Immunochemical Analysis of Staphylococcal Antigenic Preparations

Further Studies of a Preparation of Protein A (Jensen’s Antigen A) from Staphylococcus aureus

“…In 5 of them the titer reached 32 or more, and in none was it less than 8.-In some of the gel precipitin assays with fraction A-l and the immune sera of this group two close and parallel bands were seen. This pattern has been noted previously in work with fraction A-l and sera from rabbits immunized with formalin-killed bacteria (18,19).…”

“…Fraction A-l consists almost entirely of protein (19) and con tains protein A. Various methods for purifying this antigen have been described (10,14,18,28) and one of the preparations ob tained (15) has been used in experiments to produce heterogenic antibody of the R am i z type, but it appears that no report has yet been published on the ability of the preparations to induce the pro duction of precipitins.…”

“…J ensen's antigen A was classified by us as a protein (17). After later elaboration of a technique for purifying the antigen (18), some chemical data about the purified product were given (19). The chemical composition of our preparation of J ensen's antigen A (called fraction A-1) closely resembled that of the product con currently prepared by Grov, Myklestad and Oeding (10).…”

Preparations of Purified Antigens from Staphylococcus Aureus in Biologic and Serologic Experiments

“…The results of chemical and serological analysis of the preparations obtained were published later (26). The fraction subjected to the most comprehensive analysis was the one containing J ensen's antigen A. G r o v , M yklestad and O eding (14) have also shown interest in this antigen.…”

Immunological Properties of Bacterial Cell Wall Mucopeptides.

“…Subsequently ( 2 ) we reported the extraction of a similar material from the urine of fasting rats (rat FMS). More recently, the separation of rat FMS into 2 chemically and biologically distinct fractions was accomplished in this laboratory and some of the characteristics of these fractions were determined (3,4). One fraction, FMS IA induced hypophagia but not fat mobilization when injected into rats; the second fraction, FMS IB, caused fat mobilization without an effect upon food intake.…”