Immunochromatographic Tests for Rapid Diagnosis of Noroviruses

Ushijima, Hiroshi; Thongprachum, Aksara; Okitsu, Shoko; Khamrin, Pattara

doi:10.1016/b978-0-12-804177-2.00011-7

Cited by 1 publication

(1 citation statement)

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“…Moreover, norovirus has shown negative effects and is considered an economic and health service burden . Although several immune-chromatography-based detection kits for norovirus have been introduced, concerns about their sensitivity remain. , In addition, these detection systems are time-consuming and have poor cost performance. , Because no robust replication system exists for norovirus cell culture, , norovirus-like particles (NoV-LPs) have been used as target biomaterials for norovirus-sensing studies. , …”

Section: Introductionmentioning

confidence: 99%

Binary Nanoparticle Graphene Hybrid Structure-Based Highly Sensitive Biosensing Platform for Norovirus-Like Particle Detection

Lee¹,

Takemura²,

Kato³

et al. 2017

ACS Appl. Mater. Interfaces

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Nanoparticle (NP)-decorated carbon nanotubes or graphenes (GRPs) have attracted attention because of their synergic properties such as enhanced electrical conductivity, magneto-optical effect, and plasmon resonance energy transfer. These hybrid carbon nanomaterials are widely used in sensing platforms to monitor target biomolecules, gases, and chemicals. In this study, binary nanoparticles, specifically gold (Au)/magnetic nanoparticle (MNP)-decorated graphenes (GRPs), were applied in a virus-sensing platform. This hybrid material exhibited multiple functionalities, including magnetic, plasmonic, and enhanced electrical properties. The Au/MNP-GRPs were synthesized in two steps at room temperature under mild conditions and magnetically deposited on a Pt-interdigitated electrode as electrical-sensing channels. After deposition onto the electrode, the surface of Au/MNP-GRPs was conjugated with norovirus antibody to produce a norovirus-like particle (NoV-LP)-sensing platform. NoV-LPs were successfully detected by the hybrid nanomaterial-sensing platform, exhibiting high sensitivity and specificity in a concentration range from 0.01 pg to 1 ng. In this case, the limit of detection was calculated as 1.16 pg/mL. Thus, the binary nanoparticle-decorated graphene shows excellent potential as an electrical-sensing platform for biomolecules.

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