Immunochemical characteristics of Streptococcus pneumoniae type 3 capsular polysaccharide glycoconjugate constructs correlate with its immunogenicity in mice model

Nuriev, R. I.; Galvidis, Inna A.; Burkin, Maksim A.

doi:10.1016/j.vaccine.2020.11.024

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Therefore, the conjugation of tetracyclines using Mannich condensation under neutral and alkaline pH conditions was preferable, in contrast to the recommendations in [ 20 ]. The inhibitory reactivity of conjugates in competitive ELISA may reflect the number of reactive epitopes on the tested conjugates [ 21 ] and hence their specific immunogenic activity ( Figure 1 B). Thus, BSA-TGC(f) conjugates prepared at different pH were compared for their inhibitory activity.…”

Section: Resultsmentioning

confidence: 99%

Tigecycline Immunodetection Using Developed Group-Specific and Selective Antibodies for Drug Monitoring Purposes

et al. 2023

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Tigecycline (TGC), a third-generation tetracycline, is characterized by a more potent and broad antibacterial activity, and the ability to overcome different mechanisms of tetracycline resistance. TGC has proven to be of value in treatment of multidrug-resistant infections, but therapy can be complicated by multiple dangerous side effects, including direct drug toxicity. Given that, a TGC immunodetection method has been developed for therapeutic drug monitoring to improve the safety and efficacy of therapy. The developed indirect competitive ELISA utilized TGC selective antibodies and group-specific antibodies interacting with selected coating TGC conjugates. Both assay systems showed high sensitivity (IC50) of 0.23 and 1.59 ng/mL, and LOD of 0.02 and 0.05 ng/mL, respectively. Satisfactory TGC recovery from the spiked blood serum of healthy volunteers was obtained in both assays and laid in the range of 81–102%. TGC concentrations measured in sera from COVID-19 patients with secondary bacterial infections were mutually confirmed by ELISA based on the other antibody–antigen interaction and showed good agreement (R2 = 0.966). A TGC pharmacokinetic (PK) study conducted in three critically ill patients proved the suitability of the test to analyze the therapeutic concentrations of TGC. Significant inter-individual PK variability revealed in this limited group supports therapeutic monitoring of TGC in individual patients and application of the test for population pharmacokinetic modelling.

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Section: Resultsmentioning

confidence: 99%

Tigecycline Immunodetection Using Developed Group-Specific and Selective Antibodies for Drug Monitoring Purposes

et al. 2023

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“…To obtain antibodies, a pair of NAT conjugates were prepared using TTd, a potent vaccine antigen and a carrier for various haptens [ 19 ]. The conjugation of TTd-adh-NAT and TTd-aca-NAT was directed to bond to different NAT sites, the carboxyl at position C25, and the mycosamine amine ( Figure 1 ).…”

Section: Resultsmentioning

confidence: 99%

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

2022

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The antifungal drug natamycin (NAT) is widely used in medicine and in the food industry as preservative E235 for a wide variety of foods. The risk of the development of resistance to NAT and its spread in relation to other polyene antibiotics is fraught with the emergence of incurable infections. This work is devoted to the development of an immunoassay to investigate the prevalence of NAT use for food preservation. Two immunogen designs based on tetanus toxoid, conjugated to NAT through different sites of hapten molecules, were compared in antibody generation. Assay formats using heterologous coating antigens were superior for both antibodies. The ELISA variant demonstrated the highest sensitivity (IC50 = 0.12 ng/mL), and a limit of detection of 0.02 ng/mL was selected for NAT determination. The optimized extraction procedure provided a recovery rate of 72–106% for various food matrixes with variations below 12%. Cyclodextrins, as well as NAT–cyclodextrin complex formulations, showed no interference with the quantification of NAT. One hundred and six food product brands, including baked goods, wines, beers, drinks, sauces, and yogurts, were tested to assess the prevalence of the undeclared use of NAT as a preservative. The screening examination revealed three positive yogurts with an undeclared NAT incorporation of 1.1–9.3 mg/kg.

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Tannic Acid, as a Structural Moiety Coupled to a Protein Antigen, Exhibiting a Molecular-structure Adjuvant Activity for Antibody Specificity Enhancement

Pedroza-Escobar

Molina-Ramírez

Cabral-Hipólito

et al. 2022

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Background: An antigen is a small foreign substance, such a microorganism structural-protein, that may trigger an immune response once inside the body. Antigens are preferentially used rather than a complete attenuated microorganism to develop safe vaccines. Unfortunately, not all antigens are able of inducing an immune response. Thus, new adjuvants seeking to enhance the antigen’s ability to stimulate immunity must be developed. Therefore,the objective of this work was to evaluate the molecular-structure adjuvant activity of tannic acid (TA) coupled to a protein antigen in Balb/c mice. Method: Bovine serum albumin (BSA) was used as an antigen. The coupling of BSA and TA was mediated by carbodiimide crosslinking, and verified by SDS-PAGE. Forty-two Balb/c mice were divided into seven groups including two controls without antigen, an antigen control, an adjuvant control, and two treatment groups. An additional group was used for macrophages isolation. A 30-day scheme was used to immunize the mice. The analysis of humoral immunity included immunoglobulin quantification, isotyping and antigen-antibody precipitation. The analysis of cell-mediated immunity included the quantification of nitric oxide from peritoneal macrophages and splenocytes’ proliferation assay, after treatment stimulation. Results: No differences were found in the antibodies’ concentration or isotypes induced with the conjugate or the pure BSA. However, an immunogenicity improvement (p<0.05) was observed through the specific anti-BSA antibody titles in mice immunized with the conjugate. Besides, macrophage activation (p<0.05) was detected when stimulated with the treatments containing TA. Conclusion: Tannic acid exhibited macrophages’ activation properties. Moreover, when TA is incorporated into the structure of a protein antigen such as BSA, an antibody specificity enhancement is observed. This as a consequence of antigen processing by activated antigen-presenting cells. These results showed the use of tannic acid as a novel candidate for vaccine molecular-structure adjuvant.

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Immunochemical characteristics of Streptococcus pneumoniae type 3 capsular polysaccharide glycoconjugate constructs correlate with its immunogenicity in mice model

Cited by 3 publications

References 27 publications

Tigecycline Immunodetection Using Developed Group-Specific and Selective Antibodies for Drug Monitoring Purposes

Tigecycline Immunodetection Using Developed Group-Specific and Selective Antibodies for Drug Monitoring Purposes

Immunoassay for Natamycin Trace Screening: Bread, Wine and Other Edibles Analysis

Tannic Acid, as a Structural Moiety Coupled to a Protein Antigen, Exhibiting a Molecular-structure Adjuvant Activity for Antibody Specificity Enhancement

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