Immunochemical and biological characterization of outer membrane proteins of Porphyromonas endodontalis

Ogawa, Tomohiko; Kuribayashi, Sohei; Shimauchi, Hidetoshi; Toda, Tadao; Hamada, Shigeyuki

doi:10.1128/iai.60.11.4528-4533.1992

Cited by 8 publications

(5 citation statements)

References 44 publications

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“…IgG is more stable than IgA, thus making it a more appealing antibody for clarifying a history of infection. In patients with ELs, the major immunoglobulin against P. endodontalis antigens produced at the site is IgG (Ogawa et al 1992). Therefore, ELs might lack the nature of repeated or recent antigen exposure, which is required for elevated P. endodontalis IgA levels.…”

Section: Discussionmentioning

confidence: 99%

Association of Endodontic Lesions with Coronary Artery Disease

et al. 2016

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An endodontic lesion (EL) is a common manifestation of endodontic infection where Porphyromonas endodontalis is frequently encountered. EL may associate with increased risk for coronary artery disease (CAD) via similar pathways as marginal periodontitis. The aim of this cross-sectional study was to delineate the associations between EL and CAD. Subgingival P. endodontalis, its immune response, and serum lipopolysaccharide were examined as potential mediators between these 2 diseases. The Finnish Parogene study consists of 508 patients (mean age, 62 y) who underwent coronary angiography and extensive clinical and radiographic oral examination. The cardiovascular outcomes included no significant CAD ( n = 123), stable CAD ( n = 184), and acute coronary syndrome (ACS; n = 169). EL was determined from a panoramic tomography. We combined data of widened periapical spaces (WPSs) and apical rarefactions to a score of EL: 1, no EL ( n = 210); 2, ≥1 WPS per 1 apical rarefaction ( n = 222); 3, ≥2 apical rarefactions ( n = 76). Subgingival P. endodontalis was defined by checkerboard DNA-DNA hybridization analysis, and corresponding serum antibodies were determined by ELISA. In our population, 50.4% had WPSs, and 22.8% apical rarefactions. A total of 51.2% of all teeth with apical rarefactions had received endodontic procedures. Subgingival P. endodontalis levels and serum immunoglobulin G were associated with a higher EL score. In the multiadjusted model (age, sex, smoking, diabetes, body mass index, alveolar bone loss, and number of teeth), having WPSs associated with stable CAD (odds ratio [OR] = 1.94, 95% confidence interval [95% CI] = 1.13 to 3.32, P = 0.016) and highest EL score were associated with ACS (OR = 2.46, 95% CI = 1.09 to 5.54, P = 0.030). This association was especially notable in subjects with untreated teeth with apical rarefactions ( n = 59, OR = 2.72, 95% CI = 1.16 to 6.40, P = 0.022). Our findings support the hypothesis that ELs are independently associated with CAD and in particular with ACS. This is of high interest from a public health perspective, considering the high prevalence of ELs and CAD.

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Section: Discussionmentioning

confidence: 99%

Association of Endodontic Lesions with Coronary Artery Disease

et al. 2016

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“…A 41-kDa OmpA homolog has been identified in B. fragilis (30). P. endodontalis also has OmpA-like porins (31). These OmpA homologs were reported to have pore-forming activity.…”

Section: Discussionmentioning

confidence: 99%

Major outer membrane proteins from Porphyromonas gingivalis: strain variation, distribution, and clinical significance in periradicular lesions

Imai¹,

Murakami²,

Nagano³

et al. 2005

European J Oral Sciences

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Porphyromonas gingivalis has been implicated in both marginal periodontitis and periapical infection. This study examined the major outer membrane proteins, from P. gingivalis, which related to periradicular lesions. Outer membrane protein profiles of P. gingivalis ATCC 33277 and W83 were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and N-terminal amino acid analysis. Most outer membrane proteins, such as RagA, gingipains, and OmpA-like proteins, were found in both strains in a similar distribution pattern; however, the migration positions of Lys-gingipain and RagB were inverted in SDS-PAGE. Western blot analysis showed that RagA, RagB, and OmpA-like proteins were found in all of the P. gingivalis strains tested. The antiserum of W83 against RagB reacted poorly to some strains, such as ATCC 33277. When strains phylogenetically related to P. gingivalis were examined, RagA and OmpA homologs were immunologically detected in several strains. However, none of the RagB homologs were detected in any strain analyzed, suggesting that RagB is unique to P. gingivalis. To examine immunoreactive antigens in P. gingivalis, sera from patients with periradicular lesions were used. More than half of the sera showed strong reactions to P. gingivalis cell components, especially RagB. Our results indicate that a major outer membrane protein, RagB, is a possible virulence factor in periradicular lesions.

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“…Specific humoral immune response against several bacteria from the infected root canal has been investigated by the enzyme-linked immunosorbent assay (ELISA) method using crude bacterial components as antigens (Kettering et al 1991). Monoclonal and oligoclonal immunoglobulins were detected in periapical lesions (Matsumoto 1985) and periapical antibody-secreting cells against specific antigens of endodontopathic bacteria have been investigated by using enzyme-linked immunospot (ELISPOT) assay (Ogawa et al 1992). This evidence supports the possibility that`periapical lesion relevant' plasma cells may arise via selective homing and/or by the clonal proliferation and differentiation of specific B cells in this region.…”

Section: Host Defence Processesmentioning

confidence: 99%

Microbiological, pathological, inflammatory, immunological and molecular biological aspects of periradicular disease

Takahashi

1998

Int Endodontic J

104

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Multimicrobial infection of the dental pulp triggers inflammatory responses and ultimately causes bone destruction in the periradicular tissues. Besides bacteria, noxious substances such as degraded protein components and cholesterol could also act as antigens and elicit a host response, which can be harmful to periradicular tissues. Histologically, a dense infiltration of immunocompetent cells is seen in periradicular lesions and their host reactions may induce bone resorption. Polymorphonuclear leucocytes (PMN) and macrophages migrate to the periapical lesions and phagocytose pathogens as a first line of defence. Dead PMN are quickly phagocytosed by macrophages and this disposal system plays a role in maintaining chronicity of the lesions. The pathological roles of periradicular T cells have been assessed through analysing phenotypic markers of cell types, especially CD antigens, but the results are still controversial. Recently, technical developments in immunology and molecular biology have made it possible to investigate the pathogenesis of many diseases at molecular level. The investigation of functional analysis of the immune cells and their regulatory molecules such as apoptosis-associated molecules and adhesion molecules, will lead to a better understanding of the pathogenesis of periradicular lesions. The role of inflammatory mediators including antibodies, cytokines, matrix metalloproteinases, growth factors and arachidonic metabolism is becoming known for these lesions. Knowledge from these investigations improve the understanding of the pathological mechanisms of periradicular infections.

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Immunochemical and biological characterization of outer membrane proteins of Porphyromonas endodontalis

Cited by 8 publications

References 44 publications

Association of Endodontic Lesions with Coronary Artery Disease

Association of Endodontic Lesions with Coronary Artery Disease

Major outer membrane proteins from Porphyromonas gingivalis: strain variation, distribution, and clinical significance in periradicular lesions

Microbiological, pathological, inflammatory, immunological and molecular biological aspects of periradicular disease

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