Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish

Lazzari, Maurizio; Bettini, Simone

doi:10.1111/joa.12129

Cited by 19 publications

(22 citation statements)

References 99 publications

(139 reference statements)

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“…Our analysis, therefore, capitalizes on the deep evolutionary relationship between EmT, amygdala, and the sense of smell. Like rodents, zebrafish are macrosmatic animals whose reproductive behavior is hugely influenced by pheromones (Lazzari et al, 2014). Male zebrafish exposed to the female sex pheromone prostaglandin F2α (PGF2α) express stereotypic nudging behavior and conspecific male-male aggression (Sorensen et al, 1988;Yabuki et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

The Zebrafish Amygdaloid Complex – Functional Ground Plan, Molecular Delineation, and Everted Topology

2020

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In mammals and other tetrapods, a multinuclear forebrain structure, called the amygdala, forms the neuroregulatory core essential for emotion, cognition, and social behavior. Currently, higher circuits of affective behavior in anamniote nontetrapod vertebrates ("fishes") are poorly understood, preventing a comprehensive understanding of amygdala evolution. Through molecular characterization and evolutionary-developmental considerations, we delineated the complex amygdala ground plan of zebrafish, whose everted telencephalon has made comparisons to the evaginated forebrains of tetrapods challenging. In this radical paradigm, thirteen telencephalic territories constitute the zebrafish amygdaloid complex and each territory is distinguished by conserved molecular properties and structure-functional relationships with other amygdaloid structures. Central to our paradigm, the study identifies the teleostean amygdaloid nucleus of the lateral olfactory tract (nLOT), an olfactory integrative structure that links dopaminergic telencephalic groups to the amygdala alongside redefining the putative zebrafish olfactory pallium ("Dp"). Molecular characteristics such as the distribution of substance P and the calcium-binding proteins parvalbumin (PV) and calretinin (CR) indicate, that the zebrafish extended centromedial (autonomic and reproductive) amygdala is predominantly located in the GABAergic and isl1-negative territory. Like in tetrapods, medial amygdaloid (MeA) nuclei are defined by the presence of substance P immunoreactive fibers and calretininpositive neurons, whereas central amygdaloid (CeA) nuclei lack these characteristics. A detailed comparison of lhx5-driven and vGLut2a-driven GFP in transgenic reporter lines revealed ancestral topological relationships between the thalamic eminence (EmT), the medial amygdala (MeA), the nLOT, and the integrative olfactory pallium. Thus, the study explains how the zebrafish amygdala and the complexly everted telencephalon topologically relate to the corresponding structures in mammals indicating that an elaborate amygdala ground plan evolved early in vertebrates, in a common ancestor of teleosts and tetrapods.

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Section: Introductionmentioning

confidence: 99%

The Zebrafish Amygdaloid Complex – Functional Ground Plan, Molecular Delineation, and Everted Topology

2020

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“…To distinguish between Schwann cells and OEC, numerous molecular markers have been used to compare their immunochemical properties. Previous studies reported that both OEC and Schwann cells are immunopositive for S‐100 and GFAP and immunonegative for EMA . In contrast, OEC show negative staining for Leu7, which is positive in the peripheral nerve sheath and schwannomas .…”

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

“…Schwann cells and OECs have been described as two different types of cells that share microscopic aspects and many structural, behavioral, and immunohistochemical properties. 17,[32][33][34][35] Both OGS and OECT can present the same microscopic aspect of a typical schwannoma with elongated cells. To distinguish between Schwann cells and OEC, numerous molecular markers have been used to compare their immunochemical properties.…”

Section: Current Consensus Regarding Ogs and Oectmentioning

confidence: 98%

“…Previous studies reported that both OEC and Schwann cells are immunopositive for S-100 and GFAP and immunonegative for EMA. 33,34,36 In contrast, OEC show negative staining for Leu7, which is positive in the peripheral nerve sheath and schwannomas. 37 Based on these characteristics, the reactivity to Leu7 has been used as a diagnostic tool for OECT.…”

Section: Current Consensus Regarding Ogs and Oectmentioning

confidence: 99%

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Schwannoma‐like tumor in the anterior cranial fossa immunonegative for Leu7 but immunopositive for Schwann/2E

et al. 2016

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Schwannoma arising from the olfactory system, often called olfactory groove schwannoma (OGS), is rare, as the olfactory bulb and tract, belonging to the central nervous system, should lack Schwann cells. Another rare entity called olfactory ensheathing cell tumor (OECT) has been reported, which mimics clinical and radiological characteristics of OGS. Here, we report two rare cases of schwannoma-like tumor in the anterior cranial fossa that showed negative staining for Leu7, but positive staining for Schwann/2E, and discuss their origin. Two cases of mass lesions in the anterior cranial fossa in a 26-year-old man and a 24-year-old woman were successfully removed. Morphological examination of these tumors was compatible with a diagnosis of schwannoma. Immunohistochemically, both cases were negative for Leu7, yielding a diagnosis of OECT, but were positive for the schwannoma-specific marker, Schwann/2E. Immunohistochemical staining results in our two cases question the current assumption that OGS and OECT can be distinguished only by Leu7 staining pattern. In conclusion, the origins of OGS and OECT remain to be determined, and further studies in larger numbers of cases are needed to characterize these rare tumors in the anterior cranial fossa.

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The progress of olfactory ensheathing cells in the treatment of brain ischemia

Zhang

2018

Ibrain

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Brain ischemia is commonly encountered in clinical practice, and the treatment strategies for brain ischemia were limited. Olfactory ensheathing cells (OECs), as important glial cells, are available to the transplantation for the treatment of brain ischemia. A few studies confirmed that OEC administration could reduce the volume of infarct, promote neurological function recovery and nerve regeneration in rats with bran ischemia. OEC can express several trophic factors in vitro culture, and cell cultures can be regulated by using certain nutritional factors such as vascular endothelial growth factor (VEGF), insulin like growth factor‐1 (IGF‐1), and brain‐derived neurotrophic factor (BDNF). Additionally, other cultured nerve cells have a similar effect. These findings suggest neurotrophic factors (NTFs) may mainly contribute to the effect of OEC administration. However, there are still some problems on OEC transplantation for the treatment of brain ischemia. The objective of this review is to evaluate the current situation of OEC administration in brain ischemia, and discuss some problems on the clinic use of OEC for the treatment of cerebral ischemia.

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Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish

Cited by 19 publications

References 99 publications

The Zebrafish Amygdaloid Complex – Functional Ground Plan, Molecular Delineation, and Everted Topology

The Zebrafish Amygdaloid Complex – Functional Ground Plan, Molecular Delineation, and Everted Topology

Schwannoma‐like tumor in the anterior cranial fossa immunonegative for Leu7 but immunopositive for Schwann/2E

The progress of olfactory ensheathing cells in the treatment of brain ischemia

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