Immunocompetent cells in patients with malignant disease.I. the lack of naturally occurring killer cell activity in the unfractionated circulating lymphocytes from patients with chronic lymphocytic leukemia (CLL)

Behelak, Youssef; Banerjee, Diponkar; Richter, M.

doi:10.1002/1097-0142(197612)38:6<2274::aid-cncr2820380614>3.0.co;2-i

Cited by 25 publications

(6 citation statements)

References 7 publications

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“…Natural killer activity provides a particularly interesting index of immunocompetence for several reasons. First, although its precise function is unknown, it is thought to provide a general host defense in combating host cells undergoing malignant transformation and also against viruses (58). Secondly, what is measured is a natural property of the cell rather than an artificially induced measure, such as response to mitogens.…”

Section: Cellular Immunitymentioning

confidence: 99%

The Influence of the Psyche and the Brain on Immunity and Disease Susceptibility: A Critical Review

Rogers

Dubey

Reich

1979

Psychosomatic Medicine

229

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In critically reviewing the sources of evidence connecting psyche and brain with the immune system, the authors include a brief review of current knowledge of the immune system, its interactions with the neuroendocrine system, and other factors influencing its regulation. These include developmental stages, aging, rhythmicity, and a variety of exogenous influences. The need for developing further information about normal base lines is emphasized. Against that background, many sources of data demonstrating connections between the central nervous system and the immune system are presented: indirect evidence from clinical and experimental illnesses involving the immune system, and direct changes in either humoral or cellular immunity after natural or experimental stress, conditioning, hypnosis, and direct brain stimulation. Possible mechanisms are discussed, as well as some important methodological issues for further research.

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Section: Cellular Immunitymentioning

confidence: 99%

The Influence of the Psyche and the Brain on Immunity and Disease Susceptibility: A Critical Review

Rogers

Dubey

Reich

1979

Psychosomatic Medicine

229

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“…Recently, abnormalities of antibody-dependent cell-mediated cytotoxicity (ADCC) and spontaneous lymphocyte-mediated cytotoxicity (SLMC) have been reported in patients with certain primary immunodeficiencies or with certain malignancies (19)(20)(21)(22)(23)(24)(25). The cell-mediated immune responses to tumors are quite complex because of the findings that T cells, non-T cells, and macrophages all may be involved (26).…”

mentioning

confidence: 99%

Spontaneous and antibody-dependent cell-mediated cytotoxicity by human T cell subpopulations.

Gupta¹,

Fernandes²,

Nair³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

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Human peripheral blood non-T cells, T cells, and their subpopulations (TM, T-y, To, Ty-depleted cells, and TM&-depleted cells) were assayed for their capacity to mediate spontaneous lymphocyte-mediated cytotoxicity (SLMC) Tar-rytown, NY) was added to the mononuclear cell suspension at a volume ratio of 1:2 and the mixture was incubated at 370 on a rotator for 30 min. Phagocytic cells were then depleted by subsequent centrifugation at 400 X g for 20 min. Lymphoid cells depleted of phagocytic cells were then collected from the interface and washed three times with balanced salt solution and resuspended in RPMI-1640 at 4 X 106 cells per ml.Purification of T Lymphocytes. Aliquots (1 ml) of phagocytic cell-depleted lymphoid cells were mixed with 0.25 ml of fetal calf serum (heat inactivated and absorbed with SRBC) and 1 ml of 1% neuraminidase-treated SRBC (25 units/ml of 5% SRBC). The mixture was incubated at 370 for 5 min, centrifuged at 200 X g for 5 min, and then incubated at 40 for 1 hr. The pellets were resuspended and left on ice for another 15 min. Rosetting T lymphocytes were separated from non-T lymphocytes on Ficoll/Hypaque density gradient by centrifugation at 480 X g for 20 min at 200. SRBC attached to T lymphocytes (pellet) were lysed with distilled water and double-strength minimal essential medium (Microbiological Associates, Bethesda, MD). T cells were washed three times in RPMI-1640 and were shown to contain 96-98% T cells as determined by rosette formation with SRBC and lack of surface immunoglobulin. Almost no peroxidase-positive cells were found in such preparations and viability was more than 98% as determined by trypan blue dye exclusion. Non-T cells contained 10% T cells, 60% B cells, and 30% third population or "K" cells.Ty cells were isolated either on the same day or after overnight incubation of T cells in medium containing 20% fetal calf serum; T/L cells were isolated after overnight incubation of purified T cells in medium containing fetal calf serum. These populations were separated by using ox erythrocytes (ORBC) coated with anti-ORBC IgM or IgG antibody.

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“…Much of this work has been done using established cell lines as the targets in SLMC assays, but reference is also made to results obtained using xenogeneic nontumor target cells, such as rabbit lymphoid cells (Behelak and Richter, 1975;Behelak et al, 1976) or chicken erythrocytes (Saksela et al, 1977), since these assay systems may well be measuring a similar functional population. Much of this work has been done using established cell lines as the targets in SLMC assays, but reference is also made to results obtained using xenogeneic nontumor target cells, such as rabbit lymphoid cells (Behelak and Richter, 1975;Behelak et al, 1976) or chicken erythrocytes (Saksela et al, 1977), since these assay systems may well be measuring a similar functional population.…”

mentioning

confidence: 99%

Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells

Pross

Baines

1977

Cancer Immunol Immunother

129

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Immunocompetent cells in patients with malignant disease.I. the lack of naturally occurring killer cell activity in the unfractionated circulating lymphocytes from patients with chronic lymphocytic leukemia (CLL)

Cited by 25 publications

References 7 publications

The Influence of the Psyche and the Brain on Immunity and Disease Susceptibility: A Critical Review

The Influence of the Psyche and the Brain on Immunity and Disease Susceptibility: A Critical Review

Spontaneous and antibody-dependent cell-mediated cytotoxicity by human T cell subpopulations.

Spontaneous human lymphocyte-mediated cytotoxicity against tumor target cells

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