Immunoblockage of 9-<i>O</i>-Acetyl GD3 Ganglioside Arrests the<i>In Vivo</i>Migration of Cerebellar Granule Neurons

Santiago, Marcelo F.; Costa, Marcos Romualdo; Mendez-Otero, Rosália

doi:10.1523/jneurosci.0116-03.2004

Cited by 30 publications

(33 citation statements)

References 32 publications

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“…GD3 and 9‐O‐acetyl GD3 are involved in migration of F‐11 neuroblastoma cells and rat cerebellar granule neurons, respectively (Zeng et al . 2000; Santiago et al . 2004), and HNK‐1 epitope is required for proper migration of neural crest cells (Bronner‐Fraser 1987; Nagase et al .…”

Section: Resultsmentioning

confidence: 99%

Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells

Yanagisawa

Taga

Nakamura

et al. 2005

Journal of Neurochemistry

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Neuronal and glial cells organizing the central nervous system (CNS) are generated from common neural precursor cells (NPCs) during neural development. However, the expression of cell-surface glycoconjugates that are crucial for determining the properties and biological function of these cells at different stages of development has not been clearly defined. In this study, we investigated the expression of several stagespecific glycoconjugate antigens, including several b-series gangliosides GD3, 9-O-acetyl GD3, GT1b and GQ1b, stagespecific embryonic antigen-1 (SSEA-1) and HNK-1, in mouse embryonic NPCs employing immunocytochemistry and flow cytometry. In addition, several of these antigens were positively identified by chemical means for the first time. We further showed that the SSEA-1 immunoreactivity was contributed by both glycoprotein and glycolipid antigens, and that of HNK-1 was contributed only by glycoproteins. Functionally, SSEA-1 may participate in migration of the cells from neurospheres in an NPC cell culture system, and the effect could be repressed by anti-SSEA-1 antibody. Based on this observation, we identified b1 integrin as one of the SSEA-1 carrier glycoproteins. Our data thus provide insights into the functional role of certain glycoconjugate antigens in NPCs during neural development. The mammalian central nervous system (CNS) is organized by a variety of cells such as neurons, glial cells (astrocytes and oligodendrocytes), microglias and ependymal cells. Developmentally, these cells are generated from a common progenitor cell type, the neural stem cell (NSC). NSCs are undifferentiated neural cells that are characterized by their high proliferative potential while retaining the capacity for self-renewal and multipotency, and they are capable of differentiating into neuronal and glial cells (Temple 1989;Weiss et al. 1996;McKay 1997;Temple and Alvarez-Buylla 1999;Gage 2000;Okano 2002). NSCs appear during neural plate formation and contribute to the major cell types of early neuroectoderms and the neural tube. As development proceeds, NSCs become progressively less abundant and more restricted neural precursor cells (NPCs) emerge. In adult brain, NSCs are located primarily in two regions: the subventricular zone of the lateral ventricle and the subgranular layer of the dentate gyrus in the hippocampus (Doetsch et al. 1999;Seri et al. 2001). NSCs/NPCs have been shown to provide a cellular reservoir for the formation of the CNS and replacement of cells lost during normal cell turnover. The fate of NSCs/NPCs, such as proliferation, differentiation, survival and death, is regulated by multiple intrinsic

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Section: Resultsmentioning

confidence: 99%

Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells

Yanagisawa

Taga

Nakamura

et al. 2005

Journal of Neurochemistry

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“…The function, behavior and even survival of cerebellar neurons are highly dependant on the cellular expression of the ganglioside species [26].…”

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confidence: 99%

Fully-automated chip-based nanoelectrospray tandem mass spectrometry of gangliosides from human cerebellum

Zamfir

Vukelić

Bîndilă

et al. 2004

J. Am. Soc. Mass Spectrom.

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The introduction of chip-based electrospray (ESI) ion sources into biological mass spectrometry (MS) addressed the fundamental issue of how to analyze minute amounts of complex biological systems. The automation of sample delivery into the MS combined with the chip-based ESI allows for high quality bioanalysis in a high-throughput fashion. These advantages have already been demonstrated in proteomics, direct screening of drugs and drug discovery. As part of our continuing effort to implement automated chip-based mass spectrometry into the field of complex carbohydrate analysis, we hereby report the development of a chipESI MS and MS/MS methodology for the screening of gangliosides. A strategy to characterize a complex ganglioside mixture from human cerebellar tissue, by automated ESIchip-quadrupole time-of-flight (QTOF) MS and MS/MS is presented here. The feasibility of this method, and the general experimental requirements for automated chipESI MS analysis of these carbohydrate species is

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“…Gangliosides have received attention because they can be abundantly found in the cell plasma membrane, where they are involved in signal transduction, cell adhesion, and migration (Santiago et al, , Yanagisawa, ; Miyakoshi et al, ). Since 9OacGD3 is high expressed in dissociated NS cells (Yanagisawa et al, ), we first confirmed the expression in mouse neurospheres, where 9OacGD3 was co‐expressed with both GFAP and nestin (Figure A and B, respectively), making these cells the gold standard.…”

Section: Resultsmentioning

confidence: 99%

Expression of ganglioside 9‐O acetyl GD3 in undifferentiated embryonic stem cells

Azevedo-Pereira

Morrot

Machado

et al. 2014

Cell Biology International

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Embryonic stem cells (ES cells) express a transient and heterogeneous pattern of molecules, which suggests a notable mechanism to control self-renewal avoid the differentiation into germ layers. We show that 9-O-acetyl GD3 (9OacGD3), a highly expressed b-series ganglioside in neural stem (NS) cells, is expressed in undifferentiated mouse ES cells in a heterogeneous fashion. After sorting, undifferentiated 9OacGD3(+) ES cell population had higher levels of nestin and Sox2 mRNA than the 9OacGD3(-) cells. Even with elevated expression of these neural transcription factors, 9OacGD3(+) cells did not give rise to more neural progenitors than 9OacGD3(-) cells. Expression of 9OacGD3 was recovered from 9OacGD3(-) cell population, demonstrating that expression of this ganglioside in mouse embryonic stem cells is transient, and does not reflect cell fate. Our findings show that the ganglioside 9OacGD3 is expressed heterogeneously and transiently in ES cells, and this expression corresponds to higher levels of Sox2 and Nestin transcripts.

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Immunoblockage of 9-O-Acetyl GD3 Ganglioside Arrests theIn VivoMigration of Cerebellar Granule Neurons

Cited by 30 publications

References 32 publications

Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells

Characterization of glycoconjugate antigens in mouse embryonic neural precursor cells

Fully-automated chip-based nanoelectrospray tandem mass spectrometry of gangliosides from human cerebellum

Expression of ganglioside 9‐O acetyl GD3 in undifferentiated embryonic stem cells

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