Immunochemical Analysis of Streptococcus bovis, Strain s19, Cell Walls

Abstract

S U M M A R YTwo antigens, a glucose-rich capsular polymer and a cell wall carbohydrate consisting of N-acetylglucosamine, glucose and rhamnose, were isolated from Streptococcus bovis, strain s 19. N-acetylglucosamine and a haptenic fraction rich in glucosamine inhibited approximately 56 and 63 of the quantitative precipitin reaction between the s 19 cell wall carbohydrate and its homologous antiserum, respectively. A cross-reactivity between a preparation of azo-protein, which consisted of terminal P-N-acetylglucosaminide residues, and s 19 antiserum substantiated the fact that N-acetylglycosamine is one of the major antigenic determinants of the s 19 cell-wall carbohydrate.Haptenic inhibition studies have not revealed any structural feature of the antigenic determinant of the glucose-rich capsular polymer. INTRODUCTIONMedrek & Barnes (1962) have shown that Streptococcus bovis, a member of the group D streptococci, can be separated into at least 1 2 serological types on the basis of precipitin reactions. The type-specificity of 75 strains, in most instances, was attributed to a capsular-like material. However, in view of the fact that the immunochemical basis for the antigenic specificity of these type-specific substances has not been elucidated, it is possible that the observed type-specific reactions may also be dependent upon components of the cell wall other than the capsular material.Elliott (1960) reported the isolation of type-specific carbohydrates consisting of glucosamine, rhamnose and glucose from four serological types of group D streptococci. These type-specific carbohydrates were shown to be components of the cell wall and, therefore, were considered to be the structural counterpart of the group-specific carbohydrates of groups A, B, C, and G hemolytic streptococci.This report describes the isolation, purification and chemical properties af the type-

Immunological cross-reactivity between the vaccine and other isolates ofStreptococcus bovisandLactobacillus

“…A noticeable feature of the results was that all the three encapsulated strains of S. bovis (including Sb‐5) had similar CRIs (range: 7.3–12.4%), which were significantly lower than the CRIs of the non‐encapsulated strains (range: 28.9–56.1%). This is consistent with the earlier observations that both cell wall and capsule materials may contribute to the antigenicity of S. bovis [22–24]. The higher CRIs of the non‐capsulate strains may be due to the lack of capsular antigens of these strains since the antiserum for this study was prepared from cattle immunised with a vaccine containing encapsulated S. bovis strain Sb‐5 cells.…”

The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity of Streptococcus bovis/Streptococcus equinus Complex Members

“…In fact, colonization and antibody response of pigeons immunized with an SGP strain containing a thin irregular capsule (PDH 827) did not protect against a high-virulent strain SGG strain with a thick capsule (STR357). These findings indicate that supernatant proteins, glycans, fimbriae and the capsule may be involved in the induction of protective immunity against SGG infections but likely not across SBSEC species (Kane and Karakawa, 1969 ; Kane et al, 1972 ; Pazur and Forsberg, 1978 ; Pazur et al, 1978 ; Vanrobaeys et al, 1997 ; Kimpe et al, 2002 ).…”

Chemical Studies on the Structure of Mucopeptide Isolated from Streptococcus bovis

“…Schleifer and Kandler also have shown that S. cremoris 316a and 331a possess an L-alanyl-threonine dipeptide bridge (20). Recently, Kane et al reported that the mucopeptide of S. bovis, a member of the group D streptococci, contains a high concentration of threonine (7).…”