Immunochemical analysis of rabbit antibodies against vasopressin

The Specificity of Antisera for the Radioimmunoassay of Arginine-Vasopressin in Human Plasma and Urine during Water Loading and Dehydration

“…It has recently been shown (Czernichow et al, 1974) that it is possible to raise antisera to AVP with different immunological specificities. The present work supports this observation and shows the consequences for the measurement of AVP in plasma and urine.…”

“…Previous authors (Chard, 1973; Czernichow, Reinharz & Vallotton, 1974) have discussed the production of antisera to different antigenic sites in AVP, and the implication if these sites are different from those responsible for biological activity. However, the importance of the immunological identity of antisera to AVP in radioimmunoassay has never been demonstrated.…”

The vasotocin-like biological activity present in the bovine pineal is due to a compound different from vasotocin

“…The RIA for AVT/AVP developed in Geneva made use of an antibody against the tripeptidic carboxyterminal end of AVP or AVT, i.e., Pro-Arg-Gly(NH~). This antibody is probably also able to react with other compounds ending by Pro-Arg-Gly(NH-2) as suggested by the fact that it could react with the synthetic tripeptide Pro-Arg-Gly(NH2) (Czernichow et al, 1974). With this RIA we have found an immunoreactivity in the E5 fraction the dilution curve of which was exactly parallel to the standard (here AVP) (Fig.…”

Modified forms of vasopressin and oxytocin in a bovine pineal preparation

“…Purification of neurohypophyseal hormone-like structures and their dissociation from accompanying carrier proteins were performed by gel filtration in formic acid as in [12]. Details of the procedures and antigenic properties of the radioimmunoassays have been described [13- in the bovine pineal E5 preparation, using the AVP antiserum raised against the COOH-terminal tripeptide ending common to AVP-(l-9) and AVT-(I-9) [13]. Using radioimmunoassays for the 20-membered ring structure of AVP [ 141 and AVT [ 151, only amounts of immunoreactive AVP-like material could be measured in the extract ( fig.lB).…”

“…Final purification was achieved by subse- .0 mg bovine pineal ES dissolved in 2.0 ml of 0.1 M formic acid was applied on Sephadex G-100 (1.6 x 58 cm) run in 0.1 M formic acid containing 0.1% (w/v) BSA at a flow rate of 80 ml/h; fraction size, 2.0 ml. The presence of neurophysin and peptides with a COOH-terminal Pro-Arg-Gly(NHz) sequence was assayed by radioimmunoassay [13,16]. Arrows indicate elution positions of standard AVP-(l-9) and bovine pituitary neurophysin (NpII).…”