Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Ukena, Kazuyoshi; Oumi, T.; Morishita, Fumihiro; Furukawa, Yasuo; Matsushima, Osamu; Takahama, Hideki; Miyata, Seiji; Ikeda, Takako; Minakata, Hiroyuki; Nomoto, Kyosuke

doi:10.1007/s004410050842

Cited by 12 publications

(9 citation statements)

References 29 publications

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“…In a previous study, FMRFamide-IR sensory cells have also been shown to occur in Lumbricus body wall epithelium and have been clearly distinguished from pigment cells (Reglődi et al 1997a). In contrast, ETP-IR elements located in the mucosal epithelium of the gut have been described as endocrine cells (Ukena et al 1997). Few results are available concerning the occurrence of peptidergic sensory elements in invertebrates.…”

Section: Discussionmentioning

confidence: 93%

Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia): immunohistochemical and pharmacological studies

et al. 2005

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The distribution and chemical neuroanatomy of nervous elements and certain pharmacological-physiological characteristics of the innervation of the body wall in earthworms are described. Solitary sensory bipolar cells can be found among the epithelial cells. These bipolar cells contain serotonin, tyrosine hydroxylase, histamine, gamma-amino-butyric acid (GABA), Eisenia tetradecapeptide, proctolin or rhodopsin in various combinations. In the body wall, the plexus sub-muscularis is composed of nerve fibres only, whereas the plexus sub-epithelialis and muscularis also contain solitary nerve cells. These cells display histamine, GABA or neuropeptide Y immunoreactivity. The fibres of the three plexuses are reactive to serotonin, histamine, Eisenia tetradecapeptide, proctolin, GABA and neuropeptide Y antibodies. FMRFamide-immunoreactive fibres of the plexus muscularis originate from the central nervous system, whereas axons containing the other studied molecules are derived from both peripheral and central structures. High pressure liquid chromatography assays have revealed serotonin, dopamine and histamine in the body wall. Contractions of the body wall musculature can be elicited with serotonin and FMRFamide. Serotonin-evoked contractions are suppressed by the application of GABA. Serotonin acts both directly on the muscle cell receptors and indirectly through initiating transmitter release from the nervous elements, whereas the FMRFamide-induced contractions seem to be mediated through the muscle cell receptors only. The pharmacological profiles of the serotonin and GABA receptors resemble those of the vertebrate 5-HT(3) and GABA(B) receptor types. Our findings indicate that both the sensory and efferent system of the annelid body wall operate by means of a variety of neuroactive compounds, suggesting a complex role of signalling systems in the regulation of this organ.

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Section: Discussionmentioning

confidence: 93%

Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia): immunohistochemical and pharmacological studies

et al. 2005

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“…serotonin: Ehinger and Myhrberg 1971;Lengvári et al 1992;Rude 1969;Spörhase-Eichmann et al 1987a,b;octopamine: Csoknya et al 1996;Robertson 1975), amino acids and peptides (e.g. GABA: Telkes et al 1996; Eisenia-tetradecapeptide: Csoknya et al 2000;Ukena et al 1997;FMRFamide: Reglődi et al 1997) but some hormone-secreting cells are also known (corticotropin-releasing factor: Lubics et al 2003;gastrointestinal hormones: Reglődi et al 1999;Remy et al 1982;ACTH: Aros et al 1980;Kaloustin 1986). According to our double-staining experiments, all of the TSH-LI neurons in the CNS co-localize GABA.…”

Section: Comparison With the Distribution Of Neurotransmittersmentioning

confidence: 98%

Evidence for the presence of thyroid stimulating hormone, thyroglobulin and their receptors in Eisenia fetida: a multilevel hormonal interface between the nervous system and the peripheral tissues

