Immunochemical comparison of cell-wall antigens of various viridans streptococci, including strain 2A2 + 3 hot from recurrent oral aphthous ulceration in man

Recurrent Aphthous Stomatitis

“…The initial L-form isolate from RAS patients was typed as S. sanguis (Barile et al, 1963), but later analysis disclosed that this organism was actually a strain of S. mitis (Hoover and Greenspan, 1983). While some studies have disclosed elevated serum antibody titers to viridans streptococci among RAS patients, other investigations have yielded contradictory results (Barile et al, 1968;Donatsky, 1976).…”

Humoral Responses and Cross-reactivity to Viridans Streptococci in Recurrent Aphthous Ulceration

“…The production, reactivity, and specificity of the rabbit anti-streptococcal sera are described in our previous study (Hoover and Greenspan, 1983 (Hoover and Greenspan, 1983 Indirect immunofluorescence. I.…”

“…End-point titers of sera from RAU and control subjects were determined by indirect immunofluorescence. Bacterial cells were grown and harvested as in our previous report (Hoover and Greenspan, 1983). Cells of each strain were treated with formalin and adjusted to an absorbance of 0.1 at 540 nm in distilled water.…”

“…Rabbit anti-streptococcal sera. Rabbit anti-streptococcal sera were titrated against the homologous bacterial strains as described in our previous report (Hoover and Greenspan, 1983 (Conover, 1980 Results.…”

“…S. sanguis and S. mitior are antigenically heterogeneous species (Rosan, 1973(Rosan, , 1976(Rosan, , 1978, and we have previously shown that Streptococcus strain 2A2+3HOT should be classified as a strain of S. mitior rather than S. sanguis (Hoover and Greenspan, 1983).…”