in CD172a+CD11R1- and CD172a+CD11R1high APCs. In addition, the TL2937 strain caused the upregulation of three negative regulators of TLR4 in PIE cells: MKP-1, Bcl-3 and A20. These changes significantly reduced the inflammatory response triggered by TLR4 activation in PIE-APCs co-cultures. The in vivo experiments using castrated male piglets (crossbreeding (LWD) with Landrace (L), Large Yorkshire (W) and Duroc (D))of 3 weeks of age demonstrated that feeding with LjTL2937 significantly reduced blood complement activity and C reactive protein concentrations while no changes were observed in blood leukocytes, ratio of granulocytes to lymphocyte numbers, macrophages’ activity and antibody levels. In addition, treatment with LjTL2937 significantly improved growth performance and productivity, and increased carcass quality.ConclusionsWe demonstrated that the use of immunobiotics strains like LjTL2937, as supplemental additives for piglets feedings, could be used as a strategy to maintain and improve intestinal homeostasis; that is important for the development of the pig and for health and performance throughout the productive life of the animal.

Probiotic roles ofLactobacillussp. in swine: insights from gut microbiota

“…, ) and regulation of the immune system (Suda et al . ). All of these traits impact the gut microbial populations such that opportunistic pathogens including Salmonella , Clostridia and Enterobacteria are kept in check, thus preventing infection and intestinal disorders (Nemcova et al .…”

“…An in‐depth analysis of the immune health promoting properties of L. jensenii TL2937 demonstrated that the use of immunobiotic strains as supplemental additives to piglets’ feed significantly reduced tenderness and improved juiciness and palatability of pork meat, along with reducing backfat thickness (Suda et al . ). In addition, Ross et al .…”

Lactobacillus acidophilus alleviates the inflammatory response to enterotoxigenic Escherichia coli K88 via inhibition of the NF-κB and p38 mitogen-activated protein kinase signaling pathways in piglets

“…Probiotics contribute to maintaining a healthy digestive system of the host via regulating both intestinal development and flora balance [1–3], some of which have been shown to enhance the host immune responses by regulating cytokine and chemokine production [2]. L. acidophilus strain is a well-characterized probiotic bacterium, which has been reported to improve the production performance of animals as well as enhance the immune responses [4–6].…”

La adición de Enterococcus faecium aumenta la respuesta inmune intestinal en cerdos en crecimiento

“…Es cada vez más reconocido que el epitelio intestinal está jugando un papel esencial en la homeostasis inmune, mediante la promoción de respuestas tolerogénicas y regulatorias. Estos resultados tienen implicaciones importantes sobre la regulación de la homeostasis de la mucosa por las bacterias probióticas (Suda et al, 2014), explicando la regulación de la respuesta inmune contra los probióticos. Como se observó en los resultados de esta investigación se presentó un proceso de tolerancia a favor de los probióti-cos y, por ende, una adaptación a dichos microorganismos.…”