et al. 2006

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The present study describes the localization and distribution of thyroid-stimulating hormone (TSH), thyroglobulin (TGB) and their receptors in Eisenia fetida (Annelida, Oligochaeta) as revealed by immunohistological methods. Immunopositive neuronal and non-neuronal cells are present in both the central nervous system and some peripheral organs (e.g. foregut and coelomocytes). TSH- and TGB-immunopositive neurons in the various ganglia of the central nervous system are differentially distributed. Most of the immunoreactive cells are found in the suboesophageal ganglion. The stained cells also differ in their shapes (round, oval, pear-shaped) and sizes (small, 12-25 microm; medium, 20-35 microm; large, 30-50 microm). In all ganglia of the central nervous system, TSH-positive neurons additionally show gamma aminobutyric acid (GABA) immunopositivity. Non-neuronal cells also take part in hormone secretion and transport. Elongated TSH-positive cells have been detected in the capsule of the central ganglia and bear granules or vacuoles in areas lacking neurons. Many of capillaries show immunoreactivity for all four tested antibodies in the entire central nervous system and foregut. Among the coelomocytes, granulocytes and eleocytes stain for TSH and its receptor and for TGB but not for thyroid hormone receptor. Most of the granulocytes are large (25-50 microm) but a population of small cells (10-25 microm) are also immunoreactive. None of the coelomocytes stain for GABA. We therefore suggest that the members of this hormone system can modify both metabolism and immune functions in Eisenia. Coelomocytes might be able to secrete, transport and eliminate hormones in this system.

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“…This concept of braingut peptides is well known from vertebrate studies. FMRFamide, proctolin, neuropeptide Y, Eisenia tetradecapeptide, urocortin, polypeptide PACAP, cholecystokinin, gastrin, and substance P have been detected in the enteric plexus of the earthworm gut (Barna et al 2001;Ukena et al 1997;Lubics et al 2003;Somogyvari-Vigh et al 2000;Reglodi et al 1999Reglodi et al , 2000Csoknya et al 1992). …”

Section: Introductionmentioning

confidence: 96%

“…Peptides, including numerous vertebrate-like peptides, have been identified in earthworms (Sundler et al 1977;Alumets et al 1979;Aros et al 1980a,b;Ukena et al 1997;Rzasa et al 1984;Kaloustian and Edmands 1986;Lkhider et al 1987;Al-Yousuf 1990;Hasegawa and Kobayashi 1991;Lengvari et al 1994;Oumi et al 1994;Reglodi et al 1997aReglodi et al ,b, 1999Reglodi et al , 2000Somogyvari-Vigh et al 2000;Lubics et al 2003). The separate structures of the CNS and stomatogastric nervous system of earthworms have been described in detail (Friedlander 1888;Retzius 1892;Ogawa 1939;Günther 1971a,b;Schürmann and Günther 1973;Barna et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Immunoreactivity to molluskan neuropeptides in the central and stomatogastric nervous systems of the earthworm, Lumbricus terrestris L.

Ierusalimsky

Balaban

2006

Cell Tissue Res

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Polyclonal antisera against two related command neuropeptides (CNP2 and CNP4) described in neurons of the terrestrial snail Helix were used in a study of the nervous system of the earthworm Lumbricus. The CNP-like peptides belong to the same neuropeptide subfamily and bear a C-terminal signature sequence Tyr-Pro-Arg-X. The distribution patterns of immunoreactive (IR) neurons were studied in the central nervous system (CNS), skin, and stomatogastric nervous system of the earthworm. IR neurons were found in all CNS ganglia, the patterns being similar for both antibodies used. Several clusters of IR cells were observed in the cerebral and subesophageal ganglia. In the ventral cord ganglia, the number of IR cells decreased in the rostro-caudal direction, and the IR cells sent their fibers mostly into the median fiber bundle. Segmental nerves contained no IR fibers. After injury of the worm body, the number of IR neurons in the CNS significantly increased. In the skin, IR sensory neurons were present in sensory buds. The stomatogastric ganglia only contained IR fibers. Numerous scattered IR neurons were found in the inner subepithelial layer of the esophagus and formed the enteric plexus in which the cell bodies displayed a segmentally repeated pattern. Possible involvement of CNP-like-IR neurons in central integratory processes, sensory processes, and the regulation of feeding is discussed.

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Immunochemical demonstration of Eisenia tetradecapeptide, a bioactive peptide isolated from the gut of the earthworm Eisenia foetida , in tissues of the earthworm

Cited by 12 publications

References 29 publications

Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia): immunohistochemical and pharmacological studies

Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia): immunohistochemical and pharmacological studies

Evidence for the presence of thyroid stimulating hormone, thyroglobulin and their receptors in Eisenia fetida: a multilevel hormonal interface between the nervous system and the peripheral tissues

Immunoreactivity to molluskan neuropeptides in the central and stomatogastric nervous systems of the earthworm, Lumbricus terrestris L.

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